FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136911
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT30762.2467150192460794No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA27502.0086041296900907No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT26581.9414071915331856No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG26031.901235108939384No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT24071.7580764146051082No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA23821.739816377062471No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC20841.522156729554236No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT20681.5104703055269482No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA18351.3402867556295697No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA18121.3234875210903434No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC17571.2833154384965415No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC17351.2672466054590208No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT16171.1810592282577734No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT15231.1124014870974575No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC14971.0934110480531147No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG14711.0744206090087722No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT13610.9940764438211686No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA13450.9823900197938807No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT13240.9670515882580656No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA13110.9575563687358941No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA13080.9553651642307777No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG12590.9195754906472087No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG11410.8333881134459613No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG11250.8217016894186734No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA10610.7749559933095223No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT10490.7661911752890563No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT10480.7654607737873509No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT10450.7632695692822344No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT9440.6894990176099802No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC8960.6544397455281168No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG8680.6339885034803632No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA8280.6047724434121436No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT7930.5792083908524516No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA7880.5755563833439241No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG7830.5719043758353968No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT7320.5346538992484168No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC7250.5295410887364784No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT7220.527349884231362No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT7160.5229674752211291No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC7100.5185850662108961No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT7090.5178546647091906No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCACCTTGCTTC6900.5039770361767864No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG6840.4995946271665535No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA6690.48863860464097114No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG6620.4835257941290328No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA6570.4798737866205053No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT6470.47256977160345043No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT6400.46745696109151197No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC6280.4586921430710462No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA6190.45211852955569676No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT6120.44700571904375835No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA6080.4440841130369364No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT6080.4440841130369364No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA5950.43458889351476504No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA5780.4221720679857718No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG5740.41925046197894983No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5570.40683363644995657No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC5550.40537283344654557No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCGTGTGCGCT5480.40026002293460716No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5360.39149520491414136No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA5350.39076480341243586No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAGCTGCCTTG5350.39076480341243586No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG5340.39003440191073036No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT5340.39003440191073036No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC5280.3856519929004974No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT5080.37104396286638763No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC5040.3681223568595657No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC5010.36593115235444923No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG4870.3557055313305724No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC4830.35278392532375047No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4600.33598469078452425No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT4580.33452388778111325No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT4520.33014147877088035No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC4400.3213766607504145No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4340.3169942517401816No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC4330.3162638502384761No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG4230.30895983522142123No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG4090.2987342141975444No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTCCCCACCTCCT4040.29508220668901697No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC4000.292160600682195No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT3980.29069979767878407No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3960.28923899467537306No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC3930.2870477901702566No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3840.2804741766549072No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC3840.2804741766549072No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC3840.2804741766549072No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC3750.27390056313955785No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA3600.2629445406139755No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT3590.26221413911227004No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGT3520.25710132860033164No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGATGCAGTCAAGAG3470.2534493210918042No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT3460.2527189195900987No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC3220.23518928354916696No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3210.2344588820474615No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT3180.23226767754234504No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCAGTGGAAGG3060.22350285952187918No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2940.21473804150141332No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA2910.2125468369962969No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT2870.20962523098947491No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC2870.20962523098947491No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2780.20305161747412553No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT2650.19355639795195417No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC2620.19136519344683772No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2590.18917398894172124No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC2580.18844358744001577No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG2530.18479157993148834No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA2520.18406117842978284No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAGCCAA2490.1818699739246664No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTGCCTATAAT2470.1804091709212554No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2450.17894836791784444No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA2420.17675716341272796No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTGCCTCCCGAC2380.17383555740590603No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG2340.17091395139908408No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2320.1694531483956731No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAGAATCTGAGA2310.1687227468939676No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC2270.16580114088714568No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC2260.16507073938544017No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2210.16141873187691275No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2210.16141873187691275No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCCAGGCTCA2190.15995792887350177No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCGGGTTCCCA2180.15922752737179627No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA2120.15484511836156334No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2120.15484511836156334No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCACTGGTCCGT2090.1526539138564469No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGAGCCGGCACAAC2090.1526539138564469No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGCTGCCCTGC2040.14900190634791946No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGTCTCCCCAGG2040.14900190634791946No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG2020.14754110334450848No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGTGAAGCAC2020.14754110334450848No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGAAGAACAGCGAG1980.14461949733768653No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGCCTCATGTCC1940.14169789133086458No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1930.1409674898291591No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT1930.1409674898291591No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC1890.13804588382233712No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1890.13804588382233712No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGCCCGCACCAG1880.13731548232063165No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA1870.13658508081892617No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC1830.1336634748121042No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1760.12855066430016582No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCACCGCCAAGA1760.12855066430016582No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1740.1270898612967548No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1720.12562905829334384No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1720.12562905829334384No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCCCAC1700.12416825528993287No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG1700.12416825528993287No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCGCCTCCCC1690.12343785378822739No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTAGGCTAGCA1650.12051624778140545No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA1620.11832504327628898No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGA1590.11613383877117252No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG1570.11467303576776156No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCAAAGGAGAT1560.11394263426605605No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTTCCTGCGGAGAG1550.11321223276435056No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT1510.11029062675752863No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCAGCATTGTGCCT1500.10956022525582312No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCATATTCCTCTA1480.10809942225241216No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1470.10736902075070666No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1460.10663861924900117No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1450.1059082177472957No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT1440.1051778162455902No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG1430.1044474147438847No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1430.1044474147438847No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT1410.10298661174047373No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA1390.10152580873706277No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTAGTGGTACAGTC1390.10152580873706277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)