FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125041
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT7910.6325925096568326No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT6540.5230284466694924No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT6530.5222287089834534No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT6390.5110323813789077No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT6350.5078334306347518No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT6050.48384130005358245No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5890.47104549707695875No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5840.46704680864676384No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5530.4422549403795555No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT5300.423860973600659No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT5190.41506385905423027No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5060.4046672691357235No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4710.3766764501243592No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4680.3742772370662423No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT4550.36388064714773555No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT4410.35268431954318985No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4270.3414879919386441No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT4170.33349061507825434No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC4040.3230940251597476No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3840.307099271438968No Hit
NNGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG3790.30310058300877313No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT3770.30150110763669513No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3750.2999016322646172No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT3560.2847066162298766No Hit
NNGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAAGTTG3510.2807079277996817No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC3450.27590950168344786No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3360.268711862509097No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3320.26551291176494113No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3180.25431658416039543No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3160.25271710878831743No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3130.2503178957302005No Hit
NNGGANNNNGNTATGANNGATGGCAGAATGGAAACATGGCAGCTGCCTTG3100.24791868267208356No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3010.24072104349773274No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT2990.2391215681256548No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2940.2351228796954599No Hit
NAGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT2910.23272366663734295No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2890.231124191265265No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT2870.22952471589318704No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2790.2231268144048752No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2720.21752865060260235No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2720.21752865060260235No Hit
NAGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2600.2079317983701346No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2510.2007341591957838No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2490.1991346838237058No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT2490.1991346838237058No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2490.1991346838237058No Hit
NNGGANNNNGNTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG2470.19753520845162786No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2430.19433625770747195No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2410.19273678233539399No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2390.19113730696331604No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA2350.1879383562191601No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC2300.18393966778896523No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2220.17754176630065338No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2190.17514255324253644No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGGTACCCGTGTC2170.17354307787045847No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT2160.1727433401844195No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2130.17034412712630256No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT2110.1687446517542246No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGACAGGAAACA2070.16554570101006869No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2070.16554570101006869No Hit
NAGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2060.16474596332402971No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT2040.16314648795195175No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2040.16314648795195175No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2040.16314648795195175No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2020.1615470125798738No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2000.15994753720779584No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1970.1575483241496789No Hit
NAGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1950.15594884877760093No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1940.15514911109156196No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1900.15195016034740605No Hit
NNGGANNNNGNTATGNNNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG1870.1495509472892891No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1860.1487512096032501No Hit
NAGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG1850.14795147191721114No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1850.14795147191721114No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1830.1463519965451332No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1820.1455522588590942No Hit
NNGGANNNNGNTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1810.14475252117305523No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAAGGGGACATGGCC1810.14475252117305523No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1810.14475252117305523No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1780.1423533081149383No Hit
NNGGANNNNGNTATGNNNAACCAACACTGCTGCTAATTGTGTTTGTGTCC1780.1423533081149383No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1750.13995409505682135No Hit
NAGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1690.1351556689405875No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1680.1343559312545485No Hit
NNGGANNNNGNTATGNNNACAAACGCAAATGGCTTTTAGCATTTCACAGG1640.1311569805103926No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1600.12795802976623669No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1590.12715829208019772No Hit
NAGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1590.12715829208019772No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGGTACCCGTGTC1580.12635855439415872No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1560.12475907902208076No Hit
NNGGANNNNGNTATGNNNTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1550.12395934133604178No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1550.12395934133604178No Hit
NNGGANNNNGNTATGNNNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1530.12235986596396382No Hit
NAGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1510.12076039059188586No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1500.11996065290584688No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1460.11676170216169096No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1430.11436248910357402No Hit
NAGGANNNNGNTATGNNNGCTTCTATATGAGCGCATCTCACCTGAAGTTG1410.11276301373149608No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1410.11276301373149608No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1410.11276301373149608No Hit
NAGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGGTACCCGTGTC1400.11196327604545708No Hit
NNGGANNNNGNTATGNNNTCCTCTTCATTTTCACGGTGCCATTCCGATTC1390.11116353835941811No Hit
NNGGANNNNGNTATGNNNAAACTATTAATACCTGCAGCAAAGCCCCAAGC1380.11036380067337914No Hit
NNGGANNNNGNTATGANNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG1380.11036380067337914No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1360.10876432530130117No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1360.10876432530130117No Hit
NNGGANNNNGNTATGNNNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1350.1079645876152622No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGATTCTCAGATTCC1350.1079645876152622No Hit
NAGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1350.1079645876152622No Hit
NAGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1340.1071648499292232No Hit
NNGGANNNNGNTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1340.1071648499292232No Hit
NNGGANNNNGNTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1320.10556537455714526No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1310.1047656368711063No Hit
NNGGANNNNGNTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC1300.1039658991850673No Hit
NAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1290.10316616149902832No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1290.10316616149902832No Hit
NAGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1280.10236642381298935No Hit
NAGGANNNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1270.10156668612695036No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1270.10156668612695036No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1260.10076694844091139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)