FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130059
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT37322.8694669342375385No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT33172.5503809809394196No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC27822.1390292098201584No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA25331.9475776378412875No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT24251.864538401802259No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA20241.5562167939166072No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT20001.5377636303523785No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC19901.5300748122006167No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT19441.4947062487025118No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT19331.4862485487355739No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC18901.4531866306829977No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC18331.4093603672179549No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA17421.3393921220369218No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC15711.2079133316417934No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG14791.137176204645584No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC12910.9926264233924604No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT12510.9618711507854127No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC12190.9372669326997747No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT11690.8988228419409652No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT10820.8319301240206368No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT10710.8234724240536987No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT10620.816552487717113No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT10560.8119391968260559No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC10430.8019437332287654No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG10210.7850283332948892No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG9970.7665751697306606No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG9940.7642685242851321No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9690.7450464789057274No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC9670.7435087152753751No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA9430.7250555517111464No Hit
CAGGAAACAGCTATGACCAGCAACATACCGTTTGTAAACAACTGGCCGTC9200.7073712699620941No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA9040.6950691609192751No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG8810.6773848791702227No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC8780.6750782337246941No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC8730.6712338246488132No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC8340.6412474338569418No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC7970.6127988066954229No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA7960.6120299248802467No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG7400.56897254323038No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6900.5305284524715707No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTAGGCCA6560.5043864707555802No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT6480.49823541623417067No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA6340.487471070821704No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT6280.4828577799306469No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC6110.46978678907265164No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA6110.46978678907265164No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG5920.4551780345843041No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTAGGAGGCTGTC5910.45440915276912786No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC5900.45364027095395165No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC5820.44748921643254214No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5750.4421070437263089No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATCAGGGTGG5720.4398003982807802No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC5710.4390315164656041No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC5660.43518710738972316No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC5590.42980493468348974No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT5540.4259605256076089No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5390.414427298379966No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG5360.41212065293443745No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG5340.4105828893040851No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCGCTCAGCTCC5320.4090451256737327No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC5270.40520071659785173No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG5190.3990496620764422No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT5120.3936674893702089No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC4970.3821342621425661No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4970.3821342621425661No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA4910.3775209712515089No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCGTCGTTTTC4910.3775209712515089No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC4870.37444544399080415No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT4840.3721387985452756No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG4810.36983215309974704No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG4710.3621433349479852No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC4650.357530044056928No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTGCCTCCCGAC4530.34830346227481374No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT4340.33369470778646615No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC4210.32369924418917567No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC4210.32369924418917567No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGCCCCACGTTCCA4180.3213925987436471No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC4150.31908595329811856No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG4050.3113971351463567No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGATGCTGCCTCCT3950.3037083169945948No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCCCGAAGCGTTGG3940.30293943517941857No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA3760.2890995625062472No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTACACCTCCTTC3730.2867929170607186No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG3650.28064186253930906No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG3590.27602857164825195No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGGGAGATCGG3540.272184162572371No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG3490.2683397534964901No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC3470.2668019898661377No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC3450.2652642262357853No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3390.26065093534472816No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3280.2521932353777901No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT3260.2506554717474377No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG3150.24219777178049962No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC3050.23450895362873772No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3010.23143342636803296No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCATTCTCAGGACT3010.23143342636803296No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGAGTGCAGC2990.22989566273768058No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC2970.2283578991073282No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2820.21682467187968535No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC2780.21374914461898062No Hit
CAGGAAACAGCTATGACCAACACCCTTGGCCTCCCTCACTGGCCGTCGTT2760.21221138098862824No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT2750.21144249917345206No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2750.21144249917345206No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT2740.21067361735827586No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2660.20452256283686635No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGTCATTGGTG2660.20452256283686635No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGGTCAACCTGCT2600.1999092719458092No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG2570.19760262650028063No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGACGTGTCCGCT2530.1945270992395759No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG2520.1937582174243997No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2510.19298933560922352No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2490.19145157197887114No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCTCATAGGCAG2470.18991380834851876No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2420.1860693992726378No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2370.18222499019675686No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2330.1791494629360521No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAAATTCCAGCT2290.17607393567534735No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT2230.1714606447842902No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT2220.17069176296911404No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGTTGCCCTGC2200.16915399933876166No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCAGGAAACAGCT2160.1660784720780569No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2140.16454070844770452No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC2120.1630029448173521No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA2110.16223406300217594No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG2070.15915853574147118No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCAGCAGCTATGA2050.1576207721111188No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACACAGACACACA2040.1568518902959426No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT2020.15531412666559022No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGTGCGGA2010.15454524485041404No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC2000.15377636303523784No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA1980.1522385994048855No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC1960.1507008357745331No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT1930.14839419032900453No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT1910.14685642669865215No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1900.14608754488347597No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG1880.1445497812531236No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGCTGCCCTGC1870.1437808994379474No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1870.1437808994379474No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC1830.14070537217724263No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1760.1353231994710093No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCTGAGCCATGGG1760.1353231994710093No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAAGAGGGGGCAG1710.13147879039512836No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCTTGGCGT1700.13070990857995218No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1670.1284032631344236No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGAGCTGCGAGCAT1650.12686549950407122No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAAGGCACTACG1650.12686549950407122No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTGCCTATAAT1640.12609661768889505No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA1630.12532773587371884No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG1620.12455885405854267No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1610.12378997224336648No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG1610.12378997224336648No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG1600.12302109042819029No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1570.12071444498266172No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1570.12071444498266172No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCACATCCCAGGG1560.11994556316748553No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG1560.11994556316748553No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1540.11840779953713315No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAATAAATAGGCAGC1540.11840779953713315No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1520.11687003590678077No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGGAGAGGAGTC1510.11610115409160458No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGCTTGTCATT1500.1153322722764284No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1470.11302562683089981No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA1460.11225674501572362No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC1450.11148786320054743No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC1440.11071898138537124No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCAGGAAACAGCA1430.10995009957019505No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA1420.10918121775501886No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA1400.1076434541246665No Hit
CAGGAAACAGCTATGACCAACTTTTAGGTAGAGCCAAAGAACTGGCCGTC1310.1007235177880808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)