FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132334
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT38512.910060906494174No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC32292.440038085450451No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC26441.997974821285535No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA25501.9269424335393777No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC25311.912584823250261No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT25221.9057838499554158No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT23901.8060362416310247No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT22681.7138452703009053No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC21091.5936947420919796No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT19201.4508743029002373No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT19141.446340320703674No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT17721.3390360753850106No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT17611.3307237746913114No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA17561.3269454561941754No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG15671.1841250170024333No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT15561.175812716308734No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC15441.1667447519156076No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT14801.1183822751522663No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT14701.1108256381579942No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG14571.1010020100654405No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC13821.0443272326084No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA13321.0065440476370395No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT11990.906040775613221No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT11780.8901718379252497No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA11650.8803482098326961No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA10820.8176281227802378No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC10590.8002478576934121No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT10020.7571750268260613No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC9530.7201475055541282No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA9230.697477594571312No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT9210.6959662671724576No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT8870.6702737013919325No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC8810.6657397191953693No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG8580.6483594541085436No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG8550.6460924630102619No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC8520.6438254719119803No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC8390.6340018438194266No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCACAGGAAACAG7810.5901733492526486No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC7710.5826167122583765No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC7670.5795940574606677No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA7610.5750600752641045No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT7610.5750600752641045No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA7330.5539014916801427No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA7160.5410552087898802No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA6890.5206522889053455No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT6870.5191409615064911No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC6840.5168739704082095No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT6710.5070503423156558No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTCAGGAAACAGCT6700.5062946786162286No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC6610.49949370532138376No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC6550.49495972312482056No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG6440.4866474224311213No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG6310.4768237943385676No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG6040.45642087445403295No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC5940.4488642374597609No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT5810.43904060936720724No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGATCTGGGGACT5590.42241600797980866No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA5260.3974791058987109No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA5240.3959677784998564No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT5200.39294512370214757No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA4770.36045158462677773No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT4540.3430713195399519No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG4460.3370260099445343No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTCTGTCACCCCAT4340.3279580455514078No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4280.3234240633548446No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC3810.28790786948176583No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG3730.2818625598863482No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGACTGGAAACTGG3610.2727945954932217No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCATATTCCTCTA3580.2705276043949401No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCCAGCTGGA3560.26901627699608566No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3450.2607039763023864No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC3320.2508803482098327No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGT3260.24634636601326945No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG3170.23954539271842462No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3150.2380340653195702No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC3070.2319887557241525No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA3000.22669910982816205No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2990.22594344612873488No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC2940.22216512763159882No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC2860.2161198180361812No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2850.215364154336754No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA2590.19571689815164658No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2580.19496123445221938No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT2550.19269424335393776No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCCGT2530.19118291595508335No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACAGGAAACAGCT2500.18891592485680173No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT2450.1851376063596657No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC2330.1760696419665392No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCAGGAAACAGCT2310.1745583145676848No Hit
CAGGAAACAGCTATGACCGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG2300.17380265086825758No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT2120.16020070427856786No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC2100.15868937687971346No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCAGCAGCCACAGG1910.14433176659059654No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTCACATCTATAAT1830.13828645699517886No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1780.13450813849804283No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC1650.12468451040548914No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCTGGGAGGTGGG1600.12090619190835311No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCTGGGCTGGT1580.1193948645094987No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTGGCCTGAGCCAC1530.11561654601236265No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCAGCACAGGGAG1480.11183822751522662No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCCGTCGTTTTAC1430.1080599090180906No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT1360.10277026312210014No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCTTGAGCCCAG1360.10277026312210014No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1350.10201459942267292No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCCCCCAACTTCAC1340.10125893572324574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)