FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70385
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT7841.113873694679264No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC7281.0343112879164595No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG6910.9817432691624636No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG5300.7530013497194005No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG5260.7473183206649144No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC5190.7373730198195638No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT5120.7274277189742132No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG4960.7046956027562691No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT4820.684805001065568No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGATTTGGGGC4730.6720181856929743No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT4500.6393407686296797No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT4490.637920011366058No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT4370.6208709242025999No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT4230.6009803225118989No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA4180.5938765361937913No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT4170.5924557789301699No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC4160.5910350216665482No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA4100.5825104780848191No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT3940.559778361866875No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA3750.5327839738580664No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG3720.5285217020672018No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC3650.5185764012218512No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT3500.49726504226752855No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG3490.4958442850039071No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG3400.48305746963131346No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT3210.4560630816225048No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG3170.4503800525680187No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT3040.43191020814093917No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG2980.42338566455921006No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT2870.40775733465937347No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG2850.4049158201321304No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA2800.39781203381402286No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA2730.3878667329686723No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2720.3864459757050508No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC2700.3836044611778078No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT2670.37934218938694325No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC2650.37650067485970024No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGCAGATCCCAGAG2640.3750799175960787No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA2590.36797613127797113No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG2550.36229310222348515No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC2530.3594515876962421No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG2490.35376855864175605No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2450.34808552958727No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2430.345244015060027No Hit
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG2420.3438232577964055No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA2420.3438232577964055No Hit
TGTAAAANGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT2410.342402500532784No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2290.32535341336932583No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC2280.3239326561057044No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT2270.3225118988420828No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT2230.3168288697875968No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC2190.31114584073311075No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT2160.3068835689422462No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT2150.3054628116786247No Hit
TGTAAAACGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG2150.3054628116786247No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT2110.2997797826241386No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG2090.29693826809689566No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC2000.28415145272430203No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT2000.28415145272430203No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC1930.2742061518789515No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA1910.27136463735170846No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1900.26994388008808695No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA1890.26852312282446544No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG1860.2642608510336009No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC1790.25431555018825036No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG1760.25005327839738584No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC1760.25005327839738584No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT1750.24863252113376427No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA1690.24010797755203522No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT1660.2358457057611707No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG1660.2358457057611707No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA1650.23442494849754922No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGCCCAGTGTGTT1600.22732116217944165No Hit
TGTAAAANGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT1600.22732116217944165No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGGTGGAGTGTGT1590.2259004049158201No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG1570.22305889038857712No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGATTTGGGGC1500.21311358954322654No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG1500.21311358954322654No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA1490.21169283227960503No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1490.21169283227960503No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA1490.21169283227960503No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1450.20600980322511897No Hit
TGTAAAANGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC1450.20600980322511897No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1440.2045890459614975No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCCTGCTACGC1420.20174753143425445No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1410.20032677417063297No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCACGAACGTG1410.20032677417063297No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT1410.20032677417063297No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCACCAACAGGCCCC1400.19890601690701143No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC1390.19748525964338992No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCACTCCAGCCTGG1390.19748525964338992No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGCTTGACACGG1390.19748525964338992No Hit
TGTAAAANGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA1380.19606450237976844No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1360.1932229878525254No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT1360.1932229878525254No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGCCCCTGCCA1350.1918022305889039No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA1330.18896071606166087No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA1310.18611920153441786No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT1300.18469844427079632No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG1280.1818569297435533No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG1250.17759465795268878No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1220.17333238616182425No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG1220.17333238616182425No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT1210.17191162889820275No Hit
TGTAAAANGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT1210.17191162889820275No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC1210.17191162889820275No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG1200.17049087163458124No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGTGCCATGTTTCC1190.1690701143709597No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT1130.16054557078923065No Hit
TGTAAAANGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT1130.16054557078923065No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA1120.15912481352560914No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGAG1120.15912481352560914No Hit
TGTAAAANGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT1110.15770405626198764No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG1110.15770405626198764No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCAGCACCCGGCAGC1100.15628329899836613No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT1090.15486254173474462No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT1070.1520210272075016No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1050.14917951268025859No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT1040.14775875541663705No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1030.14633799815301557No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT1010.14349648362577253No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGACTCAAATGTT1010.14349648362577253No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA990.14065496909852954No Hit
TGTAAAANGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT980.139234211834908No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG940.13355118278042197No Hit
TGTAAAANGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG920.13070966825317895No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG920.13070966825317895No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG910.12928891098955744No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG910.12928891098955744No Hit
TGTAAAANGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA910.12928891098955744No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG900.12786815372593593No Hit
TGTAAAANGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA900.12786815372593593No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTGCGCCACTACA900.12786815372593593No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGTGTGGCCTGA890.1264473964623144No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG880.12502663919869292No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT880.12502663919869292No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT870.1236058819350714No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGCAGATCCCAGAG860.12218512467144987No Hit
TGTAAAANGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA860.12218512467144987No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGCGAGGGGAATCA850.12076436740782837No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACACTCCCC830.11792285288058535No Hit
TGTAAAACGACGGCCAGTCGCAGAAGCAAGGGGCTGAAAAGATCTGAAGG830.11792285288058535No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCAACCCCTCAG830.11792285288058535No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG820.11650209561696384No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC810.11508133835334232No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC810.11508133835334232No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC810.11508133835334232No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC810.11508133835334232No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT790.11223982382609932No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG790.11223982382609932No Hit
TGTAAAANGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG790.11223982382609932No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT790.11223982382609932No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGATCATGCTGG770.1093983092988563No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC770.1093983092988563No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG770.1093983092988563No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGACCCTTTGCCCC770.1093983092988563No Hit
TGTAAAANGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG760.10797755203523479No Hit
TGTAAAANGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT750.10655679477161327No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG750.10655679477161327No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC750.10655679477161327No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC740.10513603750799175No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG740.10513603750799175No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA730.10371528024437025No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCGGCAGCGACAT720.10229452298074874No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTCGCCTCCTCTG720.10229452298074874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)