FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88084
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT6110.6936560555833069No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4210.47795286317605923No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3880.4404886244948004No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2960.33604286817129103No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2860.3246900685709096No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2830.32128422869079515No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2820.320148948730757No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2660.30198446937014667No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2630.2985786294900322No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2580.2929022296898415No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2500.2838199900095364No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2330.26452023068888786No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2200.249761591208392No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2180.24749103128831573No Hit
NNGGANNNNGNTATGNNNTGCCAAAATTAAGGATGCACACCTGGAGTCAC2040.23159711184778164No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2030.23046183188774352No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2000.22705599200762908No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1980.2247854320875528No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1890.21456791244720946No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1840.20889151264701875No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1830.20775623268698062No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1790.203215112846828No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1770.20094455292675173No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1730.19640343308659916No Hit
NNGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG1720.195268153126561No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1720.195268153126561No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1710.19413287316652286No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1700.1929975932064847No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1670.18959175332637027No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1650.187321193406294No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1640.18618591344625585No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1630.1850506334862177No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1610.1827800735661414No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1590.18050951364606513No Hit
NNGGANNNNGNTATGANNTGCCAAAATTAAGGATGCACACCTGGAGTCAC1570.17823895372598883No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1560.17710367376595068No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1550.17596839380591253No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1530.17369783388583626No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1520.1725625539257981No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1510.17142727396575994No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1510.17142727396575994No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1490.16915671404568366No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1460.16575087416556925No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1460.16575087416556925No Hit
NAGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1380.15666863448526408No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1360.15439807456518778No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1360.15439807456518778No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1330.15099223468507333No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1320.14985695472503519No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1300.1475863948049589No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1300.1475863948049589No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1300.1475863948049589No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1250.1419099950047682No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1240.14077471504473002No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1230.13963943508469187No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1230.13963943508469187No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1200.13623359520457745No Hit
NNGGANNNNGNTATGANNGATGGCAGAATGGAAACATGGCAGCTGCCTTG1200.13623359520457745No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1200.13623359520457745No Hit
NNGGANNNNGNTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1190.1350983152445393No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1190.1350983152445393No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1190.1350983152445393No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1170.132827755324463No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1140.12942191544434856No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1130.12828663548431044No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1130.12828663548431044No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1110.12601607556423414No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG1100.124880795604196No Hit
NNGGANNNNGNTATGANNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1090.12374551564415787No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1080.12261023568411969No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1080.12261023568411969No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1080.12261023568411969No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1060.12033967576404342No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1060.12033967576404342No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1020.11579855592389082No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1020.11579855592389082No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1020.11579855592389082No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1010.11466327596385269No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1010.11466327596385269No Hit
NAGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1000.11352799600381454No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1000.11352799600381454No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG990.1123927160437764No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG990.1123927160437764No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC980.11125743608373824No Hit
NAGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG980.11125743608373824No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC980.11125743608373824No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGATTCTCAGATTCC980.11125743608373824No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC960.10898687616366196No Hit
NAGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC960.10898687616366196No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC950.10785159620362382No Hit
NNGGANNNNGNTATGNNNGTTTTCACTGGCTCCCGGGGCAGCCGGACGAG950.10785159620362382No Hit
NAGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT950.10785159620362382No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT950.10785159620362382No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCGTCGTTTTC950.10785159620362382No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA940.10671631624358567No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT940.10671631624358567No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC930.10558103628354754No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT930.10558103628354754No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG920.10444575632350937No Hit
NAGGANNNNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT920.10444575632350937No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG920.10444575632350937No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGATCCTCAGATTCC910.10331047636347122No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT900.10217519640343309No Hit
NNGGANNNNGNTATGNNNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG900.10217519640343309No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC890.10103991644339494No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG890.10103991644339494No Hit
NNGGANNNNGNTATGANNGTTTTCACTGGCTCCCGGGGCAGCCGGACGAG890.10103991644339494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)