FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129559
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8360.6452658634290169No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT7420.5727120462491992No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7020.54183808149183No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC6370.49166788876110495No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5960.4600220748848015No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5770.4453569416250512No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5400.41679852422448455No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC5250.40522078744047113No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4990.3851527103481811No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4820.3720312753262992No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4790.36971572796949653No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC4730.36508463325589113No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4690.3619972367801542No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT4630.3573661420665488No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4240.32726402642811386No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4100.3164581387630346No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA3950.30488040197902117No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3870.2987056090275473No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3830.29561821255181037No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3750.28944341960033654No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3710.2863560231245996No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3690.28481232488673114No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3600.27786568281632307No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3500.27014719162698075No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3440.2655160969133754No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3430.2647442477944411No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC3410.2632005495565727No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG3380.26088500219977No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3320.2562539074861646No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3280.25316651101042764No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3250.25085096365362497No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3190.2462198689400196No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3070.23695767951280883No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA3040.23464213215600616No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2780.21457405506371616No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2750.21225850770691346No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2750.21225850770691346No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2720.20994296035011076No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2550.19682152532822886No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2540.1960496762092946No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2530.1952778270903604No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2430.1875593359010181No Hit
NNGGANNNNGNTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2420.18678748678208384No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2410.1860156376631496No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG2360.18215639206847845No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2350.1813845429495442No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2350.1813845429495442No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC2330.17984084471167577No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2310.17829714647380732No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT2300.17752529735487307No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2290.17675344823593883No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC2270.17520974999807037No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2250.17366605176020192No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT2190.16903495704659655No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2160.16671940968979385No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT2140.16517571145192536No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2130.16440386233299115No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2130.16440386233299115No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2090.16131646585725423No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2070.15977276761938575No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2020.15591352202471462No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2000.15436982378684616No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1960.15128242731110922No Hit
NNGGANNNNGNTATGNNNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1960.15128242731110922No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1910.1474231817164381No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1860.1435639361217669No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1790.1381609922892273No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1790.1381609922892273No Hit
NAGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1790.1381609922892273No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1770.13661729405135883No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1770.13661729405135883No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1770.13661729405135883No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1750.13507359581349038No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1740.13430174669455613No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1720.1327580484566877No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1710.13198619933775346No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1690.130442501099885No Hit
NAGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1690.130442501099885No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1680.12967065198095076No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1670.12889880286201652No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1660.1281269537430823No Hit
NAGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1650.12735510462414806No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1650.12735510462414806No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCGGGGATCCTCG1640.12658325550521382No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1620.1250395572673454No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1620.1250395572673454No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1610.12426770814841115No Hit
NAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1590.12272400991054269No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1570.12118031167267422No Hit
NAGGAAANAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1550.11963661343480575No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1550.11963661343480575No Hit
NAGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1530.1180929151969373No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1520.11732106607800308No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1500.1157773678401346No Hit
NAGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1490.11500551872120038No Hit
NAGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1460.11268997136439768No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1450.11191812224546345No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1450.11191812224546345No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1440.11114627312652922No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1430.110374424007595No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1430.110374424007595No Hit
NAGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1420.10960257488866076No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT1410.10883072576972654No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCACACAGGA1410.10883072576972654No Hit
NNGGANNNNGNTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1400.10805887665079231No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1370.10574332929398961No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1360.10497148017505538No Hit
NNGGANNNNGNTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1360.10497148017505538No Hit
NNGGAAANNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1350.10419963105612114No Hit
NAGGAANNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1350.10419963105612114No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1340.10342778193718694No Hit
NAGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1340.10342778193718694No Hit
NAGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1330.1026559328182527No Hit
NAGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1330.1026559328182527No Hit
NAGGANNNAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1320.10188408369931846No Hit
NAGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1310.10111223458038424No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG1300.10034038546145No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1300.10034038546145No Hit
NAGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1300.10034038546145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)