FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183280
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT45692.4929070274989087No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG42472.317219554779572No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT40992.2364687909209953No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT35321.9271060672195548No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT33301.8168921868179835No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA32801.789611523352248No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC29181.5920995198603232No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC28431.5511785246617198No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT27911.5228066346573548No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC27841.5189873417721518No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA27171.4824312527280663No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC24631.3438454823221302No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA22711.239087734613706No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA22701.238542121344391No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG22551.2303579223046706No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC21551.1757965953731995No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT20981.144696639022261No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC20361.110868616324749No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT19721.0759493670886076No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC18491.0088389349628983No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT17910.9771933653426452No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA17230.9400916630292449No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG17010.9280881711043213No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC16460.8980794412920122No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG16410.8953513749454387No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT15680.8555216062854649No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC13520.7376691401134876No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG13480.7354866870362288No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC13270.7240288083806198No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG12450.6792885202968136No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT12430.6781972937581842No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG12000.6547359231776516No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA11030.6018114360541249No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC10790.5887167175905719No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG10430.5690746398952422No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT10360.5652553470100393No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT10200.556525534701004No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGTGGGGACTGGA9990.5450676560453951No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG9650.5265168048886949No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG9640.5259711916193801No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG9450.5156045395024007No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC9380.5117852466171977No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT9330.5090571802706242No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT9280.5063291139240507No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC9130.49814491488432994No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA8980.4899607158446093No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT8790.47959406372762986No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG8580.468136185072021No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA8560.4670449585333915No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT8510.46431689218681793No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC8350.45558707987778263No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT8280.4517677869925797No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG8080.4408555216062855No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCATGCCTGTAG7840.42776080314273246No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG7630.4163029244871236No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT7610.4152116979484941No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA7550.4119380183326058No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC7450.40648188563945875No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT7400.4037538192928852No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG7330.39993452640768223No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACTGGAA7320.39938891313836755No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG7250.39556962025316456No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT7200.39284155390659103No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC7190.3922959406372763No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT7130.38902226102138804No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT6740.3677433435181144No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC6630.36174159755565255No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG6620.36119598428633787No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCATCCTGCACGTGG6130.33446093408991706No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC6070.3311872544740288No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT6010.32791357485814054No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCCAAGCCTGGGTG5790.31591008293321693No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC5770.3148188563945875No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCAGTTCTCACTCT5750.31372762985595815No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC5670.3093627237014404No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG5600.30554343081623747No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGGGGGAGGAGGTC5570.3039065910082933No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCCCCCAACTTCAC5450.2973592317765168No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA5270.287538192928852No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC5210.28426451331296376No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA5100.278262767350502No Hit
CAGGAAACAGCTATGACCCCCCTCCGCTGGTTGAATGGCCGTGGCTGTCT4960.27062418158009605No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA4920.26844172850283715No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT4860.2651680488869489No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCAGCAATGA4790.26134875600174595No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG4790.26134875600174595No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG4770.26025752946311653No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC4680.2553470100392842No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA4390.2395242252291576No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC4380.23897861195984285No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGTACC4360.23788738542121343No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG4330.23625054561326933No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC4140.22588389349628984No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGACCTCTCCTCCT4100.223701440419031No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC4090.22315582714971627No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGAACGGGAGGGCG4060.22151898734177217No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCTTGAGTCTGG4000.2182453077258839No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG3890.2122435617634221No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC3860.21060672195547794No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA3720.202968136185072No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAGTGTTCC3660.19969445656918378No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCCAGCACCTGTG3650.19914884329986904No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACAGGCCACCGCA3540.19314709733740726No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3510.1915102575294631No Hit
CAGGAAACAGCTATGACCCCCCTCCGCTGGTTGAATGGCAGGAAACAGCT3480.18987341772151897No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTGGGGACGCACC3470.18932780445220426No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC3420.1865997381056307No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA3340.18223483195111306No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC3340.18223483195111306No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT3320.18114360541248364No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGTGCAGGGCA3190.1740506329113924No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA3160.1724137931034483No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC3150.17186817983413358No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCACTGGCCGTCG3140.17132256656481887No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCCGGTTTCATTG3040.16586643387167177No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA3030.16532082060235706No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2900.15822784810126583No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG2710.14786119598428632No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG2710.14786119598428632No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA2710.14786119598428632No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA2690.1467699694456569No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC2640.14404190309908338No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGATAGGAGGATCAC2640.14404190309908338No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA2640.14404190309908338No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGCGCTCCAT2630.14349628982976867No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2620.14295067656045396No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2540.13858577040593628No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGAACGAGGGCCAGT2430.13258402444347445No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCACCCCTCCCTCT2400.13094718463553034No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT2360.1287647315582715No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGCCAGGGT2340.1276735050196421No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2320.12658227848101267No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC2240.12221737232649499No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA2240.12221737232649499No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG2190.11948930597992143No Hit
CAGGAAACAGCTATGACCCCCCTCCGCTGGTTGAATGGCTCCACGAGGGT2130.11621562636403317No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCCGGGTT2090.11403317328677433No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCGCCATTCA2080.11348756001745963No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTGGGTGTCTGC1950.10639458751636839No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGCCGT1900.10366652116979484No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC1870.10202968136185071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)