FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150041
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT42232.814564019168094No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT40682.711258922561167No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT38482.5646323338287536No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT38452.562632880346039No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC34762.3167001019721276No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT32972.1973993775034826No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC31112.073433261575169No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT27271.8175032157876847No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT27001.799508134443252No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT26531.768183363214055No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA26411.760185549283196No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT25341.688871708399704No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA21441.4289427556467897No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC20031.3349684419591978No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA20021.3343019574649597No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC19241.2823161669143768No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT18461.2303303763637938No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA18421.227664438386841No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG16891.12569231076839No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC14630.9750668150705475No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG13920.9277464159796323No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT13770.9177491485660586No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA13560.9037529741870556No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC13300.8864243773368613No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC12890.8590985130730934No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA12050.803113815557081No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG11690.7791203737645044No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT10970.7311334901793509No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10350.68981145153658No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA10310.687145513559627No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA10000.6664844942382415No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA10000.6664844942382415No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT9950.6631520717670503No Hit
CAGGAAACAGCTATGACCAGCAACATACCGTTTGTAAACAACTGGCCGTC9690.6458234749168561No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC9470.6311608160436147No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA9150.609833312227991No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9100.6065008897567998No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC7580.5051952466325871No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC7550.5031957931498724No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC7370.491199072253584No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC7230.48186828933424863No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT7150.4765364133803427No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG6970.4645396924840544No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT6910.4605407855186249No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG6690.44587812664538357No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC6530.4352143747375717No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT6390.4258835918182363No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG6380.4252171073239981No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT6180.4118874174392333No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5990.3992242120487067No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT5840.3892269446351331No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG5810.38722749115241833No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5660.3772302237388447No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC5660.3772302237388447No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT5560.3705653787964623No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA5440.3625675648656034No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG5280.3519038129577915No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC5250.3499043594750768No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT5250.3499043594750768No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG5070.33790763857878847No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA4930.3285768556594531No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC4920.3279103711652148No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA4880.3252444331882619No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT4870.3245779486940236No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGACTATGCTGAA4870.3245779486940236No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT4670.31124825880925877No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG4600.3065828673495911No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG4550.3032504448783999No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT4520.3012509913956852No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGATGCTGCCTCCT4520.3012509913956852No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA4510.30058450690144695No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA4490.29925153791297043No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCCTGGGTGACAG4480.2985850534187322No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA4470.297918568924494No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA4390.29258669297058804No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG4210.2805899720742997No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC4190.2792570030858232No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT4120.27459161162615553No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGCCTCATGTCC4110.27392512713191725No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCACTGGCCGTCG4090.2725921581434408No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT4040.2692597356722496No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA3900.2599289527529142No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCACTGGCAA3820.2545970767990083No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT3800.25326410781053177No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3790.25259762331629354No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3770.2512646543278171No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC3770.2512646543278171No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC3760.2505981698335788No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3760.2505981698335788No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGCAGATGGAGA3670.24459980938543466No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3550.23660199545457575No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTTGATGGCCTCAG3540.2359355109603375No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA3340.22260582107557267No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAAGGCACTACG3290.2192733986043815No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC3270.21794042961590498No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG3270.21794042961590498No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG3030.20194480175418716No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC3020.20127831725994896No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2950.19661292580028128No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2870.1912810498463753No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCATGGGCAACAG2850.18994808085789883No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT2780.18528268939823114No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2760.18394972040975466No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG2750.18328323591551643No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAGGTGGGAGGA2740.18261675142127817No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATCACTGGCCT2720.1812837824328017No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG2680.17861784445584872No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2660.17728487546737223No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA2620.17461893749041926No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACGCACAC2600.1732859685019428No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTCCTCAGCCACC2540.16928706153651335No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGGGGGAATGGGA2510.1672876080537986No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCTACGTGGAAATG2470.16462167007684567No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2390.15928979412293973No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCGCCTGTCACC2380.1586233096287015No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA2310.15395791816903381No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCTCATAGGCAG2260.1506254956978426No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCGGGAGTGCAGT2240.1492925267093661No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG2220.14795955772088962No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTCCTGCCTGTAAT2200.14662658873241316No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGGAAACAGCTATG2130.14196119727274545No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGGAAACAATGATG2090.13929525929579248No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT2080.13862877480155425No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGCACAGC2050.1366293213188395No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1980.13196392985917182No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGTGCAGT1920.12796502289374237No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAACAGGAAACAGAA1900.12663205390526588No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1890.12596556941102766No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC1820.12130017795135994No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG1780.118634239974407No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTTATATGCCTCCT1780.118634239974407No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA1770.11796775548016875No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCATTCAGAATCCG1720.11463533300897755No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTTTTTAAATGAG1700.11330236402050106No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGTCAGGTCCAAGT1690.11263587952626282No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGATCC1690.11263587952626282No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACAG1650.10996994154930985No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1580.10530455008964217No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC1580.10530455008964217No Hit
CAGGAAACAGCTATGACCTGCTGCTTTTCCTGCCAGAAGACAGAAGCCCA1580.10530455008964217No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGGAGGGCAGT1560.10397158110116568No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC1550.10330509660692745No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCAGAGGGCGGCCA1530.10197212761845097No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA1520.10130564312421271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)