FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128297
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT23151.8044069619710514No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC22851.781023718403392No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC20531.6001933014801593No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA18961.477820993476075No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA16561.2907550449347998No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14681.1442200519108008No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT14231.1091451865593116No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA12390.9657279593443339No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC11360.8854454897620365No Hit
CAGGAAANAGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG10900.8495911829582922No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA10840.8449145342447602No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG10640.8293257051996539No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC9940.774764803541782No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG9100.7092917215523356No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT9030.7038356313865484No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT8830.6882468023414422No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC8220.640700873753868No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA8070.6290092519700382No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC7720.6017288011411023No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA7430.5791249990256981No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT7350.5728894674076557No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT7340.5721100259554004No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG7130.5557417554580388No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7040.548726782387741No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA6640.5175491242975284No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT6550.5105341512272306No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT6410.49962197089565613No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC5840.45519380811710325No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG5380.4193395013133589No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5040.39283849193667814No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5040.39283849193667814No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4430.345292563349104No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4410.3437336804445934No Hit
CAGGAAANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4330.3374981488265509No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT4160.32424764413821056No Hit
CAGGAAANNGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG4140.32268876123369994No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC3980.3102176979976149No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3950.30787937364084894No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG3560.2774811570028917No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3550.2767017155506364No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3540.2759222740983811No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3310.2579951206965089No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC3160.24630349891267916No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3090.240847408746892No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3020.2353913185811048No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2940.22915578696306227No Hit
CAGGAAANNGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2920.22759690405855162No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2860.22292025534501975No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2840.22136137244050913No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2800.21824360663148787No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2790.21746416517923256No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT2750.21434639937021133No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2740.213566957917956No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2710.21122863356119004No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2690.20966975065667945No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2650.20655198484765816No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2550.19875757032510505No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2550.19875757032510505No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTTGTAATCCCATC2460.1917425972548072No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2460.1917425972548072No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG2410.18784538999353065No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2410.18784538999353065No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2360.18394818273225405No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2280.17771265111421156No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG2260.17615376820970094No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2250.17537432675744563No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2230.173815443852935No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2230.173815443852935No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA2220.17303600240067968No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2200.17147711949616903No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2110.16446214642587123No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2100.1636827049736159No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1990.15510884899880745No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1980.15432940754655214No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1940.15121164173753088No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1920.14965275883302026No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1850.14419666866723305No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1840.14341722721497774No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1750.1364022541446799No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1740.13562281269242463No Hit
CAGGAAANNGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1730.1348433712401693No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1700.13250504688340337No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1700.13250504688340337No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG1660.1293872810743821No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT1640.1278283981698715No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1640.1278283981698715No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1620.12626951526536084No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1610.12549007381310553No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1600.12471063236085021No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1590.1239311909085949No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1580.12315174945633958No Hit
NAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1560.12159286655182897No Hit
NAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1530.11925454219506301No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1510.1176956592905524No Hit
CAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1510.1176956592905524No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1510.1176956592905524No Hit
CAGGAAANNGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1510.1176956592905524No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1490.11613677638604175No Hit
CAGGAAANNGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1490.11613677638604175No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1460.11379845202927581No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1450.1130190105770205No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCAGAGAGTGATAT1440.1122395691247652No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1400.10912180331574393No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1380.10756292041123332No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1350.10522459605446738No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1340.10444515460221206No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1310.10210683024544612No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1310.10210683024544612No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1290.10054794734093547No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1290.10054794734093547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)