FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128317
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC34242.6683915615234146No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC33602.6185150837379303No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC31592.4618717706928934No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT30722.394070933703251No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT30252.357442895329535No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT27882.1727440635301636No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA27812.167288823772376No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA27432.137674665087245No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA25541.9903831916269863No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT24681.9233616746027415No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC24511.9101132351909724No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT23481.8298432787549586No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC22641.7643804016615101No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG20851.6248821278552334No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT20801.6209855280282424No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG19551.5235705323534683No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT19471.5173359726302829No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA19201.4962943335645316No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT17951.3988793378897575No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC16841.312374821730558No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT16381.276526103322241No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT15311.1931388670246343No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC14871.1588487885471137No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC13891.0824754319380907No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT13651.0637717527685342No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT13501.0520819532875612No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG13331.038833513875792No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG13121.0224677946024299No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG13001.0131159550176516No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT12750.9936329558826968No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT11450.8923213603809316No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG11450.8923213603809316No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC10630.8284171232182798No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT10010.7800992853635917No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA9460.7372366872666911No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC9070.7068432086161616No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC9070.7068432086161616No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT8740.6811256497580211No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA8730.680346329792623No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA8270.6444976113843061No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT8130.6335871318687314No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT7850.611766172837582No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG7770.6055316131143964No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT7440.579814054256256No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC7240.5642276549482921No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC6830.5322755363669661No Hit
CAGGAAACAGCTATGACCGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG6730.5244823367129842No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA6510.507337297474224No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6350.49486817802785293No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG6250.487074978373871No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5980.4660333393081197No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA5870.45746081968873964No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA5720.4457710202077667No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA5650.4403157804499794No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA5570.43408122072679384No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT5440.42395006117661727No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG4390.342121464809807No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT4380.3413421448444088No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC3940.30705206636688825No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3660.28523110733573886No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA3630.28289314743954425No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT3580.27899654761255327No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT3530.2750999477855623No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAAGGAAAATGGAGA3370.2626308283391912No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC3370.2626308283391912No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA3340.26029286844299665No Hit
CAGGAAACAGCTATGACCTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT3310.2579549085468021No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA3180.24782374899662554No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3020.23535462955025446No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2950.22989938979246707No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC2930.22834074986167072No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC2890.22522347000007792No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC2780.2166509503806978No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC2750.21431299048450322No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCCACTCCTCCCCC2740.21353367051910505No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2550.19872659117653935No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA2530.197167951245743No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCTCAGGTGATCT2480.193271351418752No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGGGATGAGGT2470.1924920314533538No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCACTGCAGCT2360.18391951183397368No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT2310.1800229120069827No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCAGGCGCCGCGGG2280.17768495211078814No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2260.17612631217999175No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCAGGCAGCTCCAC2220.17300903231839895No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC2120.16521583266441703No Hit
CAGGAAACAGCTATGACCGCCATGCTTGAATCTTCGCAGTTTAGCTGTAA2070.16131923283742605No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA2020.1574226330104351No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCACAGGTCCCACCC1920.14962943335645318No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGCAGAAGGG1900.14807079342565677No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1900.14807079342565677No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTGTGGGGGAGCC1870.14573283352946217No Hit
CAGGAAACAGCTATGACCGCAGTGACAGGGCAAGGAGGGCCACAGTGGAA1820.14183623370247123No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG1740.1356016739792857No Hit
CAGGAAACAGCTATGACCACACAGCAACATACCGTTTGTAAACAATAAAA1730.13482235401388748No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT1570.12235323456751639No Hit
CAGGAAACAGCTATGACCACAGCTTTCTGAGGGTTGTTCCATATAGAACA1550.12079459463672002No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGCCACTGTGGA1550.12079459463672002No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT1420.11066343508654349No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT1320.10287023543256156No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1320.10287023543256156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)