FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150041
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG46893.1251457934831146No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG44692.978519204750701No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT36822.4539959077852056No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA31042.0687678701155017No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG30862.0567711492192133No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA29451.9627968355316212No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT29231.94813417665838No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG28791.9188088589118975No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT27511.8334988436494024No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA25841.7221959331116161No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC23591.572236921908012No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT22931.528248945288288No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG22301.4862604221512787No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG21411.4269433021640752No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT20701.37962290307316No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC20361.3569624302690597No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC20251.349631100832439No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA20061.3369678954419124No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA19991.3323025039822447No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC19231.2816496824201384No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG16131.0750394892062838No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC15231.0150558847248419No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC14900.9930618964149799No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT14770.9843975979898828No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT14650.9763997840590238No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13340.8890903153138141No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT12650.8431028852113757No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG12260.8171099899360842No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA11210.7471291180410687No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC10860.7238021607427304No Hit
TGTAAAACGACGGCCAGTCCAGGCATCTCCCAGCTGCGGTCATAGCTGTT10650.7098059863637273No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG10550.7031411414213449No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC10490.6991422344559154No Hit
TGTAAAACGACGGCCAGTTGTGGACCACAGTTTGCAAGTGTAAAACGACG10430.6951433274904859No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT10350.68981145153658No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTAACTCATAGG9660.6438240214341413No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG9300.6198305796415646No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC9030.6018354982971321No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG8840.5891722929066056No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA8240.5491832232523111No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT7650.5098606380922548No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGTGGGAGACCCAGC7540.5025293086556342No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG7380.49186555674782223No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC7260.4838677428169634No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC7230.48186828933424863No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT7120.474536959897628No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC7040.46920508394372207No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGACCTAGTCCACG6830.45520890956471893No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG6700.44654461113962185No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC6640.4425457041741924No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6440.42921601428942757No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT6370.4245506228297598No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG6360.4238841383355217No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6300.41988523137009215No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC6270.41788577788737746No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA6210.41388687092194804No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC6200.4132203864277098No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC5970.3978912430602302No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG5940.3958917895775155No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGGAGACCCAGCGCG5880.39189288261208605No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG5660.3772302237388447No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT5410.3605681113828887No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC5370.3579021734059357No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5060.3372411540845502No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT5050.33657466959031196No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA5000.33324224711912076No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA4980.3319092781306443No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC4950.32990982464792956No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA4940.32924334015369133No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTGTGGAGATGG4850.32324497970554716No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC4730.31524716577468825No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGTCACCCAGGC4640.3092488053265441No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG4630.3085823208323058No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA4530.3019174758899234No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAA4470.297918568924494No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4450.29658559993601746No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGCAGCATCGCCAC4420.2945861464533028No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG4380.2919202084763498No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC4370.2912537239821115No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA4230.28192294106277616No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC4210.2805899720742997No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG4160.2772575496031085No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCACAGGTGGACA4140.275924580614632No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC4120.27459161162615553No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG4090.2725921581434408No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA4080.27192567364920256No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG4080.27192567364920256No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC4040.2692597356722496No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG3960.26392785971834365No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA3950.2632613752241054No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCATCTGCCGCCC3890.25926246825867594No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA3850.256596530281723No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCAGTGCCTTGCCAG3810.25393059230477005No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA3700.24659926286814937No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC3650.24326684039695814No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTTGTAAAACGACG3550.23660199545457575No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACAAGGACTGGCT3500.23326957298338452No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC3490.2326030884891463No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC3410.22727121253524038No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACATCTCCTGA3310.22060636759285795No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAGGAATTCAG3310.22060636759285795No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3280.21860691411014324No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG3260.21727394512166673No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3240.21594097613319027No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTAGCTCACT3150.20994261568504607No Hit
TGTAAAACGACGGCCAGTTGTTTACAAACGGTATGTTGCTGGTCATAGCT3070.20461073973114013No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG3030.20194480175418716No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC2970.19794589478875774No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG2840.1892815963636606No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCCCCCAACTCCA2680.17861784445584872No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG2600.1732859685019428No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGACCGTGTCCGA2590.17261948400770455No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGCGTGTGTTCGTGC2540.16928706153651335No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT2540.16928706153651335No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATGCAGTTTTAAG2530.1686205770422751No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2520.16795409254803687No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2490.16595463906532215No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCTTCATTTCCACGTA2490.16595463906532215No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA2490.16595463906532215No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2450.16328870108836918No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTGACAGGCGGAC2400.15995627861717798No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC2390.15928979412293973No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT2330.1552908871575103No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2330.1552908871575103No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCATGACTCAAT2330.1552908871575103No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCACTGCCTAT2300.15329143367479556No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCACTCCCGCCTGG2290.15262494918055733No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGTGCTCATCAT2240.1492925267093661No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA2080.13862877480155425No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCACTCCAGCCTGG2050.1366293213188395No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCTGTGCTCCAGCC2030.13529635233036302No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCCCACT2020.1346298678361248No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1990.13263041435341008No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGTGTGTGTTCGTGC1980.13196392985917182No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTCATCCTTGGGT1960.13063096087069534No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1920.12796502289374237No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCAGACCCAAACT1890.12596556941102766No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1890.12596556941102766No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGCCCCAAGGAT1860.12396611592831291No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGTGGGGATTCAGAG1850.12329963143407469No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTTTCCTGTTCTG1810.12063369345712172No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC1770.11796775548016875No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGGAGGCCTGC1730.11530181750321579No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGATCCT1730.11530181750321579No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGCAGAGAATTC1720.11463533300897755No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCCGCAA1690.11263587952626282No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT1610.10730400357235689No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCCTCCAGCCTGG1580.10530455008964217No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCTTCTGGCAGG1580.10530455008964217No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTCCTGGCCGCCC1570.10463806559540394No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCTGTCAGG1520.10130564312421271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)