FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113447
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT45343.9965799007466036No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC45123.977187585392298No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT41103.622837095736335No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT32082.827752166209772No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC30872.7210944317610863No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT29712.6188440417111076No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT28122.4786904898322564No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC27952.463705518876656No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG27012.3808474441809833No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC25942.2865302740486744No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG22932.021208141246573No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG22441.9780161661392546No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA22371.971845883981066No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT17941.581355170255714No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG16171.4253351785415216No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT16151.423572240782039No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA15531.3689211702380848No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG15341.352173261523002No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT15321.3504103237635194No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT14491.2772484067449998No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG13291.1714721411760558No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT12911.1379763237458902No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT11661.02779271377824No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG10620.9361199502851553No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA10480.9237793859687784No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG10370.9140832282916251No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA9720.8567877511084472No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT9430.8312251535959523No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC8970.790677585127857No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA8970.790677585127857No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC8730.7695223320140683No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG7600.6699163486033125No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA7240.6381834689326293No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG6900.6082135270214285No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT6760.5958729627050516No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC5770.5086075436106728No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA5600.49362257265507237No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG5370.4733487884210248No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG5350.4715858506615424No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG5350.4715858506615424No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA5320.4689414440223188No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA4910.4328012199529296No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG4800.4231050622757764No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC4580.4037127469214699No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA4480.3948980581240579No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC4300.3790316182887163No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC4270.37638721164949274No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA3980.3508246141369979No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT3760.3314322987826915No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC3560.31380292118786746No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3370.29705501247278465No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA3330.2935291369538198No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT3230.28471444815640784No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3070.27061094608054864No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC3010.26532213280210143No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC2990.263559195042619No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC2310.20361931122021737No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGCAGAAGGG1830.16130880499263975No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAATGGGGTTCAAC1640.14456089627755692No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTA1560.13750914523962732No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA1500.1322203319611801No Hit
CAGGAAACAGCTATGACCTGGAGCCAATTTCATGAGGGCAGACAGCACGT1480.13045739420169772No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTCCCCGCCCCTC1460.1286944564422153No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCGGGCAGAG1340.11811682988532089No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGGGATGAGGT1330.1172353610055797No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC1320.11635389212583849No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1290.1137094854866149No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCTGAGATCAT1200.1057762655689441No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTTTCACATCTTG1200.1057762655689441No Hit
CAGGAAACAGCTATGACCTGGAGCCAATTTCATGAGGGCAGACAGCATGT1190.10489479668920289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)