FastQCFastQC Report
Wed 8 May 2024
EGAF00003246569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100434
Total Bases30.1 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC34463.4311089869964353No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA24662.4553438078738274No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG18041.796204472588964No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC16761.668757592050501No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT16651.6578051257542266No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC15611.5542545353167254No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC15181.5114403488858354No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA15121.505466276360595No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA14981.4915267738017006No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA14531.446721229862397No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT14421.435768763566123No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC13121.3063305255192466No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC12761.2704860903678037No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG11441.1390564948125137No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC11211.1161558834657586No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT10751.0703546607722485No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA10681.0633849094928012No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC10411.036501583129219No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG10221.0175836867992911No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT10191.0145966505366708No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT9560.951868889021646No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT9450.9409164227253719No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT9360.9319553139375112No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9200.9160244538702033No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG9090.9050719875739293No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG9050.9010892725571021No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT8960.8921281637692414No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA8690.8652448374056595No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT8490.8453312623215246No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA7770.773642392018639No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT7690.7656769619849851No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTAGCTAG7580.754724495688711No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT7530.7497461019176772No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA7430.7397893143756098No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7390.7358065993587829No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC7330.7298325268335425No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT7320.7288368480793357No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG7310.7278411693251289No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7270.723858454308302No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC7230.7198757392914751No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT7220.7188800605372683No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC7100.7069319154867874No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA6880.685026982894239No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG6560.6531652627596233No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG6540.6511739052512098No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG6510.6481868689885895No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG6490.6461955114801761No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT6470.6442041539717626No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC6240.6213035426250074No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5760.5735109624230839No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG5750.5725152836688772No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT5600.5575801023557759No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5570.5545930660931557No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA5480.545631957305295No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5370.5346794910090208No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5340.5316924547464006No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC5270.5247227034669534No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG5140.5117788796622658No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5100.5077961646454388No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5080.5058048071370254No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA5040.5018220921201983No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC5010.4988350558575781No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG4880.4858912320528905No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT4790.47693012326502976No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT4690.4669733357229623No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4670.46498197821454884No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA4290.42714618555469264No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG4130.4112153254873847No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA3880.3863233566322162No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA3620.3604357090228409No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3600.3584443515144274No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG3600.3584443515144274No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3470.3455005277097397No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT3260.32459127387139813No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC3150.31363880757512397No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG3070.30567337754147No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGGCTGAAAAGAT2840.2827727661947149No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG2840.2827727661947149No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT2740.2728159786526475No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA2690.2678375848816138No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG2690.2678375848816138No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG2670.2658462273732003No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA2630.26186351235637334No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC2560.25489376107692613No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG2510.24991536730589242No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG2460.2449369735348587No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA2400.23896290100961826No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG2360.2349801859927913No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT2330.23199314973017104No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2320.23099747097596432No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2260.22502339845072386No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG2240.22303204094231036No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC2160.21506661090865642No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT2120.21108389589182944No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2000.19913575084134855No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG1890.18818328454507438No Hit
TGTAAAACGACGGCCAGTTCTTTGGAATTTAGTGTCTGAGCCTTGGATAC1820.18121353326562717No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1770.17623513949459346No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1730.1722524244777665No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG1730.1722524244777665No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1700.16926538821514628No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTGTGCAGGCG1680.16727403070673277No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG1640.16329131568990582No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGGAACAACT1570.1563215644104586No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1520.1513431706394249No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC1510.15034749188521815No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGTTGGGGTCCC1480.14736045562259792No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1420.14138638309735746No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA1390.13839934683473723No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTTAGCTA1390.13839934683473723No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGGGTGCTTTCTCT1380.1374036680805305No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT1380.1374036680805305No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT1350.13441663181791028No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC1220.12147280801322262No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG1220.12147280801322262No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTCCTCTATGGGCT1160.11549873548798216No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACACCTTC1120.11151602047115518No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC1120.11151602047115518No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC1100.1095246629627417No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG1090.10852898420853495No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT1060.10554194794591472No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1050.104546269191708No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGAGGTGGCCTTG1040.10355059043750126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)