FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124956
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC30152.4128493229616828No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA25472.0383174877556898No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC23421.8742597394282787No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT20441.6357757930791639No Hit
TGTAAAACGACGGCCAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG20371.6301738211850572No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC19731.5789557924389384No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG19721.5781555107397804No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC19681.5749543839431481No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG19621.5701526937481995No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG18581.4869233970357565No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG18151.4525112839719583No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC17931.4349050865904798No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC17741.419699734306476No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA17481.398892410128365No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC16601.3284676206024522No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA16441.3156631134159225No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT14471.1580076186817758No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC14461.157207336982618No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC13631.0907839559524952No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA13621.089983674253337No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT13191.0555715611895387No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC13001.0403662089055348No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG12981.0387656455072185No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT11900.9523352219981432No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG11500.9203239540318191No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC11180.8947149396587599No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG11120.8899132494638112No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC11010.8811101507730722No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG10870.8699062069848588No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG10800.864304235090752No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT10790.8635039533915938No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT10790.8635039533915938No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG10340.8274912769294792No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC10140.8114856429463172No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC10020.8018822625564198No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC9990.7994814174589455No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT9950.7962802906623132No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT9900.7922788821665225No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA9180.7346585998271392No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG8930.7146515573481865No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG8590.6874419795768111No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG8570.6858414161784948No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC8430.6746374723902814No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC8370.6698357821953328No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA8230.6586318384071194No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG8220.6578315567079612No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG8130.6506290214155382No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG8130.6506290214155382No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA8130.6506290214155382No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA7670.6138160632542655No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT7540.6034124011652101No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC7280.5826050769870994No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT7210.5770031050929927No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG7140.571401133198886No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG7060.5649988796056212No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA7040.563398316207305No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6900.5521943724190915No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA6450.5161816959569768No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG6360.508979160664554No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT6320.5057780338679215No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC6240.4993757802746567No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT6230.49857549857549854No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG6210.49697493517718233No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA6030.4825698645923365No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT5990.4793687377957041No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT5980.47856845609654597No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC5720.4577611319184353No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG5700.4561605685201191No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT5590.44735746982937996No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG5170.4137456384647396No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4760.38093408879925733No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA4100.3281154966548225No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG4040.32331380645987384No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3890.31130958097250233No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3880.3105092992733442No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC3450.27609718620954576No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA3070.2456864816415378No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3010.2408847914465892No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGCCCGGATGTTGG2930.23448253785332437No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2900.23208169275585008No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGTTGGGGTCCC2730.2184769038701623No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2720.2176766221710042No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG2660.2128749319760556No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2490.1992701430903678No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGCCCCCAAAGGCT2480.1984698613912097No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGGGTGCTTTCTCT2470.19766957969205162No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2450.19606901629373538No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG2440.1952687345945773No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2290.18326450910720574No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG2260.18086366400973142No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG2030.16245718492909506No Hit
TGTAAAACGACGGCCAGTTGTGGAAGTCGGGGACATAAGATGATTTTCAC1990.15925605813246263No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACACCTTC1940.1552546496366721No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTTAGCTA1900.15205352284003967No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT1750.14004929735266813No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA1700.1360478888568776No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG1580.12644450846698038No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC1560.12484394506866417No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT1450.11604084637792504No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC1440.11524056467876692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)