FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63013
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC15422.4471140875692314No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC9611.5250821259105265No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG9321.4790598765334138No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA8931.4171678859917796No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT8681.3774935330804756No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG7641.2124482249694508No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT7071.1219907003316776No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC6891.0934251662355388No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG6791.077555425071017No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT6781.0759684509545648No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT5640.8950534016790187No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG5410.8585529970006189No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG5360.8506181264183581No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT5290.839509307603193No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG5200.8252265405551235No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC5090.8077698252741498No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG5090.8077698252741498No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC4940.7839652135273674No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC4850.7696824464792978No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT4770.7569866535476807No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG4630.7347690159173503No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA4610.731595067684446No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC4460.7077904559376637No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC4360.691920714773142No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG4350.6903337406566898No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA4350.6903337406566898No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA4200.6665291289099075No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG4120.6538333359782902No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC4080.6474854395124815No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT4020.6379635948137686No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG3940.6252678018821514No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT3810.6046371383682733No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGCAGCTGCCATGT3810.6046371383682733No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG3770.5982892419024646No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC3750.5951152936695603No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA3660.5808325266214909No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG3320.5268754066621173No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC3270.5189405360798566No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG3230.5125926396140479No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT3170.5030707949153349No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT3130.4967228984495263No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC3120.4951359243330742No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG3000.47609223493564823No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2990.4745052608191961No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG2970.47133131258629174No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC2820.44752670083950935No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG2800.444352752606605No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG2780.4411788043737007No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2640.4189611667433704No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT2480.3935695808801359No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC2450.3888086585307794No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCAGGGCAGAAGGG2440.3872216844143272No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG2430.38563471029787505No Hit
TGTAAAACGACGGCCAGTACCAGCAATGCATTTTGTGGACCACCCATGCT2410.3824607620649707No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT2410.3824607620649707No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT2370.37611286559916207No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG2340.3713519432498056No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC2320.3681779950169013No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC2210.3507212797359275No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA2160.3427864091536667No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT2160.3427864091536667No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCAGCACCCGGCAGC2140.3396124609207624No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA2100.3332645644549537No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGCCCAGTGTGTT2070.32850364210559724No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC2070.32850364210559724No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG2050.32532969387269295No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA2000.31739482329043217No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA1980.3142208750575278No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC1970.3126339009410757No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1960.3110469268246235No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG1950.30945995270817134No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGTGTGGCCTGAG1950.30945995270817134No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT1920.30469903035881485No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA1910.3031120562423627No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA1870.29676415977655407No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT1870.29676415977655407No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT1830.29041626331074544No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA1810.2872423150778411No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG1800.2856553409613889No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAAGCAAC1750.2777204703791281No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA1700.26978559979686734No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1670.26502467744751085No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC1670.26502467744751085No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGGGAGAGT1640.26026375509815436No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1520.24122006570072843No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT1470.2332851951184676No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT1450.23011124688556328No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC1420.22535032453620685No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1400.2221763763033025No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT1400.2221763763033025No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG1380.2190024280703982No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1380.2190024280703982No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG1340.21265453160458953No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1310.20789360925523304No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACTCTCTGCCG1300.20630663513878086No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAGCCTCC1270.20154571278942443No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC1260.19995873867297226No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG1240.19678479044006794No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA1230.19519781632361577No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG1220.1936108422071636No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC1160.18408899750845065No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG1160.18408899750845065No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1160.18408899750845065No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1150.18250202339199847No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACTCTCTACCG1150.18250202339199847No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1150.18250202339199847No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1140.18091504927554633No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT1130.17932807515909416No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1100.17456715280973767No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG1100.17456715280973767No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA1090.1729801786932855No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG1090.1729801786932855No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCACTTCCTGC1060.16821925634392904No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGGCAGAAGGG1050.16663228222747686No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC1050.16663228222747686No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1050.16663228222747686No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT1040.16504530811102472No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGGTGGAGTGTGT1030.16345833399457255No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG1020.16187135987812037No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC1020.16187135987812037No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCAATAATGCTGCT1000.15869741164521609No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA1000.15869741164521609No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG930.14758859283005096No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATAGCTGTTTCCTG920.1460016187135988No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCACATTGGAGT870.13806674813133796No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA870.13806674813133796No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG860.13647977401488584No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC850.13489279989843367No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCAGTCACCCCTGCA850.13489279989843367No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGAG850.13489279989843367No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGGCTGAAAAGAT830.13171885166552932No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTACGGCACTGGT800.12695792931617284No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCGGCAGCGACAT790.1253709551997207No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGGGGCGAGTGT780.12378398108326855No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA760.12061003285036422No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTGCTGCCTCT750.11902305873391206No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCGGCCCCTG740.11743608461745988No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC730.11584911050100773No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG730.11584911050100773No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA720.11426213638455555No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG720.11426213638455555No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT710.11267516226810342No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTGCTCACGCAA700.11108818815165125No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTCCCCCTGCTG690.1095012140351991No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG680.10791423991874692No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGTGCCATGTTTCC680.10791423991874692No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC680.10791423991874692No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCTGAATGCGTGTT680.10791423991874692No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCATCCTCCTCTTC670.10632726580229476No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG670.10632726580229476No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC660.1047402916858426No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCAGCACGACG650.10315331756939043No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA640.10156634345293827No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTACATCCTGGAGA640.10156634345293827No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC640.10156634345293827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)