FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119621
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT4740.3962514943028398No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT4060.3394052883690991No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC3710.31014621178555607No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3470.29008284498541226No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3080.2574798739351786No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT2980.24912013776845202No Hit
NNGGANNNNGNTATGNNNAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA2950.24661221691843405No Hit
NNGGANNNNGNTATGNNNGTGCAGTCCTCAGGCCACACCTCCAACCCCGT2920.24410429606841608No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2910.24326832245174343No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2760.23072871820165353No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2710.2265488501182903No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2690.22487690288494497No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2640.22069703480158165No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2610.2181891139515637No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2600.21735314033489106No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA2580.2156811931015457No Hit
NNGGANNNNGNTATGNNNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA2530.21150132501818242No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA2520.21066535140150977No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2490.20815743055149183No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2470.20648548331814648No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2330.19478185268472925No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT2330.19478185268472925No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2320.19394587906805663No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGACACCCAAGACA2300.19227393183471128No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2290.19143795821803866No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2250.18809406375134802No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT2240.18725809013467534No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2220.18558614290133002No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC2200.18391419566798473No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2190.18307822205131205No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT2160.18057030120129408No Hit
NNGGANNNNGNTATGANNAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA2070.17304653865124017No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA2030.16970264418454956No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2000.16719472333453156No Hit
NNGGANNNNGNTATGANNGTGCAGTCCTCAGGCCACACCTCCAACCCCGT1990.1663587497178589No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1990.1663587497178589No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1970.1646868024845136No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1960.16385082886784094No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1950.16301485525116827No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1930.16134290801782294No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1920.1605069344011503No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1910.15967096078447765No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1860.15549109270111436No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1840.15381914546776904No Hit
NNGGANNNNGNTATGANNCTCACCAAGAGTGCCCCACGACACCCAAGACA1830.1529831718510964No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1810.15131122461775107No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1800.15047525100107842No Hit
NNGGANNNNGNTATGANNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA1800.15047525100107842No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGTCGTTTTACAAT1780.14880330376773307No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1770.14796733015106045No Hit
NNGGANNNNGNTATGANNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA1770.14796733015106045No Hit
NNGGANNNNGNTATGANNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1760.14713135653438777No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1750.1462953829177151No Hit
NAGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1740.14545940930104248No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1710.14295148845102448No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1630.13626369951764322No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1610.1345917522842979No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1590.1329198050509526No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1560.1304118842009346No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA1550.12957591058426196No Hit
NNGGANNNNGNTATGNNNGCTACGGGCAATCACTCTCCTAGTGGCATGAA1540.1287399369675893No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCGGTACAGTAACT1520.127067989734244No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1510.12623201611757134No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1490.12456006888422601No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1460.12205214803420804No Hit
NNGGANNNNGNTATGNNNCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT1430.11954422718419007No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1430.11954422718419007No Hit
NNGGANNNNGNTATGNNNGTGCAGTCCTCAGGCCACACCTCCAACCAGGA1420.11870825356751741No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1410.11787227995084476No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1410.11787227995084476No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGACACCTCCTCTGG1410.11787227995084476No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1400.1170363063341721No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1370.11452838548415413No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1320.11034851740079082No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1310.10951254378411816No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1310.10951254378411816No Hit
NNGGANNNNGNTATGNNNGCAGCCACCCTGAACACACTGGGCAGGAAGAA1300.10867657016744553No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1290.10784059655077285No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1260.10533267570075489No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1260.10533267570075489No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1250.10449670208408222No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1250.10449670208408222No Hit
NAGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1250.10449670208408222No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1240.10366072846740956No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1240.10366072846740956No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACCACCGTGATCTCTC1230.1028247548507369No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1230.1028247548507369No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1230.1028247548507369No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1220.10198878123406425No Hit
NNGGANNNNGNTATGANNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1220.10198878123406425No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCTTTTTAAATGAG1220.10198878123406425No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1210.1011528076173916No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1200.10031683400071893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)