FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39953
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1480.3704352614321828No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1430.3579205566540685No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1250.3128676194528571No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1210.3028558556303657No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1160.2903411508522514No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1100.2753235051185143No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1050.26280880034039994No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1010.2527970365179085No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1010.2527970365179085No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT990.24779115460666282No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC980.24528821365103998No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC920.23027056791730285No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC910.22776762696167996No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT880.22025880409481138No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC880.22025880409481138No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT870.21775586313918854No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT840.21024704027231997No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC830.20774409931669713No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT800.20023527644982855No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT780.19522939453858285No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT770.19272645358295998No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC760.19022351262733714No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG750.18772057167171427No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC740.1852176307160914No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT740.1852176307160914No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT720.1802117488048457No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC710.17770880784922283No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC710.17770880784922283No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT660.16519410307110854No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC660.16519410307110854No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT650.1626911621154857No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT650.1626911621154857No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC630.15768528020424No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC630.15768528020424No Hit
NAGGAANNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC630.15768528020424No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC620.15518233924861713No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT610.1526793982929943No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC610.1526793982929943No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA610.1526793982929943No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT610.1526793982929943No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT600.1501764573373714No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC590.14767351638174855No Hit
NAGGAANNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT580.1451705754261257No Hit
NAGGAANNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT580.1451705754261257No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT580.1451705754261257No Hit
NAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT570.14266763447050285No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA570.14266763447050285No Hit
NAGGAANNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT560.14016469351487998No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC560.14016469351487998No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT560.14016469351487998No Hit
NNGGAAANNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC550.13766175255925714No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT550.13766175255925714No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA540.13515881160363427No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT540.13515881160363427No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC540.13515881160363427No Hit
NAGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC530.1326558706480114No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT530.1326558706480114No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT530.1326558706480114No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT520.13015292969238854No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG520.13015292969238854No Hit
NNGGAAANNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT520.13015292969238854No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT520.13015292969238854No Hit
NNGGAAANNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT520.13015292969238854No Hit
NNGGAAANNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC510.1276499887367657No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA510.1276499887367657No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC500.12514704778114283No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT500.12514704778114283No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT500.12514704778114283No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC500.12514704778114283No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA490.12264410682551999No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC490.12264410682551999No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC480.12014116586989713No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC480.12014116586989713No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT480.12014116586989713No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA480.12014116586989713No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT480.12014116586989713No Hit
NAGGAANNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC480.12014116586989713No Hit
NAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC480.12014116586989713No Hit
NAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC480.12014116586989713No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC470.11763822491427428No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT470.11763822491427428No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC460.11513528395865143No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT460.11513528395865143No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT460.11513528395865143No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA460.11513528395865143No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT450.11263234300302855No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT450.11263234300302855No Hit
NAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC440.11012940204740569No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT440.11012940204740569No Hit
NNGGAAANNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC440.11012940204740569No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG430.10762646109178284No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG430.10762646109178284No Hit
NAGGAAANAGCTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT430.10762646109178284No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA430.10762646109178284No Hit
NAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC420.10512352013615998No Hit
NAGGAAANAGCTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC420.10512352013615998No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG420.10512352013615998No Hit
NNGGAAANNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT410.10262057918053713No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA410.10262057918053713No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC410.10262057918053713No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC410.10262057918053713No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT410.10262057918053713No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA410.10262057918053713No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT410.10262057918053713No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT410.10262057918053713No Hit
NAGGAANNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC400.10011763822491428No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA400.10011763822491428No Hit
NAGGAAANAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT400.10011763822491428No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT400.10011763822491428No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT400.10011763822491428No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT400.10011763822491428No Hit
NAGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC400.10011763822491428No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC400.10011763822491428No Hit
NNGGAAANNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC400.10011763822491428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)