FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140981
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA39612.8095984565296033No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG30862.188947446819075No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG29922.1222717954901724No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG29712.1073761712571195No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA26941.9108957944687581No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA26431.8747207070456302No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC25861.8342897269844873No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG25361.7988239550010283No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC24901.7661954447762462No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG22611.6037622090920054No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT20341.4427476042871026No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA19461.3803278455962151No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT19201.3618856441648164No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC19111.3555018052077938No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC17311.2278250260673425No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC16401.1632773210574476No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA16121.1434164887467104No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT15251.0817060454954923No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT14311.0150303941665897No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC14211.007937239769898No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCGGAGGGGGGTCA14020.9944602464161838No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT13950.9894950383384995No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC13260.9405522730013265No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13120.930621856845958No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT12980.9206914406905895No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT12240.8682020981550705No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC12160.8625275746377172No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG12130.8603996283187096No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG12090.8575623665600329No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT10550.74832778885098No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT10250.7270483256609047No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT9890.7015129698328144No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA9760.692291869117115No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC9680.6866173455997617No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC9460.6710124059270398No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT9420.6681751441683631No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT9290.6589540434526638No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC9150.6490236272972955No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT8740.6199416942708592No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA8710.6178137479518516No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT8670.6149764861931749No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT8620.6114299089948291No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG8210.5823479759683929No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG8080.5731268752526936No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC7680.5447542576659266No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG7590.538370418708904No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7410.5256027407948589No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT7380.5234747944758513No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT7360.522056163596513No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT7060.5007767004064377No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7060.5007767004064377No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA6750.4787879217766933No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG6740.47807860633702415No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC6580.4667295593023173No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA6520.4624736666643023No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG6490.46034572034529475No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG6400.4539618813882722No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT5930.42062405572382094No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5920.41991474028415177No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5830.4135309013271292No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG5690.4036004851717607No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG5400.38303033742135467No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT5390.3823210219816855No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG5320.37735581390400125No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC4920.34898319631723423No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA4810.3411807264808733No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA4710.33408757208418155No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4500.31919194785112887No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT4310.30571495449741454No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT4270.3028776927387379No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT4210.2986218001007228No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT4010.2844354913073393No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA3930.2787609677899859No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA3920.2780516523503167No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT3830.27166781339329416No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT3830.27166781339329416No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA3820.270958497953625No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC3810.2702491825139558No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCCGCCTCC3710.263156028117264No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3560.2525162965222264No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT3380.23974861860818125No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA3360.23832998772884292No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3120.22130641717678268No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG3080.218469155418106No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3000.2127946319007526No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGAGGGTCATAGCT2800.19860832310736906No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG2780.1971896922280307No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGTCTGGTAGTAG2720.19293379959001566No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2660.18867790695200062No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2630.1865499606329931No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2550.1808754371156397No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC2520.17874749079663219No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2520.17874749079663219No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC2520.17874749079663219No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT2480.17591022903795547No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGACAGATGCCCC2460.17449159815861712No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT2420.17165433639994043No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT2420.17165433639994043No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCGGCCTCC2410.17094502096027125No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG2300.1631425511239103No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2270.1610146048049028No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCCAAGAGGTGGG2270.1610146048049028No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAGTTCTGAA2240.15888665848589528No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2240.15888665848589528No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG2190.1553400812875494No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG2090.14824692689085764No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCTGTCATCTCT2040.14470034969251175No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCACTTCCTGC2010.14257240337350424No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGAGAAGGGGC2000.14186308793383506No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1980.1404444570544967No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC1950.13831651073548917No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1890.13406061809747413No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCACGTGTCGCCC1890.13406061809747413No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1880.13335130265780495No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1820.1290954100197899No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGGGGCGAGGG1810.12838609458012074No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1810.12838609458012074No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC1800.12767677914045156No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1770.12554883282144402No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1770.12554883282144402No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT1680.11916499386442146No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG1670.11845567842475227No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1640.11632773210574475No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCTGCTCACT1520.10781594682971465No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT1510.10710663139004548No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1470.10426936963136876No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTGTAAAACGACG1460.10356005419169959No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGTACTGGCAGAG1460.10356005419169959No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCCAGTGCCTCCG1440.10214142331236124No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG1410.10001347699335372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)