FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105937
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC23922.2579457602159776No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA20731.9568233950366727No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC15611.4735172791376006No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG15191.4338710743177547No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG14271.3470270066171404No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC13591.2828379130992948No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC13461.270566468750295No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG12771.205433417974834No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA12631.1922180163682188No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC11561.0912145898033738No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC11271.0638398293325277No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC10971.0355211116040666No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA10791.0185298809669898No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA10560.9968188640418362No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA10310.9732199326014518No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC9920.9364055995544521No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT9720.9175264544021446No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC9630.9090308390836064No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9560.9024231382802986No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT9480.8948714802193756No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG9200.868440677006145No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG9070.8561692326571453No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9000.8495615318538376No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC8890.8391780020200684No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT8690.8202988568677609No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC8420.7948120109121459No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG7710.7277910456214542No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC7630.7202393875605313No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT7620.7192954303029159No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA7590.7164635585300697No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG7480.7060800286963006No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG7470.7051360714386852No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC7460.7041921141810699No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG7400.6985283706353775No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT7260.6853129690287624No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC7240.6834250545135316No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7150.6749294391949933No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG7070.6673777811340703No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG7050.6654898666188395No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG7040.6645459093612242No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA6950.6560502940426858No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG6790.6409469779208398No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG6530.61640408922284No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG6460.6097963884195323No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT6400.6041326448738401No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG6340.5984689013281479No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG6250.5899732860096094No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT6230.5880853714943788No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG6220.5871414142367634No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG6100.5758139271453789No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC5970.563542482796379No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5890.555990824735456No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5850.5522149957049945No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT5820.5493831239321484No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5790.5465512521593022No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5770.5446633376440715No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA5640.5323918932950716No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT5630.5314479360374562No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5610.5295600215222255No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG5560.5248402352341486No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5550.5238962779765332No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG5540.5229523207189178No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5400.5097369191123027No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG5400.5097369191123027No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA5360.5059610900818411No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC5030.4748105005805337No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT5000.4719786288076876No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4850.45781926994345695No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT4730.44649178285207247No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA4710.4446038683368417No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT4690.44271595382161094No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA4660.43988408204876484No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT4570.4313884667302264No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC4560.4304445094726111No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT4560.4304445094726111No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA4560.4304445094726111No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT4460.4210049368964573No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4420.4172291078659959No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC4160.39268621916799606No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4030.3804147748189962No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4020.3794708175613808No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3970.37475103127330395No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG3800.35870375789384257No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT3590.3388806554839197No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC3230.30489819420976616No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA3180.3001784079216893No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3150.2973465361488432No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT2990.2822432200269972No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG2840.26808386116276656No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2810.2652519893899204No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG2790.26336407487468966No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2770.2614761603594589No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG2740.25864428858661276No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC2710.25581241681376665No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG2680.25298054504092055No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2460.23221348537338227No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG2400.22654974182769005No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2370.2237178700548439No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG2330.21994204102438242No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG2120.20011893861445954No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG2120.20011893861445954No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2050.19351123781115193No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG1910.18029583620453668No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGTTGGGGTCCC1790.16896834911315214No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1700.1604727337946138No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG1680.15858481927938303No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1620.1529210757336908No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGTCA1620.1529210757336908No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTTAGCTA1610.1519771184760754No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC1600.15103316121846003No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC1570.1482012894456139No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1570.1482012894456139No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGCCGCCCGCTGC1410.1330979733237679No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGGGTGCTTTCTCT1330.1255463152628449No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC1310.12365840074761414No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC1300.12271444348999877No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC1280.12082652897476803No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC1260.11893861445953727No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT1250.1179946572019219No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1240.11705069994430652No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGAACCTGGGA1200.11327487091384503No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACACCTTC1200.11327487091384503No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1180.11138695639861428No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA1180.11138695639861428No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1150.10855508462576813No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC1120.10572321285292202No Hit
TGTAAAACGACGGCCAGTTTACAGGTGTGAGCCACCGCGCCCGGCTGGGA1080.1019473838224605No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG1080.1019473838224605No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC1080.1019473838224605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)