FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54978
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2010.36560078576885296No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1840.3346793262759649No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1710.3110335043108153No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1670.30375786678307687No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1670.30375786678307687No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1420.25828513223471206No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1370.2491905853250391No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1350.24555276656116992No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1330.24191494779730072No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1230.2237258539779548No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1210.22008803521408565No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1210.22008803521408565No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1200.21826912583215105No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1190.21645021645021645No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1190.21645021645021645No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1160.2109934883044127No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1140.2073556695405435No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1140.2073556695405435No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1140.2073556695405435No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1100.20008003201280514No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1090.19826112263087053No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1070.19462330386700136No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1050.19098548510313218No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1040.18916657572119758No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1020.1855287569573284No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA990.18007202881152462No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC990.18007202881152462No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA970.17643421004765542No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC960.17461530066572084No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG960.17461530066572084No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC920.16733966313798246No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA920.16733966313798246No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC920.16733966313798246No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG900.16370184437411328No Hit
NNGGANNNNGNTATGNNNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT890.16188293499217868No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC880.1600640256102441No Hit
NAGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA860.1564262068463749No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG850.15460729746444032No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT840.15278838808250572No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC810.14733165993670194No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC810.14733165993670194No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA800.14551275055476737No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT800.14551275055476737No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT800.14551275055476737No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC790.14369384117283276No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG770.1400560224089636No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG760.13823711302702898No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC760.13823711302702898No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG760.13823711302702898No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA750.1364182036450944No Hit
NNGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT730.13278038488122523No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA720.13096147549929063No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC720.13096147549929063No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG710.12914256611735603No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC690.12550474735348685No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT690.12550474735348685No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA680.12368583797155226No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC670.12186692858961766No Hit
NNGGANNNNGNTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT670.12186692858961766No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC670.12186692858961766No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC660.12004801920768307No Hit
NNGGANNNNGNTATGANNACCGTTAAGCCACACCAAATGGTGAGCCCTGC660.12004801920768307No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC660.12004801920768307No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA660.12004801920768307No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT650.11822910982574848No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT650.11822910982574848No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT640.11641020044381388No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG630.1145912910618793No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT610.1109534722980101No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG610.1109534722980101No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG600.10913456291607553No Hit
NNGGANNNNGNTATGNNNACCCCACCTGCTCAAAAGACACACTCCTAAAA600.10913456291607553No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA590.10731565353414092No Hit
NAGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC590.10731565353414092No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT590.10731565353414092No Hit
NNGGANNNNGNTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA590.10731565353414092No Hit
NAGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT580.10549674415220635No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT570.10367783477027175No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC570.10367783477027175No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT570.10367783477027175No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC560.10185892538833714No Hit
NNGGANNNNGNTATGANNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC560.10185892538833714No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC550.10004001600640257No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA550.10004001600640257No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC550.10004001600640257No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC550.10004001600640257No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC550.10004001600640257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)