FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134377
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA6720.5000855801215982No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA4760.3542272859194654No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC4360.3244602871027036No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4040.3006466880492941No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT3960.29469328828594177No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT3910.29097241343384656No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG3600.26790298935085616No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3480.25897288970582766No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT3460.25748453976498953No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG3270.24334521532702771No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3190.23739181556367536No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC3110.23143841580032298No Hit
NNGGANNNNGNTATGNNNCCCATCCACTAGTCGGGGAGTGTCATTGGTGC3070.22846171591864678No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2850.2120898665694278No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2840.21134569159900876No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2840.21134569159900876No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG2810.20911316668775162No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT2760.2053922918356564No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2730.20315976692439927No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT2720.2024155919539802No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT2670.198694717101885No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2660.19795054213146596No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2650.19720636716104692No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2580.19199714236811358No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT2570.19125296739769454No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2460.18306704272308505No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2440.18157869278224698No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2420.18009034284140887No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2380.1771136429597327No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2360.1756252930188946No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2330.17339276810763746No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT2290.17041606822596128No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2190.16297431852177086No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2170.16148596858093275No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC2170.16148596858093275No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2160.16074179361051372No Hit
NNGGANNNNGNTATGANNACCTGGCAGCCCCAAATCTGCGGGTGACCACG2070.1540442188767423No Hit
NNGGANNNNGNTATGANNCCCATCCACTAGTCGGGGAGTGTCATTGGTGC2070.1540442188767423No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2060.15330004390632324No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2040.15181169396548516No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCAGGCAC2020.15032334402464706No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2010.14957916905422802No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1990.14809081911338995No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1960.1458582942021328No Hit
NNGGANNNNGNTATGANNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT1940.1443699442612947No Hit
NNGGANNNNGNTATGNNNGCAGCCACCCTGAACACACTCCACCAGGAAGA1920.14288159432045663No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1870.1391607194683614No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1860.13841654449794236No Hit
NNGGANNNNGNTATGNNNGCAGTGACAGGGCAAGGAGGGTGGATGGATCC1820.13543984461626618No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1810.13469566964584712No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1800.13395149467542808No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1800.13395149467542808No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1800.13395149467542808No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1780.13246314473459No Hit
NAGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1780.13246314473459No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1760.1309747947937519No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1750.13023061982333287No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1720.12799809491207573No Hit
NAGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1710.1272539199416567No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1690.1257655700008186No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1670.1242772200599805No Hit
NAGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1670.1242772200599805No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1640.12204469514872336No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1630.12130052017830432No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1610.11981217023746622No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1590.11832382029662815No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1570.11683547035579005No Hit
NAGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1570.11683547035579005No Hit
NAGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT1570.11683547035579005No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1560.11609129538537101No Hit
NNGGANNNNGNTATGNNNCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC1560.11609129538537101No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC1540.11460294544453292No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1520.11311459550369482No Hit
NNGGANNNNGNTATGNNNGTGCAGTCCTCAGGCCACACCTCCAACCCCGT1510.11237042053327578No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1480.11013789562201864No Hit
NAGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1480.11013789562201864No Hit
NAGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1470.10939372065159961No Hit
NAGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1470.10939372065159961No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1470.10939372065159961No Hit
NNGGANNNNGNTATGNNNTCCCACTTGCTCTTTGAAGGCAGGCACTACGT1470.10939372065159961No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCAGGCAC1450.1079053707107615No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1450.1079053707107615No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1440.10716119574034247No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGCCCAAGACACTG1430.10641702076992343No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1410.10492867082908533No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1400.10418449585866628No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAAGGGGACATGGCC1390.10344032088824724No Hit
NAGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1390.10344032088824724No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1390.10344032088824724No Hit
NNGGANNNNGNTATGNNNCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT1380.1026961459178282No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1380.1026961459178282No Hit
NAGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1370.10195197094740917No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTTCCTGCAAAACA1370.10195197094740917No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT1360.1012077959769901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)