FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185537
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT51042.7509337760123316No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT48972.6393657329804836No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG31171.679988358117249No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC24601.3258810911031222No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG23381.2601260126012601No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT21801.1749677961808158No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC20571.1086737416256596No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT19011.024593477311803No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT18681.0068072675531026No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT18310.9868651535812264No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT17270.9308116440386554No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT16980.9151813384931308No Hit
CAGGAAANAGCTATGANNAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG16810.9060187455871336No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT13200.7114483903480169No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC12860.6931232045360225No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT12220.6586287371252095No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG11180.6025752275826385No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG9690.5222677956418396No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTTGGGGTGGATC9430.5082544182561969No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8600.4635194058327989No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8550.46082452556632914No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT7860.42363517788904637No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT7790.4198623455159888No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG7650.4123166807698734No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT7510.40477101602375803No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT7160.38590685415846976No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT7000.3772832373057665No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6540.3524903388542447No Hit
CAGGAAANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC6430.34656160226801125No Hit
CAGGAAANNGCTATGANNAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG6110.32931436856260476No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG6060.32661948829613496No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA5680.3061383982709648No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5390.2905080927254402No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT5360.28889116456555836No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5240.2824234519260309No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5230.28188447587273696No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT5210.28080652376614906No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT5070.2732608590200337No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT5010.27002700270027No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4960.2673321224338003No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4900.2640982661140366No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT4890.2635592900607426No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4720.2543966971547454No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC4440.23930536766251476No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4410.2376884395026329No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGGGCCCAGATCC4380.23607151134275103No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGGGCAAGAGAATG3980.21451246921099296No Hit
CAGGAAANAGCTATGANNAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG3970.21397349315769898No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3920.21127861289122923No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCCCTTGAATCTA3900.21020066078464134No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCATCCCCACCTCC3710.19996011577205625No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT3630.19564830734570463No Hit
CAGGAAANNGCTATGANNAGGTCCAGATGGCTGGTGACTTGGGGTGGATC3630.19564830734570463No Hit
CAGGAAANAGCTATGANNCTGTCCCAAGGGAGCAAGCTCAATAAAGCATA3540.19079752286605905No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3420.18432981022653164No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3390.1827128820666498No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG3380.1821739060133558No Hit
CAGGAAANNGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT3370.18163492996006186No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3270.17624516942712234No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3220.1735502891606526No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3160.17031643284088888No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3130.16869950468100703No Hit
CAGGAAANAGCTATGANNCTGTCCCAAGGGAGCAAGCCAGGAAACAGCTA3060.16492667230794936No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3010.16223179204147958No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2980.16061486388159774No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT2910.15684203150854006No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2900.15630305545524611No Hit
NAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2860.15414715124207032No Hit
CAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2820.15199124702889452No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2800.1509132949223066No Hit
NAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2790.15037431886901265No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCTCCCCACCTCCC2750.14821841465583685No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2730.14714046254924892No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2690.14498455833607313No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2670.1439066062294852No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2660.14336763017619128No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2660.14336763017619128No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2650.14282865412289733No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2640.14228967806960338No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2640.14228967806960338No Hit
CAGGAAACAGCNATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2610.1406727499097215No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2600.14013377385642756No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2590.1395947978031336No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2540.13689991753666383No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2500.13474401332348804No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2460.13258810911031224No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCCTCTTGGAATC2450.1320491330570183No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2450.1320491330570183No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2420.13043220489713642No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2410.12989322884384247No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2330.12558142041749085No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2320.1250424443641969No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2320.1250424443641969No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2290.12342551620431505No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2290.12342551620431505No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2250.12126961199113923No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2180.11749677961808158No Hit
NAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2120.11426292329831786No Hit
CAGGAAANNGCTATGANNAGGCTTTGTGTGTGGGGACTGGGCCCAGATCC2100.11318497119172995No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGCTTGAGCCCAG2100.11318497119172995No Hit
NAGGANANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2090.112645995138436No Hit
CAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2090.112645995138436No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2090.112645995138436No Hit
NAGGAAANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2060.11102906697855414No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2060.11102906697855414No Hit
NAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2020.10887316276537834No Hit
CAGGANANNGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1970.10617828249890858No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1970.10617828249890858No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGTACC1960.10563930644561462No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1950.10510033039232067No Hit
CAGGAAANAGCTATGANNATATGACAACTTGAACACATCTGACATACAGA1950.10510033039232067No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1940.10456135433902672No Hit
CAGGANANNGCTATGANNAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG1930.10402237828573277No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG1920.1034834022324388No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1880.101327498019263No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1860.10024954591267511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)