FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88545
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC3570.40318482127731664No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG2970.3354226664407928No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2840.3207408662262127No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2640.29815348128070474No Hit
NNGGANNNNGNTATGANNACCTGGCAGCCCCAAATCTGCGGGTGACCACG2630.2970241120334294No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGACACCCAAGACA2610.2947653735388785No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2610.2947653735388785No Hit
NNGGANNNNGNTATGNNNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA2440.2755660963351968No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2190.24733186515331188No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2100.23716754192783332No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2090.23603817268055793No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2000.22587384945507932No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2000.22587384945507932No Hit
NNGGANNNNGNTATGANNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA1990.22474448020780394No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1980.22361511096052855No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1970.22248574171325314No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1850.2089333107459484No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1840.20780394149867298No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1770.1998983567677452No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1760.19876898752046981No Hit
NNGGANNNNGNTATGANNCTCACCAAGAGTGCCCCACGACACCCAAGACA1660.18747529504771585No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1630.18408718730588966No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1600.18069907956406345No Hit
NNGGANNNNGNTATGANNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1580.17844034106951268No Hit
NAGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1550.1750522333276865No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1540.1739228640804111No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA1520.1716641255858603No Hit
NNGGANNNNGNTATGNNNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1460.1648879101022079No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1460.1648879101022079No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1450.16375854085493252No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1450.16375854085493252No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1440.16262917160765714No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1440.16262917160765714No Hit
NAGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGACACCCAAGACA1420.16037043311310634No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1420.16037043311310634No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCCAGGAAACAGCT1400.15811169461855554No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1400.15811169461855554No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1390.15698232537128012No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1380.15585295612400474No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT1370.15472358687672935No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1370.15472358687672935No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1360.15359421762945394No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1340.15133547913490317No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1340.15133547913490317No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1270.14342989440397538No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1260.1423005251567No Hit
NNGGANNNNGNTATGNNNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1260.1423005251567No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1260.1423005251567No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1190.1343949404257722No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1190.1343949404257722No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT1190.1343949404257722No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1160.13100683268394603No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1130.12761872494211984No Hit
NAGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1120.12648935569484443No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCCAGGAAACAGCT1120.12648935569484443No Hit
NAGGANNNNGNTATGANNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1100.12423061720029363No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1100.12423061720029363No Hit
NAGGANNNNGNTATGNNNAGTGTGAAGTCTGGATCAAATATTGCCAGGAA1100.12423061720029363No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1100.12423061720029363No Hit
NNGGANNNNGNTATGANNGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT1070.12084250945846745No Hit
NNGGANNNNGNTATGNNNCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT1060.11971314021119205No Hit
NNGGANNNNGNTATGNNNAAGTTGGTCAGCCCTGGTGACCAGTGAGCGTA1040.11745440171664126No Hit
NNGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCACCTTGCTTC1040.11745440171664126No Hit
NAGGANNNNGNTATGANNCTCACCAAGAGTGCCCCACGACACCCAAGACA1040.11745440171664126No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1040.11745440171664126No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1030.11632503246936587No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGACACCTCCTCTGG1020.11519566322209045No Hit
NNGGANNNNGNTATGNNNGATGGCAGAATGGAAACATGGCAGCTGCCTTG1010.11406629397481506No Hit
NNGGANNNNGNTATGANNCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT990.11180755548026428No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT970.10954881698571348No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT970.10954881698571348No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT970.10954881698571348No Hit
NAGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC950.10729007849116269No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT940.10616070924388729No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCCTCCCCACCTCC940.10616070924388729No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG940.10616070924388729No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT940.10616070924388729No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA930.1050313399966119No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT930.1050313399966119No Hit
NNGGANNNNGNTATGNNNACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG930.1050313399966119No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC920.10390197074933649No Hit
NNGGANNNNGNTATGNNNGTGCAGTCCTCAGGCCACACCTCCAACCCCGT920.10390197074933649No Hit
NAGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT910.10277260150206109No Hit
NNGGANNNNGNTATGANNCCTGGACGCAATCACCCAGGCTGCAGTGCGGT900.10164323225478569No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA900.10164323225478569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)