FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79604
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6180.7763428973418421No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA4500.565298226219788No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4490.5640420079392995No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4300.5401738606100196No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3340.4195769056831315No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3290.4132958142806894No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3270.41078337771971263No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3030.3806341389879906No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2670.33541028089040753No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2530.3178232249635697No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2470.31028591528063915No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2460.3090296970001507No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2350.2952112959147781No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2290.28767398623184764No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2280.28641776795135926No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2270.2851615496708708No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2190.27511180342696345No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2170.2725993668659866No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2170.2725993668659866No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2080.2612934023415909No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2060.25878096578061405No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2060.25878096578061405No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2020.2537560926586604No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2020.2537560926586604No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2000.2512436560976835No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1980.24873121953670668No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT1950.24496256469524144No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1910.2399376915732878No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1870.2349128184513341No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1830.22988794532938042No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1730.21732576252449626No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1720.21606954424400782No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1720.21606954424400782No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1710.21481332596351943No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1700.21355710768303102No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1690.21230088940254258No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1620.20350736143912368No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1580.19848248831717No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1550.19471383347570473No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1530.1922013969147279No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1510.18968896035375107No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1490.18717652379277422No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1480.1859203055122858No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1460.18340786895130898No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1450.18215165067082056No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1380.17335812270740164No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1380.17335812270740164No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1370.17210190442691323No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1360.17084568614642479No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1350.16958946786593637No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1350.16958946786593637No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1340.16833324958544796No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1340.16833324958544796No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1330.16707703130495957No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1310.16456459474398272No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1310.16456459474398272No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1310.16456459474398272No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1300.1633083764634943No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1290.16205215818300586No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1260.15828350334154062No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1260.15828350334154062No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1240.1557710667805638No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1240.1557710667805638No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1240.1557710667805638No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1220.15325863021958694No Hit
NAGGAANNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1210.15200241193909855No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1200.15074619365861014No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1090.13692779257323753No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1080.13567157429274912No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1070.1344153560122607No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1060.13315913773177226No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1060.13315913773177226No Hit
NAGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1020.1281342646098186No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC1020.1281342646098186No Hit
NAGGAAANAGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1010.1268780463293302No Hit
NNGGAAANNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1010.1268780463293302No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1000.12562182804884175No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1000.12562182804884175No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC990.12436560976835334No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT990.12436560976835334No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA990.12436560976835334No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG980.12310939148786493No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG970.12185317320737653No Hit
NAGGAAANAGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA970.12185317320737653No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA960.12059695492688809No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT960.12059695492688809No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT930.11682830008542283No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA920.11557208180493442No Hit
NAGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT910.11431586352444602No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG910.11431586352444602No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG900.11305964524395758No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC900.11305964524395758No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA890.11180342696346918No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT890.11180342696346918No Hit
NNGGAAANNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA880.11054720868298076No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT880.11054720868298076No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA870.10929099040249235No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG850.10677855384151551No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC840.1055223355610271No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT840.1055223355610271No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC840.1055223355610271No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA830.10426611728053867No Hit
NAGGAANNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA830.10426611728053867No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA830.10426611728053867No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC830.10426611728053867No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA820.10300989900005025No Hit
NNGGANNNNGNTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG820.10300989900005025No Hit
NNGGANNNNGNTATGNNNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC820.10300989900005025No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA810.10175368071956184No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT810.10175368071956184No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT810.10175368071956184No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT810.10175368071956184No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG810.10175368071956184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)