FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170045
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA13840.8139022023581993No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA11070.6510041459613631No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT9960.5857273074774324No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT9140.5375047781469611No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT7890.46399482489929134No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC7560.44458819724190657No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA7170.42165309182863364No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6990.41106765856096916No Hit
NNGGANNNNGNTATGNNNTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA6950.40871534005704374No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC6270.36872592549031136No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA6240.36696168661236733No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT6010.35343585521479604No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT5740.3375577053132994No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC5680.33402922755741127No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5670.33344114793142987No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5510.3240318739157282No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5250.30874180364021286No Hit
NNGGANNNNGNTATGANNTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA5230.3075656443882502No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA5140.30227292775441794No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT5100.2999206092504925No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4960.2916874944867535No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4920.2893351759828281No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4440.2611073539357229No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC4370.2569907965538534No Hit
NNGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC4250.2499338410420771No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4200.2469934429121703No Hit
NAGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT4190.24640536328618895No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4080.23993648740039403No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4070.23934840777441266No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA4060.23876032814843132No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4030.2369960892704872No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3860.22699873562880413No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3580.21053250610132612No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3580.21053250610132612No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3550.20876826722338201No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3480.20465170984151254No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3420.20112323208562438No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT3350.19700667470375488No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3350.19700667470375488No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3320.19524243582581083No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT3270.19230203769590404No Hit
NAGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT3260.19171395806992267No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC3240.19053779881795996No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3210.18877355994001588No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG3080.18112852480225822No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3010.17701196742038872No Hit
NNGGANNNNGNTATGNNNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG2990.175835808168426No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2940.17289541003851921No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2930.17230733041253787No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2920.17171925078655648No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2870.1687788526566497No Hit
NAGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC2860.16819077303066834No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2860.16819077303066834No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2830.16642653415272426No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2820.16583845452674292No Hit
NNGGANNNNGNTATGANNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT2770.16289805639683613No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT2680.15760533976300392No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2660.15642918051104118No Hit
NAGGANNNNGNTATGNNNTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA2650.15584110088505984No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT2650.15584110088505984No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2560.15054838425122763No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2550.14996030462524626No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2520.14819606574730218No Hit
NAGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2410.14172718986150723No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC2400.1411391102355259No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2400.1411391102355259No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2380.13996295098356318No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2360.13878679173160044No Hit
NNGGANNNNGNTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2350.1381987121056191No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2340.13761063247963776No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT2320.13643447322767502No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2270.13349407509776823No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2260.1329059954717869No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2250.13231791584580552No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT2240.13172983621982415No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT2230.1311417565938428No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2210.1299655973418801No Hit
NAGGAAANAGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2200.12937751771589873No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2190.12878943808991736No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2190.12878943808991736No Hit
NAGGAANNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2150.12643711958599194No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2120.12467288070804787No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2120.12467288070804787No Hit
NAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2120.12467288070804787No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA2110.1240848010820665No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC2080.12232056220412243No Hit
NAGGANNNNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2050.12055632332617836No Hit
NNGGANNNNGNTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2040.11996824370019701No Hit
NAGGANNNAGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2030.11938016407421566No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2000.11761592519627159No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1990.11702784557029021No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1990.11702784557029021No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1970.11585168631832748No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1950.11467552706636479No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1920.11291128818842072No Hit
NNGGANNNNGNTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1920.11291128818842072No Hit
NNGGAAANNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1910.11232320856243935No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1910.11232320856243935No Hit
NAGGANNNNGNTATGANNTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA1890.11114704931047664No Hit
NAGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1860.10938281043253258No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGATACTACTA1830.10761857155458848No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1820.10703049192860713No Hit
NNGGAAANNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1810.10644241230262579No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1810.10644241230262579No Hit
NNGGANNNNGNTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1810.10644241230262579No Hit
NNGGANNNNGNTATGANNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1800.1058543326766444No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1780.1046781734246817No Hit
NAGGAANNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1770.10409009379870034No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1770.10409009379870034No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT1720.10114969566879356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)