FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186622
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG50042.6813558958750843No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG45852.456837886208486No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT44482.383427463000075No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT40942.1937392161695835No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA37261.9965491742666996No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA37001.9826172691322568No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC34681.8583018079326126No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA33291.7838196997138602No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT31621.6943340013503232No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC30921.6568250259883615No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG26711.431235331311421No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT25681.3760435532788202No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC25571.3701492857219406No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC24481.3117424526583146No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC22591.2104682191810183No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC22491.2051097941293096No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG22351.1976079990569173No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA21581.1563481261587594No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT20241.0845452304658614No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT18190.9746975169058311No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG17840.9559430292248502No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA16970.9093247312749837No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG16680.8937852986250281No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT16350.8761024959543892No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT15900.8519895832216995No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA15270.8182315053959339No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG15230.8160881353752505No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG15110.8096580253131999No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACGTGAG13550.7260665945065427No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG13400.7180289569289794No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13180.7062404218152201No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC13110.7024895242790239No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13070.7003461542583405No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT12870.6896293041549229No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTGTGCAGGCG12790.6853425641135558No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAGGAATTCAG12400.6644447064118915No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC12280.6580145963498408No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC11710.6274715735551006No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC11580.6205056209878793No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT11460.6140755109258287No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT11350.6081812433689491No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT11150.5974643932655314No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT11050.5921059682138227No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG10990.5888909131827973No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG10780.5776382205742089No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC10240.5487027252949813No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA10160.5444159852536143No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC9980.5347708201605383No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG9950.5331632926450258No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT9890.5299482376140005No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA9390.5031561123554564No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC9350.501012742334773No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC9250.4956543172830642No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG9130.4892242072210136No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCTTCGTGGAGTG8680.465111294488324No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG8660.4640396094779823No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC8570.4592170269314443No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG8520.45653781440558994No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG8500.4554661293952481No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC8490.45493028689007725No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT8360.4479643343228559No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG8290.4442134367866597No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC8010.429209846641875No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT7910.42385142159016626No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG7710.4131345714867486No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC7560.40509693390918544No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGGGAGACAGGCCC7470.4002743513626475No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT7230.38741413123854634No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCTTCTGGCAGG6830.36598043103171113No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC6830.36598043103171113No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6820.36544458852654027No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA6790.36383706101102764No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG6730.3606220059800024No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG6680.3579427934541479No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG6240.3343657232266292No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC6130.32847145566974956No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGTCCCTGTGCTCGG6070.32525640063872424No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC5950.3188262905766737No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCCTGGGTGG5940.31829044807150286No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA5860.3140037080301358No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT5850.3134678655249649No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACCCCCTCTCCAC5730.30703775546291434No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC5590.299535960390522No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC5450.2920341653181297No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5360.28721158277159176No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5340.28613989776125004No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC5300.2839965277405665No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCAGGGCAGCGT5250.2813173152147121No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGTTTCCTGGGGG5210.27917394519402855No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG5130.2748872051526615No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGAGCCGCGGCTGG5020.26899293759578186No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT5020.26899293759578186No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC4930.26417035504924397No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG4860.2604194575130478No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT4840.259347772502706No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG4790.2566685599768516No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT4740.2539893474509972No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA4710.2523818199354846No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT4700.2518459774303137No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCGCCTCCTGCTCAGCC4550.2438083398527505No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGTTTCCTGGGGG4510.24166496983206695No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGTGGGGATTCAGAG4500.24112912732689606No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4430.2373782297906999No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGGAGACCCAGCGCG4400.23577070227518726No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGTGTCCTGCGTG4390.23523485977001637No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG4310.23094811972864934No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4250.22773306469762405No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGGTCTGGGTG4240.2271972221924532No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTGAGGGCTGGCT4120.22076711213040262No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCTGGTTCCCACC4060.21755205709937733No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGCACAATG4020.21540868707869382No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGACCCGGTTCCCA4010.21487284457352296No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGATGTAAAACGAC3920.21005026202698504No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTGAGAAGGACCT3890.20844273451147238No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG3870.2073710495011306No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGCAGGAAGCACCT3860.20683520699595975No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA3770.20201262444942183No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3760.20147678194425092No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCCAGCAGGTGGC3700.19826172691322566No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG3660.19611835689254214No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG3640.19504667188220037No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT3540.18968824683049157No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG3490.18700903430463717No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3230.1730771291701943No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG3210.17200544415985253No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTCCTGGCCGCCC3130.1677187041184855No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG3110.16664701910814372No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCCTCCAGCCTGG3050.16343196407711846No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCCACAGAGGGCA2960.15860938153058052No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2950.15807353902540966No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGTACCCCTGTGC2910.15593016900472612No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGACTGTTCCCACC2900.15539432649955526No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTTGACCATGAGTC2890.15485848399438437No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT2670.14306994888062502No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACTGGCATGACTT2580.1382473663340871No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTCCTGTTGTG2550.13663983881857444No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGTGGCTCCT2550.13663983881857444No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2540.13610399631340359No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTGTGTTGGCCAAC2460.13181725627203653No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACGTCATGCTGTTCTT2450.13128141376686564No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTAGCTCACT2330.12485130370481506No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGATCCT2330.12485130370481506No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTCCAGGATGGC2280.12217209117896069No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCTGTCATCTCT2170.116277823622081No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTGCACCTCTGA1960.10502513101349252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)