FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177968
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA33481.8812370763283286No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA29001.6295064281219094No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT27541.547469207947496No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG26961.514879079385058No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT25671.442394138272049No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG25241.4182324912343791No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG24241.3620426144025892No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG23291.3086622314123888No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA22931.2884338757529443No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC20881.1732446282477749No Hit
TGTAAAANGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA20531.1535781713566484No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA20141.1316641193922503No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA19991.1232356378674817No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC19481.0945788006832688No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC19101.0732266474871888No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC19071.0715409511822351No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT18311.0288366447900745No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC18281.027150948485121No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC17991.010855884203902No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC17290.9715229704216488No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT16690.9378090443225748No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC16210.9108379034433156No Hit
TGTAAAANGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT15210.8546480266115257No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA14390.8085723276094579No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA13620.7653061224489796No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT13490.7580014384608469No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13420.7540681470826216No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12780.718106625910276No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC12700.7136114357637329No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA12430.6984401690191495No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC12170.6838308010428842No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC12150.6827070035062484No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12100.6798975096646588No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG12060.6776499145913872No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA11900.6686595342983008No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG11880.667535736761665No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGATGGCACTGA11350.6377551020408163No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG11270.6332599118942731No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC10990.617526746381372No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT10860.6102220623932392No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT10850.6096601636249213No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGATGGCACTGA10560.5933650993437022No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG10280.577631933830801No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC10220.5742605412208936No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT10010.5624606670862178No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT9900.5562797806347208No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC9780.5495369954149061No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT9630.5411085138901375No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC9570.5377371212802301No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC9530.5354895262069586No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC9360.5259372471455542No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG8940.5023374988762024No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC8840.49671851119302346No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC8830.4961566124247056No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT8770.49278521981479817No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT8720.48997572597320865No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8720.48997572597320865No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC8700.4888519284365729No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8500.47761395307021487No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC8190.46019509125235997No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC7870.4422143306661872No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7800.43828103928796186No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT7710.4332239503731008No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT7630.4287287602265576No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7540.4236716713116965No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT7380.41468129101861007No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7250.40737660703047734No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC7240.4068147082621595No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC7230.40625280949384157No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA7190.40400521442057No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7180.4034433156522521No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC7090.398386226737391No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7000.3933291378225299No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC7000.3933291378225299No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6970.3916434415175762No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA6950.3905196439809404No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA6820.3832149599928077No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA6700.37647217477299294No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA6470.3635485031016812No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG6420.3607390092600917No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG6170.3466915400521442No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG6010.33770115975905785No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA5820.3270250831610177No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5750.3230917917827924No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5410.3039872336599838No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5340.3000539422817585No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5310.29836824597680484No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC4890.27476849770745304No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA4610.2590353321945518No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC4530.2545401420480086No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC4380.24611166052324013No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG4380.24611166052324013No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT4220.23712128023015375No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT4060.22813089993706737No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG4010.22532140609547785No Hit
TGTAAAANGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG3930.22082621594893465No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3860.21689292457070936No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT3830.21520722826575564No Hit
TGTAAAANGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC3570.20059786028949023No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3490.19610267014294702No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT3480.19554077137462914No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG3360.18879798615481436No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT3260.18317899847163535No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC3230.18149330216668164No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT3020.16969342803200574No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG2920.16407444034882676No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG2840.15957925020228356No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2810.15789355389732987No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC2810.15789355389732987No Hit
TGTAAAANGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC2800.15733165512901195No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG2790.15676975636069404No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG2760.15508406005574038No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2630.14777937606760766No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2620.14721747729928977No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2570.14440798345770026No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2530.14216038838442865No Hit
TGTAAAANGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT2520.14159848961611077No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2450.13766519823788548No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2430.13654140070124965No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG2410.13541760316461388No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2370.13317000809134225No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2360.13260810932302436No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG2340.13148431178638856No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT2210.12417962779825586No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT2210.12417962779825586No Hit
TGTAAAANGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT2130.11968443765171266No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG2070.11631304504180527No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2030.11406544996853368No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2010.11294165243189787No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1950.10957025982199046No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1930.10844646228535468No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1870.10507506967544727No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT1810.10170367706553987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)