FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127504
Sequences flagged as poor quality0
Sequence length300
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT61974.8602396787551765No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG39783.1199021207177813No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC32262.530116702221107No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT28822.260321244823692No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT28632.2454197515372067No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC23421.8368051198393778No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG22231.7434747145187601No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT20021.5701468189233279No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT19421.5230894717028485No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT19341.516815158740118No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT19181.5042665328146567No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG18461.4477977161500815No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG17161.3458401305057095No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT15861.2438825448613375No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC15811.239961099259631No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG15011.1772179696323253No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT13821.0838875643117079No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT13661.0713389383862466No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC12881.0101643869996235No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG12801.003890074036893No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC12000.9411469444095871No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCAGGGCAGAAGGG11510.9027167775128624No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG11490.9011481992721797No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC11240.8815409712636467No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA11050.8666394779771615No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG10940.8580122976534069No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT10700.8391893587652152No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG10630.833699334922826No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG10500.8235035763583887No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT10460.8203664198770234No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA10230.802327770109173No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC10090.7913477224243946No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT9880.7748776508972268No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA9000.7058602083071903No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAAGCAAC8510.6674300414104656No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA8490.6658614631697829No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA8390.6580185719663697No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG8170.6407642113188606No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG8030.6297841636340821No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG7600.5960597314594052No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG7550.5921382858576986No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC7500.588216840255992No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC7280.5709624796084829No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA6780.5317480235914167No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG6690.5246894215083449No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT6330.49645501317605717No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT6330.49645501317605717No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG6300.4941021458150332No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGGCAGAAGGG6190.4854749654912787No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCACTTCCTGC6160.48312209813025475No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG5870.46037771364035635No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC5840.4580248462793324No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAGCCTCC5670.44469193123352996No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC5600.4392019073911407No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT5290.41488894466055964No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG5240.41096749905885305No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCCTAAGGGAG5160.4046931860961224No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGTGCCATGTTTCC4980.39057598192997867No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA4980.39057598192997867No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA4900.3843016689672481No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT4840.37959593424520016No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTCCCCCTGCTG4800.37645877776383485No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT4620.36234157359769104No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC4600.3607729953570084No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG4560.3576358388756431No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGATGGCACTGA4480.35136152591291253No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG4320.33881289998745134No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGAGGCTCCTGGGGA4170.32704856318233155No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGTGGGAGACCCAGC4050.3176370937382357No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC3940.3090099134144811No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACTGGAGTGCA3790.2972455766093613No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC3750.294108420127996No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCAGCACGACG3700.29018697452628933No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC3640.28548123980424145No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTGCTGCCTCT3460.27136403563809763No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGTGGGAGA3440.26979545739741495No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT3410.267442590036391No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG3300.2588154097126365No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG3290.25803112059229516No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA3190.2501882293888819No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGGTGACAGAGTGTG3090.24234533818546872No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCATTGGTGGTAGCT3000.23528673610239678No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCATAGCTGTT2950.23136529050069016No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTACGGCACTGGT2910.22822813401932487No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCAGTGCCTTGCCAG2850.22352239929727694No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT2740.2148952189735224No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG2700.21175806249215712No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCCTGCTGTCCTC2680.21018948425147446No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT2620.20548374952942652No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC2600.20391517128874387No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC2580.2023465930480612No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2520.1976408583260133No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC2440.1913665453632827No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2430.1905822562429414No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCCGGAGTGCAATG2430.1905822562429414No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCATCCTCCTCTTC2410.18901367800225874No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACACTCCCC2380.18666081064123477No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGGCAGGAGAG2330.18273936503952817No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG2310.18117078679884552No Hit
TGTAAAACGACGGCCAGTCGCAGAAGCAAGGGGCTGAAGTCCGTCTTTGG2220.17411218471577364No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTACGGCCAGTACC2200.17254360647509098No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA2190.17175931735474967No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGAATGG2130.1670535826327017No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGACTCCAAGTGG2100.16470071527167776No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGGCTGGAGTGC2040.15999498054962982No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT1940.1521520893462166No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT1900.1490149328648513No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCTAGG1870.14666206550382732No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA1820.14274061990212072No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC1730.13568201781904882No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1710.13411343957836616No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGACCCACGAGGGCT1680.13176057221734222No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGCAGATCCCAGAG1660.13019199397665956No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTGGCTCCTGGG1650.12940770485631825No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG1550.12156481365290502No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCTAGCACGACG1500.11764336805119839No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA1480.11607478981051575No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACCGCCGAGTG1480.11607478981051575No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGTGGGGTTAG1360.10666332036641987No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG1330.1043104530053959No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG1320.10352616388505458No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACTCCGTTGCC1300.10195758564437193No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCACATGTACTCT1290.1011732965240306No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGCTGTTTCCTGA1290.1011732965240306No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)