FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003246634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26311
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4891.8585382539622213No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3231.2276234274637985No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1950.741134886549352No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1310.49789061609212876No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1300.49408992436623467No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC910.3458629470563643No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC750.28505187944205845No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG740.28125118771616436No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC720.27364980426437613No Hit
NAGGAANNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG710.269849112538482No Hit
NAGGANNNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG710.269849112538482No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT690.2622477290866938No Hit
NNGGAAANNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG660.2508456539090115No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC610.23184219527954086No Hit
NAGGANNNAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG610.23184219527954086No Hit
NNGGANNNNGNNATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG600.22804150355364675No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG590.22424081182775266No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC570.21663942837596442No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT570.21663942837596442No Hit
NNGGANANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG540.20523735319828207No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT530.201436661472388No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT530.201436661472388No Hit
NAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG520.19763596974649386No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC510.19383527802059974No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT510.19383527802059974No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC500.19003458629470563No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT490.18623389456881154No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC480.18243320284291742No Hit
NNGNNNNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG480.18243320284291742No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC480.18243320284291742No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC440.16723043593934098No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA440.16723043593934098No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC440.16723043593934098No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA430.16342974421344683No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT420.15962905248755274No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA400.1520276690357645No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG400.1520276690357645No Hit
NAGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC390.1482269773098704No Hit
NNGGAAANNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC390.1482269773098704No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT380.14442628558397627No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA370.14062559385808218No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG370.14062559385808218No Hit
NAGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC360.13682490213218806No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA360.13682490213218806No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT350.13302421040629395No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA350.13302421040629395No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG340.12922351868039983No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT340.12922351868039983No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA340.12922351868039983No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC330.12542282695450574No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT330.12542282695450574No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC320.12162213522861161No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC320.12162213522861161No Hit
NAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT320.12162213522861161No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT320.12162213522861161No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC320.12162213522861161No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT320.12162213522861161No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT320.12162213522861161No Hit
NNGGAAANNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC320.12162213522861161No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC320.12162213522861161No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA320.12162213522861161No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC310.1178214435027175No Hit
NNGGAAANNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT310.1178214435027175No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT310.1178214435027175No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT300.11402075177682337No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT300.11402075177682337No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC290.11022006005092927No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT290.11022006005092927No Hit
NAGGAANNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC290.11022006005092927No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC280.10641936832503517No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC270.10261867659914103No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA270.10261867659914103No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC270.10261867659914103No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA270.10261867659914103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)