FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83625
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT18922.2624813153961134No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG15741.8822122571001496No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC15081.8032884902840058No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC12821.533034379671151No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC12671.5150971599402092No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG12671.5150971599402092No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC12641.511509715994021No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT12081.4445440956651718No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA12011.4361733931240657No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG11951.428998505231689No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC10611.2687593423019432No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC10401.2436472346786247No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT9351.1180866965620329No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA9031.0798206278026907No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC8611.029596412556054No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG8310.9937219730941703No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC8130.9721973094170404No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC7490.8956651718983558No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7370.8813153961136024No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC7110.8502242152466368No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG6670.7976083707025411No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG6420.7677130044843049No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC6350.7593423019431988No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6300.7533632286995516No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG6050.7234678624813153No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTGGGCAGGAAGAA6010.7186846038863977No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA5890.7043348281016443No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5890.7043348281016443No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA5380.6433482810164424No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC5330.6373692077727952No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG5320.6361733931240658No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG5240.6266068759342303No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5200.6218236173393124No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT4850.5799701046337817No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT4840.5787742899850523No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4630.5536621823617339No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG4560.5452914798206278No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCTCTCCACGTGC4550.5440956651718983No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCATCCTGCACGTGG4510.5393124065769805No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4300.5142002989536621No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4210.5034379671150971No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC4150.4962630792227205No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC4140.49506726457399103No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3830.45799701046337815No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC3780.45201793721973094No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3750.4484304932735426No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA3720.44484304932735425No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC3700.44245142002989535No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA3510.41973094170403585No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC3450.41255605381165916No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTTTACACAGAAC3430.41016442451420027No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG3420.4089686098654709No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3320.39701046337817636No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG3180.38026905829596414No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC3160.37787742899850524No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC3090.3695067264573991No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC3070.36711509715994023No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC2890.34559043348281016No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGCCTCCCGACT2850.34080717488789236No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC2850.34080717488789236No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTATGTC2780.33243647234678625No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA2780.33243647234678625No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA2740.32765321375186846No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG2740.32765321375186846No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT2700.3228699551569507No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG2700.3228699551569507No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA2690.3216741405082212No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG2690.3216741405082212No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG2690.3216741405082212No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT2650.31689088191330345No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2630.31449925261584455No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTGGGTGTCTGC2580.30852017937219733No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2550.30493273542600896No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGCTCAAGG2540.30373692077727954No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT2480.29656203288490285No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG2480.29656203288490285No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2420.28938714499252616No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC2350.28101644245142005No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG2340.27982062780269057No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTGGCAG2340.27982062780269057No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2280.2726457399103139No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCAGGGAAAG2240.2678624813153961No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2230.26666666666666666No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG2230.26666666666666666No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG2220.26547085201793724No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCCCACCGCAAA2160.25829596412556055No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2140.25590433482810165No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT2010.24035874439461885No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC2010.24035874439461885No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC2010.24035874439461885No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTGTTTCCTGCAG1870.22361733931240657No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1860.22242152466367712No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1850.22122571001494767No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1840.22002989536621825No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGCCAGGGT1830.21883408071748878No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAGATTTTCAAT1820.21763826606875933No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT1780.21285500747384153No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTATTGAGGCACTT1780.21285500747384153No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1760.21046337817638266No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1730.20687593423019432No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG1660.19850523168908818No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC1660.19850523168908818No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA1630.19491778774289983No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCAGGAAGA1630.19491778774289983No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCAGGGGCCACGC1600.19133034379671152No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT1590.19013452914798207No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCGTGAGCCTCTGG1560.18654708520179372No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTTATCCC1530.18295964125560538No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1520.18176382660687593No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1520.18176382660687593No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT1500.17937219730941703No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT1490.17817638266068758No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT1490.17817638266068758No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGTGCAGGGGAAG1490.17817638266068758No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA1470.17578475336322869No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1470.17578475336322869No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGAACGAGGGCCAGT1450.1733931240657698No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGTCCTGTGCCCA1430.17100149476831092No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTCCCCACCTCCT1420.16980568011958147No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT1400.16741405082212257No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG1360.16263079222720478No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTGTTCCCTGCAG1360.16263079222720478No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGGCTGTGCACCT1350.16143497757847536No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGTTGAT1300.1554559043348281No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC1290.15426008968609867No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGGTGACCCTAATC1270.15186846038863977No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTAATCTCGAG1260.15067264573991032No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTACTGG1240.14828101644245142No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA1240.14828101644245142No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGCACCGCACCCCT1220.14588938714499253No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG1220.14588938714499253No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCTTGGCCTGCT1170.13991031390134528No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCTCCAGAACAC1160.13871449925261584No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCCAGCTGGA1120.13393124065769804No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGGATAAGATGTG1110.13273542600896862No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC1090.13034379671150972No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCTAGGCTGTG1060.12675635276532138No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCACGTACTGGCAGG1050.12556053811659193No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG1030.12316890881913303No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGTCACTGCAGCCTGGG1010.12077727952167414No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCATCCTGCCTCC1000.1195814648729447No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCGTCGTTTTC1000.1195814648729447No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1000.1195814648729447No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCGGGTTCCCA980.1171898355754858No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTATGACCGCCCACC980.1171898355754858No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT970.11599402092675636No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG970.11599402092675636No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA960.1147982062780269No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCGGGCATCCT960.1147982062780269No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCATCTGCCCCTT950.11360239162929746No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCAGAGGCCAG930.11121076233183856No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTGCCTCCCGAC920.11001494768310913No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG910.10881913303437966No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTTTCA900.10762331838565023No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA880.10523168908819133No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCCC860.10284005979073244No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC850.10164424514200299No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTAGCTATGACCGCC840.10044843049327355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)