FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141294
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT35032.479227709598426No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT31542.2322250060158253No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT30552.162158336518182No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT28041.9845145582968844No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG26401.8684445199371524No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT26391.8677367758008125No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC26301.861367078573754No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA25661.816071453848005No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT25231.7856384559853924No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC20271.4345973643608363No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA20211.4303508995427971No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC19551.3836397865443684No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT19071.3496680680000566No Hit
CAGGAAACAGCTATGACCAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG18941.3404673942276388No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG17561.2427987034127423No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG17511.239259982731043No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC17451.235013517913004No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC17391.230767053094965No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT16851.1925488697326143No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT14881.0531232748736676No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG14831.0495845541919684No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA14591.0325986949198125No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA14060.9950882556938015No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT14050.9943805115574618No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT13380.9469616544226931No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA12290.8698175435616515No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC12080.8549549166985151No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT11160.7898424561552507No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA11080.7841805030645321No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC9970.705620903930811No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC9700.6865118122496355No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA9690.6858040681132956No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC9550.6758956502045381No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT9070.6419239316602262No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT9030.6390929551148669No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG8710.6164451427519922No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC8270.5853044007530397No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG8050.5697340297535635No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8050.5697340297535635No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT7950.5626565883901652No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC7890.5584101235721262No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT7590.5371777994819313No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG7490.5301003581185331No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG7140.5053293133466389No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG7060.4996673602559203No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC6970.49329766302886174No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6800.4812660127110847No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT6460.4572027120755304No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC6270.44375557348507366No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT5980.4232309935312186No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCACTGGCCGTCGTT5640.3991676928956644No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5090.3602417653969737No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC5080.3595340212606339No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4880.3453791385338373No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4830.3418404178521381No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC4560.3227313261709627No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC4560.3227313261709627No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA4390.3106996758531856No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4240.30008351380808806No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT4170.2951293048537093No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC4050.2866363752176313No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA3970.28097442212691265No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG3950.279558933854233No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT3940.2788511897178932No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT3800.26894277180913556No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3480.246294959446261No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT3470.24558721530992117No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT3470.24558721530992117No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC3460.24487947117358133No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGGAAGGTCCCCA3390.23992526221920252No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3280.23214007671946438No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3260.23072458844678473No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3230.2286013560377652No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGAAACAGCTATG3220.22789361190142537No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT3040.21515421744730845No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA2970.21020000849292964No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2940.20807677608391018No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT2930.20736903194757034No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2910.20595354367489066No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACGCACGGC2820.1995838464478322No Hit
CAGGAAACAGCTATGACCAGTGTGAAGTCTGGATCAAATATTGCCAGGAA2810.19887610231149236No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCACGTGTATCTC2720.19250640508443387No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC2650.18755219613005505No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG2620.18542896372103557No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT2530.1790592664939771No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA2500.1769360340849576No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA2460.1741050575395983No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2450.17339731340325845No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA2420.17127408099423896No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC2410.17056633685789913No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2360.1670276161762No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCGTCTCTA2310.16348889549450082No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTTCCTGCAACAC2280.16136566308548134No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTAGCTGCCTATC2240.158534686540122No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA2200.15570370999476268No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2170.15358047758574322No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2140.1514572451767237No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2070.1465030362223449No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2060.14579529208600508No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA1980.14013333899528643No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA1910.13517913004090762No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG1840.1302249210865288No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1820.12880943281384916No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC1790.12668620040482964No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCATCGCTACACTC1780.1259784562684898No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1760.12456296799581015No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1740.1231474797231305No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1670.11819327076875168No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA1650.11677778249607203No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGACTCTTCCCAGTG1610.11394680595071269No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA1580.1118235735416932No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC1540.10899259699633389No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCCTCGTGGGTC1510.10686936458731439No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT1490.10545387631463474No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG1450.10262289976927541No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1430.10120741149659575No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC1430.10120741149659575No Hit
CAGGAAACAGCTATGACCGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG1420.10049966736025591No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)