FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201988
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG20120.9960987781452364No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT18070.894607600451512No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT17760.8792601540685585No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC17430.8629225498544468No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT15420.763411687823039No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA14720.728756163732499No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA14160.7010317444600669No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC13550.6708319306097392No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT13550.6708319306097392No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC13420.6643959047072103No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC12790.6332059330257243No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC12640.6257797492920372No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC12360.6119175396558212No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT12290.6084519872467671No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG11680.5782521733964394No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG11390.5638948848446442No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA11280.5584490167732736No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT10670.5282492029229459No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT10460.517852545695784No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT10060.4980493890726182No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA9980.494088757747985No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC9530.4718102065469236No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC8930.442105471612175No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT8630.42725310414480067No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8540.42279739390458837No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT8510.421312157157851No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG8400.41586628908648043No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA8380.41487613125532213No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT8150.40348931619700174No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC7910.3916074222231024No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7800.3861615541517318No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG7750.38368615957383606No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7650.3787353704180446No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7610.37675505475572807No Hit
CAGGAAANAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC7540.3732895023466741No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7470.36982394993762No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7450.3688337921064618No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC7430.3678436342753035No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG7330.36289284511951203No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6970.3450700041586629No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6770.33516842584708No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6740.3336831891003426No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC6740.3336831891003426No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6710.33219795235360516No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6440.3188308216329683No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6430.3183357427173892No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6420.31784066380181003No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6370.3153652692239143No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6260.30991940115254374No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG6250.3094243222369646No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA6060.30001782284096085No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC5950.2945719547695903No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5910.2925916391072737No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC5890.2916014812761154No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG5880.29110640236053625No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5800.2871457710359031No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5720.28318513971126996No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT5630.27872942947105767No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5630.27872942947105767No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5400.26734261441273743No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5390.26684753549715823No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5360.26536229875042083No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5350.2648672198348417No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5340.26437214091926253No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA5280.2614016674257877No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5250.25991643067905024No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5240.2594213517634711No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5230.258926272847892No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5230.258926272847892No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC5230.258926272847892No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5180.25645087826999624No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC5110.25298532586094225No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT5090.25199516802978394No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5050.25001485236746734No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4880.24159851080262193No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4850.2401132740558845No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4810.23813295839356796No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC4780.2366477216468305No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4780.2366477216468305No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4770.23615264273125136No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG4760.2356575638156722No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC4690.23219201140661822No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4640.22971661682872252No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA4620.2287264589975642No Hit
CAGGAAANAGCTATGANCGGGGAGACTGGGCCATACGATAGGCTTAATCC4610.22823138008198507No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4580.22674614333524765No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG4560.22575598550408937No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4330.21436917044576906No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4270.21139869695229419No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4190.20743806562766107No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4170.2064479077965028No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4100.20298235538744874No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4040.2000118818939739No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4020.19902172406281562No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4010.19852664514723645No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4010.19852664514723645No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC4000.19803156623165735No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3950.1955561716537616No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3850.19060538249797018No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3790.1876349090044953No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3790.1876349090044953No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3740.18515951442659961No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3680.18218904093312474No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3680.18218904093312474No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT3660.18119888310196647No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3600.1782284096084916No Hit
CAGGAAANAGCTATGANCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT3550.17575301503059587No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3500.17327762045270015No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC3460.17129730479038358No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3440.1703071469592253No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3430.16981206804364615No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3400.16832683129690873No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT3350.165851436719013No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA3310.16387112105669643No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3250.1609006475632216No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3230.1599104897320633No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC3190.15793017406974671No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3180.15743509515416756No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3140.155454779491851No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3130.15495970057627187No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3130.15495970057627187No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3090.1529793849139553No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3070.151989227082797No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3060.15149414816721787No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2990.14802859575816385No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2920.14456304334910985No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2910.1440679644335307No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2900.14357288551795155No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2900.14357288551795155No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2890.14307780660237243No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2800.1386220963621601No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2780.13763193853100184No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2770.1371368596154227No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2760.13664178069984356No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2750.1361467017842644No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2750.1361467017842644No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2720.134661465037527No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2690.13317622829078954No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2650.13119591262847297No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2640.13070083371289384No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2600.12872051805057727No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2570.12723528130383985No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2570.12723528130383985No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2530.12525496564152327No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2520.12475988672594411No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2520.12475988672594411No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2500.12376972889478584No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCAGGAAACAGCT2460.12178941323246927No Hit
CAGGAAANAGCTATGANCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2360.11683862407667782No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2350.11634354516109868No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2340.11584846624551953No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2330.1153533873299404No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2260.11188783492088639No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2250.11139275600530725No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2240.11089767708972809No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2200.10891736142741153No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA2190.10842228251183239No Hit
CAGGAAANAGCTATGANNCATGTGCCCCACTGATGCTCTCAATGTGTACT2180.10792720359625324No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2140.10594688793393668No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2130.10545180901835752No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2130.10545180901835752No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2120.10495673010277838No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2100.10396657227162011No Hit
CAGGAAANAGCTATGANCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2090.10347149335604094No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT2080.10297641444046181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)