FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206285
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT38071.8455050052112367No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT37271.806723707492062No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT36501.769396708437356No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT23251.1270814649635215No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC23081.1188404391981968No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC21361.0354606491019707No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC20050.9719562740868217No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG19190.9302663790387087No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT18580.9006956395278378No Hit
CAGGAAANAGCTATGANNCCCCTCCGCTGGTTGAATGGCAGGAAACAGCT18540.8987565746418789No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT18140.8793659257822914No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT18100.8774268608963328No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT16560.802772862786921No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA16230.7867755774777614No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT15040.7290883971204887No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT14920.7232712024626123No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT14810.7179387740262259No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA11710.567661245364423No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA11290.5473010640618562No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC10410.5046416365707638No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10390.5036721041277844No Hit
CAGGAAANAGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT9750.47264706595244443No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC9730.471677533509465No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC9650.4677994037375476No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT8970.4348353006762489No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8950.4338657682332695No Hit
CAGGAAANAGCTATGANNCCCCTCCGCTGTCTATGCCCCGAAGCAACTGG8620.4178684829241099No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT8420.4081731584943161No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC8420.4081731584943161No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC7890.38248054875536275No Hit
CAGGAAANNGCTATGANNCCCCTCCGCTGGTTGAATGGCAGGAAACAGCT7600.3684223283321618No Hit
CAGGAAANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC7510.36405943233875465No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA7230.3504859781370434No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG7170.3475773808081053No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA7020.34030588748576No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6980.3383668225998013No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA6580.31897617374021375No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT6520.31606757641127564No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT6510.31558281018978596No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6380.30928084931042005No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6170.2991007586591366No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6040.2927987977797707No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT5950.2884359017863635No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5760.2792253435780595No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC5200.252078435174637No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT5080.24626124051676082No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4900.23753544852994643No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4840.23462685120100832No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4720.22880965654313207No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4520.21911433211333833No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4470.21669050100588994No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA4420.21426666989844148No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC4310.20893424146205494No Hit
CAGGAAANNGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT4290.20796470901907554No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4290.20796470901907554No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4250.2060256441331168No Hit
CAGGANANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4220.20457134546864775No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA4180.202632280582689No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4170.2021475143611993No Hit
NAGGANANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4120.1997236832537509No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4040.19584555348183338No Hit
NAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4000.19390648859587464No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGTT3990.19342172237438496No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3940.1909978912669365No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC3930.19051312504544685No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3890.18857406015948808No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3830.18566546283054997No Hit
CAGGAAANNGCTATGANNCCCCTCCGCTGTCTATGCCCCGAAGCAACTGG3760.18227209928012217No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3740.1813025668371428No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3550.17209200862883875No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC3490.16918341129990064No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC3450.16724434641394187No Hit
CAGGAAANAGCTATGANNTGCTTCAGTCGCCTGCACAAGGGGACATGGCC3410.16530528152798313No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3270.15851855442712753No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCCTGGGTATGTGG3240.15706425576265845No Hit
CAGGAAANAGCTATGANNCTGGGCCCCAAAGCAAAGGAGTAGGTGACGAG3200.1551251908766997No Hit
CAGGAAANAGCTATGANNGCAGCCACCCTGAACACACTTGGCTATCACCC3060.14833846377584411No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2970.14397556778243692No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2950.14300603533945755No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGTACC2900.14058220423200912No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2870.13912790556754007No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2850.1381583731245607No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2850.1381583731245607No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2840.13767360690307098No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC2810.13621930823860193No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2790.13524977579562256No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2750.13331071090966382No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2660.12894781491625665No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2600.1260392175873185No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2560.12410015270135977No Hit
CAGGAAACAGCTATGANCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2510.12167632159391134No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2500.12119155537242164No Hit
CAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2480.12022202292944228No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2450.11876772426497322No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2430.11779819182199384No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2410.11682865937901449No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2400.11634389315752479No Hit
NAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2340.11343529582858666No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2340.11343529582858666No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2320.11246576338560729No Hit
CAGGAANNNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2320.11246576338560729No Hit
NAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2260.10955716605666917No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2260.10955716605666917No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2250.1090723998351795No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2200.10664856872773104No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2200.10664856872773104No Hit
NAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2200.10664856872773104No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2190.10616380250624137No Hit
NAGGAAANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2170.105194270063262No Hit
CAGGAAACNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2170.105194270063262No Hit
NAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2160.1047095038417723No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2150.10422473762028261No Hit
NAGGANANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2130.10325520517730324No Hit
CAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2120.10277043895581357No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2100.10180090651283417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)