FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125041
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA39423.152565958365656No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT25052.003342903527643No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG21151.691445205972441No Hit
TGTAAAACGACGGCCAGTCCTCTGGCATGCTGGTAGACTTGTGCCTGTTA20141.6106716996825043No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC19541.5626874385201655No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT18981.5179021281019827No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT17041.3627530170104205No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT16081.2859781991506785No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG16041.2827792484065226No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG15161.2124023320350925No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA14931.194008365256196No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT14021.1212322358266489No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG13961.116433809710415No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC13771.1012387936756745No Hit
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA13071.045257155652946No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC12170.9732807639094377No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT12060.9644836493630089No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT12040.962884173990931No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC10890.8709143400964483No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC10620.849321422573396No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC10370.8293279804224214No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG9260.7405570972720947No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC8460.6765780823889764No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA8400.6717796562727425No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT8040.6429890995753392No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7840.6269943458545597No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGTACCCCTGTGC7690.614998280563975No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA7660.612599067505858No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA7450.5958045760990395No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC7200.575811133948065No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG7160.572612183203909No Hit
TGTAAAANGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG6990.5590166425412465No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATAGCT6950.5558176917970905No Hit
TGTAAAANGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT6610.5286266104717653No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT6500.5198294959253366No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC6210.4966371030302061No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT6180.4942378899720891No Hit
TGTAAAANGACGGCCAGTCCTCTGGCATGCTGGTAGACTTGTGCCTGTTA6130.49023920154189427No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT6060.4846410377396214No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA5970.47744339856527057No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT5910.4726449724490367No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC5870.4694460217048808No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT5470.43745651426332166No Hit
TGTAAAANGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG5200.4158635967402692No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT5080.4062667445078014No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT4940.39507041690325573No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA4920.3934709415311778No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA4850.38787277772890494No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG4830.38627330235682694No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC4800.38387408929871003No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT4740.37907566318247615No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC4580.36627986020585246No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC4540.3630809094616966No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT4400.3518845818571508No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTAGCTAG4390.35108484417111185No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA4380.3502851064850729No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC4360.34868563111299494No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT4350.34788589342695597No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC4340.34708615574091695No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG4340.34708615574091695No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT4320.345486680368839No Hit
TGTAAAANGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT4260.3406882542526051No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG4200.33588982813637125No Hit
TGTAAAANGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT4160.33269087739221537No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4070.3254932382178645No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT4060.32469350053182555No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA4030.3222942874737086No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT4010.32069481210163064No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGACACCGTAACTGT3980.31829559904351373No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC3860.308698746811046No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT3860.308698746811046No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG3840.307099271438968No Hit
TGTAAAANGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT3820.30549979606689004No Hit
TGTAAAANGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC3820.30549979606689004No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC3780.3023008453227341No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT3750.2999016322646172No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC3690.2951032061483833No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA3650.2919042554042274No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG3600.2879055669740325No Hit
TGTAAAANGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG3480.27830871474156477No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC3470.2775089770555258No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG3150.25191737110227846No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA3110.24871842035812253No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA3060.24471973192792765No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA3050.24391999424188868No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGGCCTGG3010.24072104349773274No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT3000.23992130581169377No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG2970.23752209275357683No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC2930.23432314200942092No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGACACTGTGAGGCCC2910.23272366663734295No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2830.22632576514903113No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC2760.22072760134675828No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG2590.20713206068409562No Hit
TGTAAAANGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG2530.20233363456786174No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2520.20153389688182277No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGCATTGGTCTGG2440.19513599539351092No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCCATCTCCATTT2410.19273678233539399No Hit
TGTAAAANGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATAGCT2400.19193704464935502No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA2390.19113730696331604No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT2380.19033756927727705No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC2370.18953783159123808No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT2330.18633888084708217No Hit
TGTAAAACGACGGCCAGTAGTACGAAAAGTAGGTGGCCTTACTGGTAGCA2320.18553914316104317No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG2300.18393966778896523No Hit
TGTAAAANGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT2270.1815404547308483No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGCCAGCCCCCCAC2250.17994097935877032No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGCTTGACACGG2230.17834150398669235No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCAAATGCGTGCA2220.17754176630065338No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA2210.1767420286146144No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG2160.1727433401844195No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG2150.17194360249838053No Hit
TGTAAAANGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA2120.1695443894402636No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCATGGCCTGGTGGT2080.16634543869610768No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGATGAAACCTCA2060.16474596332402971No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT2030.16234675026591278No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA2030.16234675026591278No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2030.16234675026591278No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT1900.15195016034740605No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA1890.15115042266136705No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGGGCTGCTCTGTC1890.15115042266136705No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGACACCGTAACTAT1850.14795147191721114No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT1830.1463519965451332No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGGGTACCCCTGTGC1800.14395278348701626No Hit
TGTAAAANGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA1730.1383546196847434No Hit
TGTAAAANGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG1680.1343559312545485No Hit
TGTAAAANGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC1670.13355619356850953No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGACCTTCCAC1670.13355619356850953No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC1660.13275645588247056No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGCCCCAAGGAT1640.1311569805103926No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGAGAGCGGTTCC1630.13035724282435363No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT1630.13035724282435363No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT1630.13035724282435363No Hit
TGTAAAANGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT1610.12875776745227566No Hit
TGTAAAANGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT1610.12875776745227566No Hit
TGTAAAANGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT1590.12715829208019772No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1570.12555881670811975No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTGGGCTCCAGTA1510.12076039059188586No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT1450.11596196447565199No Hit
TGTAAAANGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA1450.11596196447565199No Hit
TGTAAAANGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT1440.115162226789613No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1440.115162226789613No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA1430.11436248910357402No Hit
TGTAAAANGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT1420.11356275141753505No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC1390.11116353835941811No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT1390.11116353835941811No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT1390.11116353835941811No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1380.11036380067337914No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG1360.10876432530130117No Hit
TGTAAAANGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC1350.1079645876152622No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA1330.10636511224318423No Hit
TGTAAAANGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTAGCTAG1320.10556537455714526No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC1290.10316616149902832No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGGGGCCATCATCC1260.10076694844091139No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA1260.10076694844091139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)