FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141294
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA36462.580435121095022No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC36292.5684034707772447No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG33682.383682251192549No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG30502.1586196158364825No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA28952.048919274703809No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG27821.9689441872974083No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA27191.924356306707999No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT21841.5457131937661897No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA21291.5067872662674988No Hit
TGTAAAACGACGGCCAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG20781.4706923153141678No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG20711.4657381063597887No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA20401.4437980381332542No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG19721.3956714368621457No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC18971.3425906266366583No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC18841.3333899528642406No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC18771.3284357439098617No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT18731.3256047673645024No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC17401.231474797231305No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC16501.1677778249607202No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC16311.1543306863702634No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC15591.1033731085537957No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT15581.1026653644174558No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT15211.0764788313728821No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT14381.017736068056676No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG14191.0042889294662194No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG13500.955454584058771No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC13340.9441306778773337No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC13230.9363454923775957No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG13030.9221906096507991No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT12470.8825569380157685No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG11510.8146135009271449No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG11000.7785185499738134No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC10450.7395926224751228No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9930.7027899273854517No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA9640.6822653474315965No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC9540.6751879060681982No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT9370.6631562557504211No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG9360.6624485116140812No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG9310.6589097909323821No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT9250.6546633261143431No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG8810.6235225841153905No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT8640.6114909337976135No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA8610.609367701388594No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC8360.5916740979800983No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG8080.5718572621625829No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG8010.5669030532082042No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT7950.5626565883901652No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG7800.5520404263450678No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG7550.534346822936572No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC7460.5279771257095135No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC7290.5159454753917363No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG7230.5116990105736974No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA7030.4975441278469008No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT6950.4918821747561822No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT6600.4671111299842881No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT5990.42393873766755846No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA5990.42393873766755846No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG5820.4119070873497813No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG5720.404829645986383No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5470.38713604257788725No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG5450.3857205543052076No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5320.3765198805327898No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC5300.3751043922601101No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5170.3659037184876923No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT5160.3651959743513525No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4870.34467139439749744No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4740.3354707206250796No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG4670.3305165116707008No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4520.3199003496256033No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC4330.30645321103514656No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4230.2993757696717483No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA3880.2746047248998542No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA3800.26894277180913556No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3720.2632808187184169No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG3590.2540801449459991No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3480.246294959446261No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCTTCCACTGTCC3450.2441717270372415No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG3390.23992526221920252No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3320.23497105326482368No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT3230.2286013560377652No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG3160.2236471470833864No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3000.2123232409019491No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG2980.21090775262926947No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC2940.20807677608391018No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG2860.2024148229931915No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2790.19746061403881268No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2720.19250640508443387No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACACGTGCCAT2650.18755219613005505No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGTGTAAAACGACG2650.18755219613005505No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC2600.18401347544835592No Hit
TGTAAAACGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG2580.18259798717567624No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2510.17764377822129743No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGACCTTGGTAGGAT2400.1698585927215593No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGAGTGCAGTAGT2350.16631987203986015No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT2290.16207340722182118No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGCAGGAAGCACCT2260.15995017481280166No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2160.1528727334494034No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACGTTGCTACCGG2150.15216498931306355No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA2100.14862626863136438No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2100.14862626863136438No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGCGTCATGGCT2090.14791852449502457No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG1990.14084108313162624No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA1920.13588687417724746No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTCCTGCTCA1860.13164040935920845No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1840.1302249210865288No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1800.12739394454116948No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT1620.11465455008705253No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1620.11465455008705253No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA1590.11253131767803304No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCACGTGTCGCCC1530.10828485285999405No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTGGGGAGGCAG1520.10757710872365422No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGACCCACGAGGGCT1510.10686936458731439No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1460.10333064390561525No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT1440.10191515563293559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)