FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185537
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG98535.3105310531053105No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA68023.6661151145054625No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC54342.928795873599336No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC49612.673860200391297No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA49042.6431385653535413No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC46242.4922252704312347No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG45452.4496461622210126No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT42112.2696281604208326No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT41122.2162695311447314No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA39692.139195955523696No Hit
TGTAAAACGACGGCCAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG37772.035712553291257No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA34471.857850455704253No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG33781.8206611080269706No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT29621.5964470698566862No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT28541.5382376561009394No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGAGTAGCCACAG22451.2100012396449227No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC22441.2094622635916286No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCCAAGTCACCAGC19001.0240545012585092No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG17850.9620722551297046No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG16950.9135644103332489No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC16230.8747581344960844No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT14670.7906778701822279No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA13410.7227668874671899No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC11970.6451543357928607No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA10960.5907177544101716No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGACCAAGCAGGATC10750.5793992572909985No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGGCAATCATGGCT8660.4667532621525626No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG8350.45004500450045004No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCTCCCGGGTTCA7790.4198623455159888No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGATGGTGCTCTGGT7770.4187843934094008No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC7050.37997811757223626No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA6860.3697375725596512No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC6770.36488678808000563No Hit
TGTAAAACGACGGCCAGTCAGACCTTTAAGAGTATGCTTTATTGAGCTTG6650.3584190754404782No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT6600.35572419517400844No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCATTGGTGCACTGA6400.34494467410812935No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG6400.34494467410812935No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGGGGAGGTG6330.3411718417350717No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG5930.3196127996033136No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC5660.30506044616437694No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA5620.30290454195120115No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG5450.29374194904520395No Hit
TGTAAAACGACGGCCAGTCAGACCTTTAAGAGTATGCTTTATTGAGCAGG5430.29266399693861606No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT3960.21343451710440506No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT3880.20912270867805344No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC3790.20427192419840787No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA3760.202654996038526No Hit
TGTAAAACGACGGCCAGTTCCCAAGTTCGCTTTGCATCTTTGAAGAGCCC3740.2015770439319381No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGAGCCCCAATCCTGTC3670.19780421155888042No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC3460.18648571443970746No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT3460.18648571443970746No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG3440.18540776233311954No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACAGTCATAGT3280.17678414548041632No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG3270.17624516942712234No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCCCTTGCAGAGG3230.17408926521394655No Hit
TGTAAAACGACGGCCAGTCAGACCTTTAAGAGTATGCTTTATTGAGACAG3070.1654656483612433No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG3010.16223179204147958No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG2960.15953691177500984No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG2940.15845895966842194No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2930.157919983615128No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGTAATCGATAAAC2810.15145227097560057No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCAACATCCGCTTC2790.15037431886901265No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG2710.14606251044266103No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2630.14175070201630943No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCCAGGAGGCCTCT2590.1395947978031336No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGTGTGATCATGGC2580.13905582174983966No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGTTCTCAGAGC2470.1331270851636062No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCAGCAACTATCC2440.13151015700372432No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCCACAAGGAGACGATG2440.13151015700372432No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGAGCTCGGGGAAGA2330.12558142041749085No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2330.12558142041749085No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCATAGCTCACC2240.12073063593784528No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTAACC2150.11587985145819972No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG2090.112645995138436No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA2050.11049009092526019No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGAAGTCCCCACACA2040.10995111487196622No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA2040.10995111487196622No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGAAACACTA2040.10995111487196622No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGAGAGGGAATAAAG1980.10671725855220253No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCAGGTGTGGACGC1920.1034834022324388No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATGATGGCTCACT1910.10294442617914486No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA1910.10294442617914486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)