FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121358
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT33072.724995467954317No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC27362.25448672522619No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT25962.139125562385669No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG25562.10616523014552No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT25312.0855650224954267No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG25242.0797969643534007No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC24802.0435405988892366No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC24302.0023401835890504No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT24241.9973961337530284No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT23391.9273554277427118No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG21581.7782099243560376No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA20041.651312645231464No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT18011.484038959112708No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA16771.3818619291682461No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA15431.2714448161637468No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG15191.2516686168196576No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA15141.247548575289639No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA14361.1832759274213482No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT14251.1742118360553075No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC14031.1560836533232255No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG13321.0975790635969611No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA13231.0901629888429276No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT12080.9954020336524992No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG11770.9698577761663838No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG10910.8989930618500634No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT10720.8833369040359926No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT10580.8718007877519406No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC9950.8198882644737058No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG9720.8009360734356202No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT9200.7580876415234266No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT8250.6798068524530727No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT7800.6427264786829051No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC7310.6023500716887226No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC7100.5850458972626444No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT7050.5809258557326258No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT7040.5801018474266221No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA6850.5644456896125514No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG6760.5570296148585178No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA6730.5545575899405065No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6640.5471415151864731No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA6370.5248932909243725No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC6200.5108851497223091No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC6130.5051170915802831No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA6030.49687700852024586No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT6010.49522899190823844No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG6010.49522899190823844No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG5860.48286886731818257No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA5810.4787488257881639No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC5650.46556469289210434No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA5580.45979663475007826No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA5520.45485258491405595No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA5480.45155655169004105No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT5460.44990853507803363No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5450.44908452677202987No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG5430.4474365101600224No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG5420.44661250185401874No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA5370.4424924603240001No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT5160.42518828589792185No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA5030.41447617791987346No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT4960.4087081197778474No Hit
CAGGAAACAGCTATGACCGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG4930.40623609485983625No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC4740.39057993704576544No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4710.38810791212775425No Hit
CAGGAAACAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT4640.3823398539857282No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT4470.36833171278366483No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT4460.3675077044776611No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG4430.36503567955965No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC4200.3460834885215643No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT4050.3337233639315084No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA3940.32465927256546745No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA3840.31641918950543024No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC3830.3155951811994265No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3770.3106511313634041No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT3620.2982910067733483No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT3590.29581898185533706No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC3530.29087493201931475No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA3400.28016282404126636No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC3400.28016282404126636No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCAGACGGC3330.2743947658992403No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA3250.2678026994512105No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT3070.25297054994314344No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA3060.25214654163713973No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT3050.25132253333113597No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC2990.2463784834951136No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC2900.23896240874108013No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2850.2348423672110615No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAGAAGAG2750.22660228415102424No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2630.21671418447897955No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCTCAGGTGATCT2600.21424215956096837No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG2580.21259414294896092No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2460.20270604327691624No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2360.19446596021687898No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2340.19281794360487153No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGGGATGAGGT2280.1878738937688492No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2270.18704988546284548No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2040.1680976944247598No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAAAGAGG1940.15985761136472254No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1910.15738558644671138No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGCAGAAGGG1840.1516175283046853No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA1810.14914550338667415No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCAGGCAGCTCCAC1780.14667347846866297No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1760.14502546185665552No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1650.13596137049061455No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCCACTCCTCCCCC1560.12854529573658102No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1550.12772128743057728No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1500.12360124590055867No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1500.12360124590055867No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC1490.12277723759455494No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCAGGCGCCGCGGG1370.11288913792251026No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1370.11288913792251026No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT1370.11288913792251026No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT1340.11041711300449908No Hit
CAGGAAACAGCTATGACCTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT1290.10629707147448046No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCACTGCAGCT1280.10547306316847675No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGCTCCGTGCTCT1250.10300103825046555No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC1250.10300103825046555No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1230.10135302163845812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)