FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121358
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA33272.7414756340743915No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC26842.2116382933139964No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG25712.118525354735576No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG25452.097101138779479No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC24782.0418925822772294No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT23711.9537236935348308No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC22361.842482572224328No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG21111.7394815339738623No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT18951.561495739877058No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC17461.438718502282503No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG16201.3348934557260337No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC15871.3077011816279107No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA15691.2928690321198437No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA15531.2796848992237841No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC14861.2244763427215346No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG14691.2104682015194714No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC14371.184099935727352No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC14221.1717398111372963No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13851.1412515038151585No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC13781.1354834456731324No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC13731.1313634041431138No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC13501.112411213105028No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC13431.106643154963002No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC13031.073682822722853No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG12601.038250465564693No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12551.0341304240346743No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT12551.0341304240346743No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG12251.0094101748545625No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA12020.9904579838164769No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT11730.9665617429423689No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC11710.9649137263303613No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT11630.9583216598823316No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC11600.9558496349643205No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT11030.9088811615221081No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG10540.8685047545279257No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA10350.8528485967138548No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC10150.8363684305937804No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA8550.7045271016331844No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC7830.6451985036009164No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG7760.6394304454588902No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC7660.631190362398853No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC7320.6031740799947264No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC7300.601526063382719No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC6980.5751577975905997No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC6920.5702137477545773No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT6820.5619736646945401No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG6820.5619736646945401No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA6680.550437548410488No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG6630.5463175068804693No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6420.5290133324543912No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT6180.5092371331103017No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG5950.4902849420722161No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5920.4878129171542049No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT5920.4878129171542049No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC5900.48616490054219746No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG5870.48369287562418634No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAGCCGAAGCTGT5760.47462878425814536No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA5630.4639166762800969No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC5620.46309266797409315No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA5510.4540285766080522No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT5500.45320456830204847No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT5430.4474365101600224No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA5410.445788493548015No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA5410.445788493548015No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA5400.44496448524201127No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT5230.43095634403994787No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT5180.4268363025099293No Hit
TGTAAAACGACGGCCAGTCAGTGTCCGCAGAAGCAAGGGGCTGAAAAGAT4930.40623609485983625No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG4850.39964402841180635No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4760.39222795365777285No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC4730.3897559287397617No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT4370.36009162972362757No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC4330.35679559649961273No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT4320.35597158819360897No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG4240.34937952174557924No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA4240.34937952174557924No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA3730.3073550981393892No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG3670.3024110483033669No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT3540.29169894032531846No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTGGGCTCACT3450.28428286557128496No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT3360.27686679081725146No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3260.2686267077572142No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA3210.2645066662271956No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA3190.2628586496151881No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT3080.25379455824914715No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT2600.21424215956096837No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2560.2109461263369535No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT2500.2060020765009311No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2420.19941001005290135No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2390.1969379851348902No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGTTGGGGTCCC2330.1919939352988678No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC2300.18952191038085664No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC1900.15656157814070767No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1870.1540895532226965No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACACCTTC1850.15244153661068904No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTTAGCTA1770.14584947016265923No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1700.14008141202063318No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT1700.14008141202063318No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCTTAAGTATGC1650.13596137049061455No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC1630.1343133538786071No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT1560.12854529573658102No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAGCTCTCCAGAG1560.12854529573658102No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG1540.12689727912457358No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1530.12607327081856987No Hit
TGTAAAACGACGGCCAGTTTTCCCCTTCTCCCTCCAGGGGTGCTTTCTCT1460.12030521267654377No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1460.12030521267654377No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1310.10794508808648791No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)