FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108855
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC32773.010426714436636No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC31932.9332598410729873No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC30022.7577970694961187No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG27632.5382389417114513No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG27052.48495705296036No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG26182.405034219833724No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA25782.368288089660558No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC24932.2902025630425795No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC22492.066051168986266No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA20941.9236599145652473No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG20151.8510863074732442No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT17651.6214229938909557No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT17121.572734371411511No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC16171.4854623122502413No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC15621.4349363832621376No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG15551.4285058104818336No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG13501.2401818933443571No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT13251.2172155619861282No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG11871.0904414128887052No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG11691.0739056543107803No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA11681.0729870010564513No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG11511.0573698957328557No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG10460.9609113040282944No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC10410.9563180377566488No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT10210.9379449726700656No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT10170.9342703596527492No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC10090.9269211336181159No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC10050.9232465206007993No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC9810.9011988424968996No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC9770.897524229479583No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC9580.880069817647329No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT9480.8708832851040375No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC9440.8672086720867209No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT9330.8571034862891002No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG8550.7854485324514262No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA8360.7679941206191723No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT7890.7248174176657021No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG7830.7193054981397271No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7260.6669422626429654No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG7140.6559184235910156No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT6930.6366267052501033No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA6890.6329520922327868No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT6530.5998805750769373No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC6280.5769142437187084No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC6050.5557852188691379No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT5690.5227137017132883No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC5530.5080152496440219No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC5390.49515410408341376No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG4700.43176702953470214No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA4670.42901106977171466No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG4550.41798723071976485No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG4440.4078820449221442No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG4290.39410224610720684No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA4260.3913462863442194No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG4160.38215975380092787No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT4130.3794037940379404No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC3890.3573561159340407No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA3870.3555188094253824No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGGAGGATCCTCC3740.3435763171191034No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT3460.3178540259978871No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG3450.31693537274355793No Hit
TGTAAAACGACGGCCAGTTGTGGAAGTCGGGGACATAAGATGATTTTCAC3370.3095861467089247No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2810.2581415644664921No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2750.2526296449405172No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCCGTGGAGGTGG2700.24803637866887143No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGAGGATCCTCCC2640.24252445914289653No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT2610.23976849937990904No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC2490.2287446603279592No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA2480.22782600707363007No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2430.2232327408019843No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG2340.2149648615130219No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2330.21404620825869275No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCTGGGATTCCTCT2190.20118506269808462No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCATTGTCACA2060.18924257039180561No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC2000.1837306508658307No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1780.16352027927058932No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGAAAACAATCAGATG1770.16260162601626016No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACGACAGCGCCTTC1700.1561710532359561No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCCCCAGGCAGT1690.15525239998162693No Hit
TGTAAAACGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA1590.14606586743833538No Hit
TGTAAAACGACGGCCAGTCTGCTGCTGCCCACTGAGATTTCTAAGCAGTA1460.1341233751320564No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT1460.1341233751320564No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAAAAGGTG1410.12953010886041064No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGAACCTGGGA1380.12677414909742318No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1230.11299435028248588No Hit
TGTAAAACGACGGCCAGTGCACTGTGGGCTTTTATTGCCATGAGCTAGAA1180.10840108401084012No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG1160.1065637775021818No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGAGAAGGGGC1120.10288916448486518No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCAGGCGCCTG1110.10197051123053605No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1090.10013320472187773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)