FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142448
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC87446.138380321240032No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG49713.4896944850050544No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC47473.3324441199595642No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC43343.0425137594069414No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG39012.738543187689543No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG35792.5124957879366505No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC29562.0751432101538807No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG29162.047062787824329No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA19391.3611984724250252No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG18931.3289059867460407No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA18721.314163765023026No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC17211.2081601707289678No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC16541.1611254633269685No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT15151.0635459957317759No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG14591.0242334044704033No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC14240.9996630349320454No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13210.9273559474334494No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC13170.9245479052004943No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT12990.9119117151521959No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT12870.9034875884533303No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC12610.8852353139391216No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12120.8508367965854207No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA11690.8206503425811523No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC11400.8002920363922272No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC10430.7321970122430642No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA9570.6718241042345278No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC9210.646551724137931No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT7980.5602044254745592No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG7610.5342300348197238No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC7440.5222958553296642No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7260.5096596652813659No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG7160.5026395596989779No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT7120.4998315174660227No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT6940.48719532741772437No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG6840.48017522183533645No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG6790.4766651690441424No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG6340.4450746939233966No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA5550.3896158598225317No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5390.378383690890711No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA5040.35381332135235316No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA4780.33556104683814447No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG4710.3306469729304729No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT4660.32713692013927886No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC4600.3229248567898461No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA4380.3074806245085926No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4340.3046725822756374No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC4290.30116252948444344No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4230.29695046613501064No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4180.2934404133438167No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTCACT4140.29063237111086154No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC4120.2892283499943839No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT3810.2674660226889813No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC3500.24570369538357856No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT3310.23236549477704144No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT3270.22955745254408627No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT3210.22534538919465347No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC3190.2239413680781759No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT3180.2232393575199371No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2930.2056890935639672No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2910.2042850724474896No Hit
TGTAAAACGACGGCTAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC2870.20147703021453442No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT2760.19375491407390766No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGAG2700.1895428507244749No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2690.1888408401662361No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTAGCTCACT2660.18673480849151972No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG2560.17971470290913175No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT2540.17831068179265416No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGAATCCCCAAGCCCCA2250.15795237560372907No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT2210.1551443333707739No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGCGGTCATA2160.15163428057957992No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2020.14180613276423676No Hit
TGTAAAACGACGGCTAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC1860.13057396383241604No Hit
TGTAAAACGACGGCTAGTTGGAACCATTTTTGATAACTTTCTGATCACAG1730.1214478265753117No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGACAACTTTCTGATCACAG1720.12074581601707289No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA1640.11512973155116252No Hit
TGNAAAACGACGGCCAGTTGGAACCATTTTTGATAACCTTCTGATCACAG1640.11512973155116252No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1590.11161967875996855No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC1580.11091766820172975No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACACCCTGGAGGAGATGAAC1550.10881163652701337No Hit
TGNAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCCGCGGGGGTACCG1520.10670560485229698No Hit
TGTAAAACGACGGCTAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC1490.10459957317758059No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA1480.1038975626193418No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)