FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142448
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC25081.7606424800629004No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC23781.6693811074918568No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA22391.5718016398966639No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA21791.5296810064023363No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC17711.2432606986409074No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT16241.1400651465798046No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT15191.066354037964731No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA14921.0473997528922836No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG14891.0452937212175673No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT14821.0403796473098956No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA12510.8782152083567337No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC10860.7623834662473324No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA9970.6999045265640795No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA9490.6662080197686173No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA9380.6584859036279905No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC9330.6549758508367965No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT8930.6268954285072448No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT8180.574244636639335No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC7520.5279119397955745No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA7320.5138717286307987No Hit
CAGGAAANAGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT7290.5117656969560822No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6970.4893013590924407No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG6680.4689430529035156No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA6140.43103448275862066No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5840.40997416601145686No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG5790.4064641132202628No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT5610.3938279231719645No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5610.3938279231719645No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5490.3854037964730989No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC5480.38470178591486015No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5430.3811917331236662No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC5340.374873638099517No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5290.37136358530832303No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT5150.3615354374929799No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4830.3390710996293384No Hit
CAGGAAANAGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA4740.33275300460518925No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT4490.3152027406492194No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT4230.29695046613501064No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4100.2878243288779063No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA4040.2836122655284735No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3800.2667640121307424No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3700.2597439065483545No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG3510.24640570594181735No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3470.24359766370886218No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3400.23868358980119062No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3140.2204313152869819No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG3090.21692126249578794No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3020.21200718858811637No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2960.2077951252386836No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2950.20709311468044478No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2920.20498708300572843No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2900.20358306188925082No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2900.20358306188925082No Hit
CAGGAAANNGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT2870.20147703021453442No Hit
CAGGAAANNGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2840.19937099853981805No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2780.19515893519038527No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2700.1895428507244749No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2680.1881388296079973No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2630.1846287768168033No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2600.18252274514208694No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2550.17901269235089295No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2460.1726945973267438No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2420.1698865550937886No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC2420.1698865550937886No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2410.16918454453554982No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA2400.16848253397731103No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2380.16707851286083342No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2360.16567449174435583No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2300.16146242839492306No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2260.15865438616196786No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2240.15725036504549028No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2180.1530383016960575No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2180.1530383016960575No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2170.1523362911378187No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2110.14812422778838594No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2110.14812422778838594No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2100.14742221723014715No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2100.14742221723014715No Hit
CAGGAAANNGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2090.14672020667190835No Hit
CAGGAAANNGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2080.14601819611366953No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2080.14601819611366953No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2070.14531618555543077No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2020.14180613276423676No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2020.14180613276423676No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2010.141104122205998No Hit
CAGGAAANNGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2000.14040211164775918No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1900.1333820060653712No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTTGTAATCCCATC1900.1333820060653712No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1890.13267999550713244No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1860.13057396383241604No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1850.12987195327417725No Hit
CAGGAAANNGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1840.12916994271593846No Hit
CAGGAAANNGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA1820.12776592159946085No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1820.12776592159946085No Hit
NAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1810.12706391104122206No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1800.12636190048298326No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT1790.12565988992474447No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1790.12565988992474447No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1780.12495787936650568No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1770.12425586880826688No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1760.12355385825002808No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1760.12355385825002808No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT1750.12285184769178928No Hit
NAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1740.12214983713355047No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC1680.11793777378411771No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1660.11653375266764013No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1630.11442772099292374No Hit
CAGGAANNNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1630.11442772099292374No Hit
CAGGAAACAGCTATGANCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1620.11372571043468493No Hit
CAGGAAACNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1620.11372571043468493No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGCTTGAGCCCAG1610.11302369987644614No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1600.11232168931820735No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1590.11161967875996855No Hit
CAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1590.11161967875996855No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1580.11091766820172975No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1580.11091766820172975No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1580.11091766820172975No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1540.10810962596877456No Hit
NAGGANANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1540.10810962596877456No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT1520.10670560485229698No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1520.10670560485229698No Hit
CAGGAANNNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1490.10459957317758059No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1490.10459957317758059No Hit
CAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1480.1038975626193418No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1470.103195552061103No Hit
CAGGAAACNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1460.10249354150286422No Hit
CAGGANANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1460.10249354150286422No Hit
CAGGAAANNGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1450.10179153094462541No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1450.10179153094462541No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1450.10179153094462541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)