FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267320
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG164986.17162950770612No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC124914.672676941493341No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG124324.650606015262607No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG75362.819093221607063No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG72982.7300613496932513No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA71162.6619781535238665No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG66122.4734400718240313No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG63962.392638036809816No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC51351.9209187490647914No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG50971.9067035762382163No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC50881.9033368247792906No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT39651.483241059404459No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC39141.4641628011372139No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA34571.2932066437228789No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC33321.2464462067933562No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG32711.2236271135717494No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC31011.1600329193475982No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG28541.0676342959748615No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG26290.9834655095017207No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG23690.8862038006883135No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC23470.8779739637887177No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG23060.8626365404758342No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG22130.8278467754002693No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG22010.8233577734550351No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA19620.7339518180457878No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC18020.6740984587759988No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAATCCTTGGAGGC17870.6684872063444561No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA17830.6669908723627114No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG17140.6411791111776148No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC16960.6344456082597636No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA16910.6325751907825827No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC15300.5723477480173574No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA15170.5674846625766872No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTTCCACAGGGACAC14980.5603770761633996No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA14790.5532694897501123No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT14640.5476582373185694No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG13100.49004937902139756No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA13030.48743079455334426No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA11500.43019601975160854No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC11300.4227143498428849No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG10990.4111177614843633No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG10940.4092473440071824No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA10800.40401017507107584No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT10760.4025138410893312No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG10040.37557982941792606No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA9680.3621128235822235No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT9440.3531348196917552No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC8360.3127338021846476No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT7060.264102947777944No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACCAGTGGCAGGA6940.25961394583270986No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA6780.253628609905731No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGCCCGGATGTTGG5840.21846476133472992No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT5370.20088283704922938No Hit
TGTAAAACGACGGCTAGTTGGAACCATTTTTGATAACTTTCTGATCACAG5160.19302708364506957No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG5150.1926530001496334No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA4810.17993416130480325No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATAGCTGTTTCCGG4810.17993416130480325No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGACAACTTTCTGATCACAG4800.17956007780936706No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG4610.1724524913960796No Hit
TGTAAAACGACGGCTAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC4350.1627263205147389No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATACCTTTCTGATCACAG4250.15898548556037706No Hit
TGTAAAACGACGGCTAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG4220.15786323507406855No Hit
TGNAAAACGACGGCCAGTTGGAACCATTTTTGATAACCTTCTGATCACAG4140.1548705671105791No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3980.14888523118360017No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA3650.1365404758342062No Hit
TGNAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGCGAGACGGGCACC3480.13018105641179112No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAACCCCGGTCGGGTTCTGG3360.12569205446655693No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACCGTGAGACGGGCACC3230.12082896902588659No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCAGGCGCCTG2900.1084842136764926No Hit
TGNAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2860.10698787969474788No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2780.10399521173125842No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG2710.10137662726320514No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2680.10025437677689661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)