FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83625
Sequences flagged as poor quality0
Sequence length300
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA20342.432286995515695No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC17682.114200298953662No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCAGGCATTCAA16021.915695067264574No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT13781.6478325859491778No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC13761.645440956651719No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG13501.6143497757847534No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG13461.6095665171898355No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT13121.5689088191330345No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC11561.3823617339312406No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC11331.3548579970104633No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT10941.308221225710015No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC9831.1754857997010462No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT9641.152765321375187No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA9301.1121076233183858No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT8971.072645739910314No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT8601.0284005979073243No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA8240.9853512705530643No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC8210.9817638266068759No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG8120.9710014947683109No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC7460.8920777279521674No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT7130.8526158445440958No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG7100.8490284005979074No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG6960.8322869955156951No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT6750.8071748878923767No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGCCCAGTGTGTT6610.7904334828101643No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG6430.7689088191330344No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG6260.7485799701046337No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC6250.7473841554559043No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG6140.7342301943198805No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG6030.7210762331838565No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT6010.7186846038863977No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT5870.7019431988041853No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG5750.6875934230194319No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG5750.6875934230194319No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA5740.6863976083707025No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC5560.6648729446935725No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA5390.6445440956651719No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG4850.5799701046337817No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCACGTGCAGGA4840.5787742899850523No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT4840.5787742899850523No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCGGCAGCGACAT4840.5787742899850523No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG4800.5739910313901345No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCAGGTGACTCCAG4550.5440956651718983No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT4440.5309417040358745No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA4210.5034379671150971No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTGTGCAGGCG4060.48550074738415544No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC3990.47713004484304933No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG3970.47473841554559043No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC3900.46636771300448426No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC3800.45440956651718983No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGTGTAAAACTACG3750.4484304932735426No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC3730.4460388639760837No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG3700.44245142002989535No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG3560.42571001494768307No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT3540.4233183856502242No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC3480.41614349775784754No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG3320.39701046337817636No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC3280.3922272047832586No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG3120.37309417040358744No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGTGTGGCCTGAG3090.3695067264573991No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA3060.36591928251121075No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT3050.36472346786248133No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC3040.36352765321375186No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA2990.35754857997010464No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT2940.35156950672645737No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT2920.34917787742899853No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG2920.34917787742899853No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG2870.34319880418535126No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG2870.34319880418535126No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCTTCGACATACAA2850.34080717488789236No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT2820.33721973094170404No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2810.33602391629297457No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAGGAATTCAG2750.32884902840059793No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA2630.31449925261584455No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCAGCACCCGGCAGC2620.31330343796711513No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT2570.30732436472346786No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTGAGCGAGATAA2550.30493273542600896No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG2540.30373692077727954No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA2510.30014947683109117No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA2470.2953662182361734No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCACAAGGACACAG2430.2905829596412556No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT2420.28938714499252616No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCCCAGTCACC2350.28101644245142005No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT2330.27862481315396115No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG2240.2678624813153961No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT2210.26427503736920777No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTGTTCGGAGGCA2200.2630792227204783No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGAAACAACT2190.26188340807174887No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2180.26068759342301945No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG2120.25351270553064276No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT2080.248729446935725No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2020.24155455904334827No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA1990.23796711509715993No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGCCTCAGGGCTT1970.23557548579970106No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATTGAAAATCTGC1950.23318385650224213No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGGCCCAAGTCA1940.2319880418535127No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGCACGATTAGGG1920.2295964125560538No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1870.22361733931240657No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCAATAATGCTGCT1870.22361733931240657No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGGTGGAGTGTGT1830.21883408071748878No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1810.2164424514200299No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG1790.214050822122571No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG1790.214050822122571No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCAGAGGCTCACGAC1780.21285500747384153No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC1740.20807174887892377No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT1720.20568011958146487No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGGACTCCAG1700.20328849028400597No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1680.2008968609865471No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT1670.19970104633781766No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG1670.19970104633781766No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACTTCCCCTGCAC1660.19850523168908818No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCCCTCGTTCCC1650.19730941704035873No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1630.19491778774289983No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAAAACGACGG1600.19133034379671152No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGACACTGTGAGGCCC1590.19013452914798207No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAATTTTGG1570.18774289985052317No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA1530.18295964125560538No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTCCACA1520.18176382660687593No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGGAACAACT1480.17698056801195816No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTCCTGCTCA1470.17578475336322869No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCCTCAAGAGGT1440.17219730941704037No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTTCAGCCCAGGAG1430.17100149476831092No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG1430.17100149476831092No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGACCTAGTCCACG1400.16741405082212257No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGCTTGCAGCACA1380.16502242152466368No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATAGCT1310.15665171898355756No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT1290.15426008968609867No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGCGGTGCAATAGG1270.15186846038863977No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT1270.15186846038863977No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAAAACGACGGC1250.14947683109118087No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC1240.14828101644245142No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCACATTGGAGT1240.14828101644245142No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCTCCCCAGTGCC1230.14708520179372198No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGATCCTCCC1210.14469357249626308No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1190.14230194319880418No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGGGGACCGAGAGC1180.14110612855007473No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGCACTCCAGCTG1180.14110612855007473No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG1170.13991031390134528No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGATGGTGTGGC1110.13273542600896862No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCCTCT1110.13273542600896862No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGTAGTGC1110.13273542600896862No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGACCAACAATGGCCTG1090.13034379671150972No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG1090.13034379671150972No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG1090.13034379671150972No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGTGAAAACGACGG1070.12795216741405083No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGAGAGCGGTTCC1060.12675635276532138No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG1060.12675635276532138No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCGCCTCCTGCTCAGCC1060.12675635276532138No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGCACCACAGCCT1050.12556053811659193No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCTCAGTCACC1030.12316890881913303No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTAGCAGCTGGGAA1030.12316890881913303No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGTGTGGCCTGA1020.1219730941704036No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGAACCCTGGCCT1010.12077727952167414No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCCTGCTACGC1000.1195814648729447No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAAAGGGGC960.1147982062780269No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAGGGTCCTAAA960.1147982062780269No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA920.11001494768310913No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC910.10881913303437966No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCTACGGGATC870.10403587443946188No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCTAGC870.10403587443946188No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG860.10284005979073244No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGACATGAGGCAA850.10164424514200299No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC850.10164424514200299No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTAGGGACACGTA840.10044843049327355No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT840.10044843049327355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)