FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183280
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG50622.7618943692710607No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA45612.488542121344391No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA37372.03895678742907No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC37322.0362287210824968No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC36672.0007638585770406No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG35071.913465735486687No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG30251.650480139676997No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA29941.633566128328241No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT29421.605194238323876No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT29261.5964644260148406No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA28871.575185508511567No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG27661.5091663029244873No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA25821.4087734613705807No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG24181.3192928852029682No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA23581.2865560890440857No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG22191.210715844609341No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG21961.1981667394151025No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC21851.1921649934526406No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA21701.1839807944129201No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC21551.1757965953731995No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG20021.092317765168049No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT19771.0786774334351812No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG16250.8866215626364033No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG15580.8500654735923178No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT15430.8418812745525972No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC14640.7987778262767351No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC14270.7785901353120908No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG13430.7327586206896551No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG13250.7229375818419903No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT13090.7142077695329551No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA12820.6994762112614579No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT12600.6874727193365343No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC12380.6754692274116106No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG12290.6705587079877783No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG12040.6569183762549106No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT11720.6394587516368397No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC11670.6367306852902662No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA11220.6121780881711043No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG10590.5778044522042776No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC9760.5325185508511566No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC9570.5221518987341772No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG9520.5194238323876037No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT9380.5117852466171977No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC9220.5030554343081624No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG9190.5014185945002182No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT9170.5003273679615888No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCCTGCCA9070.49487123526844173No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC9070.49487123526844173No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA9020.49214316892186816No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT8990.49050632911392406No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG8960.48886948930597995No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA8860.4834133566128328No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC8840.4823221300742034No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC8720.47577477084242686No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC8450.46104321257092973No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT8110.4424923614142296No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT8100.44194674814491486No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG8060.439764295067656No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC7890.430488869489306No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCCCAGCCTCT7890.430488869489306No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA7480.40811872544740285No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA7450.40648188563945875No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA7310.3988432998690528No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC7310.3988432998690528No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT7300.3982976865997381No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGATTTGGGGC7200.39284155390659103No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA6800.37101702313400264No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC6560.3579223046704496No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC6500.3546486250545613No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC6170.3366433871671759No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTTGGCCCCAATCC6150.3355521606285465No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCACGTGCAGGA6080.33173286774334354No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT5930.32354866870362287No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCTGCTGGCTGGTG5830.31809253601047577No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGGCAGGAGAG5780.31536446966390225No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC5650.308271497162811No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT5540.3022697512003492No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC5270.287538192928852No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG5210.28426451331296376No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT5190.28317328677433434No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG5150.2809908336970755No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG5110.27880838061981666No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC5090.27771715408118725No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC5030.274443474465299No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCCACGGCCATTCA5030.274443474465299No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGACCAACAATGGCCTG4950.2700785683107813No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG4730.25807507638585775No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG4670.25480139676996943No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGTGTCAGGGGGCTGCT4640.25316455696202533No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA4550.24825403753819292No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCACGGTCACTG4440.24225229157573114No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACAGCACTGCC4390.2395242252291576No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4260.23243125272806633No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT4190.2286119598428634No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCGGACTC4140.22588389349628984No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGGTCTGGGTG4050.22097337407245743No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGCAAATGCAG3840.20951549541684852No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTGGCCTGTTCG3830.20896988214753384No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC3760.20515058926233085No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGCTGGACTGTGAG3720.202968136185072No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT3720.202968136185072No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA3710.20242252291575732No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG3630.19805761676123962No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA3580.1953295504146661No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3570.1947839371453514No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC3540.19314709733740726No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3350.18278044522042777No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC3350.18278044522042777No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG3350.18278044522042777No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3350.18278044522042777No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG3290.17950676560453951No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG3290.17950676560453951No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT3270.1784155390659101No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG3230.17623308598865123No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGATTATTATGGGAT3220.17568747271933652No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG2950.16095591444783935No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2880.1571366215626364No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAAGGACAGCTGCC2880.1571366215626364No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGTTCCTGGCC2880.1571366215626364No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2870.1565910082933217No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG2840.15495416848537757No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGGCTGGAGTGC2760.1505892623308599No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCCGGTGCCCATG2750.1500436490615452No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT2730.14895242252291574No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG2680.1462243561763422No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC2640.14404190309908338No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC2530.13804015713662157No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT2520.13749454386730686No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCGGAGGGGGGTCA2500.13640331732867744No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGAGGGAGGGGTG2500.13640331732867744No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2490.13585770405936273No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGATTCAGGTGATCC2480.13531209079004802No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG2440.13312963771278916No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2370.12931034482758622No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2360.1287647315582715No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2310.12603666521169796No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGATCCTCCC2260.12330859886512441No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGGCCCAAGTCA2210.12058053251855085No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCCCTCGTTCCC2210.12058053251855085No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCTGAACCCTCGTG2110.11512439982540375No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG1910.10421213443910955No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGGC1890.10312090790048013No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG1870.10202968136185071No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGACCTAGTCCACG1850.1009384548232213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)