FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162845
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG42492.609229635543001No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC32381.988393871472873No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC28041.7218827719610672No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT27881.7120574779698485No Hit
CAGGAAACAGCTATGACCAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG27501.6887224047407043No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA25571.5702047959716297No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA23531.4449322975835919No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA22211.363873622156038No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA20551.2619361969971445No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA20031.230003991525684No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT19871.2201786975344653No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG19731.2115815652921489No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC18111.11210046363106No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG17961.1028892505142929No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG17771.0912217138997207No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG17771.0912217138997207No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA17741.0893794712763671No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG17611.081396419908502No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA17551.077711934661795No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT16020.9837575608707667No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT15520.9530535171482083No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT15430.9475267892781479No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC14510.8910313488286408No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA14490.8898031870797385No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA14140.8683103564739477No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA13680.8400626362491941No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC13550.8320795848813289No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG13460.8265528570112683No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA13210.8112008351499892No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC13140.8069022690288311No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG12740.7823390340507845No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACACCTGGCCTTCAA12400.7614602843194449No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT12040.7393533728392029No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG12010.7375111302158495No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG11530.7080352482421934No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG10870.6675059105284166No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA10720.6582946974116491No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG10690.6564524547882956No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA10470.64294267555037No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC10080.6189935214467746No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG9900.6079400657066535No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC9600.5895176394731186No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC9450.5803064263563511No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG9320.5723233749884861No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG9240.5674107279928766No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC8870.5446897356381836No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA8850.5434615738892812No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGTGATTCAGTAG8840.54284749301483No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC8780.5391630077681231No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG8680.5330221990236114No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGGCGCAGCCACC8590.5274954711535509No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGCCGGGGTATGG8570.5262673094046486No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA8460.5195124197856857No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG8160.5010899935521509No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT8050.494335103933188No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT8000.4912646995609322No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG7740.47529859682520187No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC7640.4691577880806902No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC7610.4673155454573368No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT7380.45319168534495996No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT7330.4501212809727041No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7290.4476649574748994No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCGTGTGCGCTGGA7200.44213822960483895No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT6860.4212594798734993No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGACATGGCCA6660.40897786238447603No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGCTCCCCAGG6640.40774970063557375No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA6600.4052933771377691No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGTGGCTGAGGTG6510.39976664926770855No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT6400.3930117596487457No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT6360.39055543615094107No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG6330.38871319352758754No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6240.3831864656575271No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG5970.36660628204734563No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT5590.34327120881820133No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC5520.3389726426970432No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAGCCAT5510.33835856182259205No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCACAGCTGCTGC5300.32546286345911757No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGTGATGG5130.31502348859344775No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG5110.3137953268445454No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTGTACTCAGAAG4970.3051981946022291No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCACA4950.30397003285332674No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT4770.2929165771132058No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC4770.2929165771132058No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC4670.2867757683686942No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCATGAGCCCAG4660.28616168749424303No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG4650.28554760661979184No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG4640.28493352574534064No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACACCTCCTCTGG4530.27817863612637783No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT4440.27265190825631735No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC4340.2665110995118057No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCGTGTGCGCTGG4320.26528293776290335No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGTCCAGAGGCT4280.26282661426509873No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCACCCAGCACAGG4260.2615984525161964No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGACGTGTCCGCT4250.2609843716417452No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTGGGGACTGGA4220.25914212901839173No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC4140.2542294820227824No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCCCGTGTGCGCTG4120.25300132027388006No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC3970.24379010715711258No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTACTTGAGCTCAG3930.24133378365930794No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG3910.2401056219104056No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA3870.23764929841260093No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC3860.23703521753814977No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC3820.2345788940403451No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3800.23335073229144276No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC3750.23028032791918696No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCGCGGGAAAG3580.21984095305351714No Hit
CAGGAAACAGCTATGACCCCCCTCCGCTGGTTGAATGGGCTGTCTTGGCT3510.21554238693235897No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT3460.21247198256010316No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC3440.21124382081120086No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC3370.20694525469004266No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCTCGCCAAGA3360.20633117381559152No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA3270.200804445945531No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG3250.1995762841966287No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT3230.19834812244772637No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC3140.1928213945776659No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAAGAGGGGGCAG3130.19220731370321473No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACATCAAATGC3000.18422426233534955No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCTGGGTATGTGG2990.1836101814608984No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGCCGAGACCCTGA2970.18238201971199605No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2950.18115385796309375No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG2880.17685529184193557No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC2850.1750130492185821No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTAACAAACTCCC2840.17439896834413093No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAACCAGTGAC2820.1731708065952286No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2690.16518775522736345No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACAGTAACT2600.15966102735730295No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGACCAATGCC2600.15966102735730295No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA2590.15904694648285178No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT2580.1584328656084006No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2340.14369492462157268No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTAGGAGGCTGTC2330.14308084374712152No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC2330.14308084374712152No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG2050.12588657926248886No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC2050.12588657926248886No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG2050.12588657926248886No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1950.1197457705179772No Hit
CAGGAAACAGCTATGACCAACTTTTAGGTAGAGCCAAAGAACTGGCCGTC1930.11851760876907488No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC1810.1111486382756609No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTTTTACACCA1750.10746415302895392No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA1710.10500782953114926No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC1650.10132334428444227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)