FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94849
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA4640.4891986209659564No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA3680.3879851131798965No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT3300.34792143301458106No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT3280.34581281826903815No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3240.3415955887779523No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2680.2825543759027507No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2660.2804457611572078No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2480.26146822844732154No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2430.2561966915834642No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA2390.2519794620923784No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2340.24670792522852114No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2330.24565361785574966No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2280.24038208099189237No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2200.2319476220097207No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC2160.22773039251863492No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2130.22456747040032052No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT2110.22245885565477758No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT2080.21929593353646323No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2040.2150787040453774No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2040.2150787040453774No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2000.21086147455429155No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1950.20558993769043427No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1900.20031840082657698No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1880.19820978608103404No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1870.1971554787082626No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1840.19399255658994824No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1830.19293824921717678No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1820.19188394184440533No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1770.18661240498054804No Hit
NNGGANNNNGNTATGNNNACACATACTCCCTTGCTCCAGGGCTGCAGAAC1720.18134086811669076No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1710.18028656074391927No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1700.17923225337114781No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1610.1697434870162047No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1600.16868917964343325No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1560.16447195015234742No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1560.16447195015234742No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1560.16447195015234742No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1550.16341764277957596No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1530.16130902803403305No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1530.16130902803403305No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1520.1602547206612616No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1520.1602547206612616No Hit
NNGGANNNNGNTATGANNACACATACTCCCTTGCTCCAGGGCTGCAGAAC1520.1602547206612616No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1520.1602547206612616No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1500.15814610591571868No Hit
NAGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1490.15709179854294722No Hit
NNGGANNNNGNTATGNNNCACCGCTGTCCCTTCCCAAGGACTATGCTGAA1480.15603749117017576No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1480.15603749117017576No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1480.15603749117017576No Hit
NAGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1460.15392887642463282No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1440.1518202616790899No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1420.14971164693354702No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1420.14971164693354702No Hit
NAGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1410.14865733956077554No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA1390.14654872481523262No Hit
NAGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1390.14654872481523262No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1390.14654872481523262No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1370.1444401100696897No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1310.13811426583306097No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1300.1370599584602895No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1290.13600565108751805No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1280.1349513437147466No Hit
NAGGANNNNGNTATGNNNACACATACTCCCTTGCTCCAGGGCTGCAGAAC1260.13284272896920368No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1260.13284272896920368No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1260.13284272896920368No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1250.13178842159643223No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1240.13073411422366077No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1230.1296798068508893No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1210.1275711921053464No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1200.12651688473257494No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1190.12546257735980346No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1170.12335396261426057No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1170.12335396261426057No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1170.12335396261426057No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1160.1222996552414891No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1160.1222996552414891No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1140.12019104049594619No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1140.12019104049594619No Hit
NAGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1140.12019104049594619No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1120.11808242575040327No Hit
NNGGANNNNGNTATGANNCACCGCTGTCCCTTCCCAAGGACTATGCTGAA1120.11808242575040327No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1120.11808242575040327No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1110.11702811837763182No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1090.1149195036320889No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1090.1149195036320889No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1070.11281088888654599No Hit
NAGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1060.11175658151377453No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACAGGGGCGATAGTG1050.11070227414100306No Hit
NNGGANNNNGNTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1040.10964796676823162No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1030.10859365939546015No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1020.1075393520226887No Hit
NAGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1010.10648504464991723No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1010.10648504464991723No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1010.10648504464991723No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA1000.10543073727714578No Hit
NAGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA980.10332212253160286No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA970.10226781515883142No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA970.10226781515883142No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC960.10121350778605995No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT950.10015920041328849No Hit
NAGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC950.10015920041328849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)