FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156049
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA58003.7167812674224123No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA56053.5918205179142446No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG52663.3745810610769693No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA47113.0189235432460317No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG46212.961249351165339No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG39802.550480938679517No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA37212.384507430358413No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT35862.2979961422373743No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT32652.0922915238162374No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT32592.0884465776775243No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA30041.9250363667822286No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC29891.9154240014354464No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT29891.9154240014354464No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA28591.8321168350966686No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT25691.646277771725548No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG25011.6027017154868022No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG24691.5821953360803338No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG22071.414299354689873No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT20731.3284288909252862No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG19881.2739588206268544No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC17291.1079853123057501No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG17111.0964504738896117No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA15871.0169882536895463No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG15600.9996859960653385No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT14610.9362443847765766No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC14530.9311177899249595No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG14290.9157380053701082No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT14170.9080481130926824No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT13880.8894642067555704No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG12940.8292267172490693No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC12320.7894956071490365No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC12210.782446539228063No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT11840.7587360380393338No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC11800.7561727406135252No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA11780.754891091900621No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT10160.6510775461553743No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA9790.6273670449666451No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG9410.6030157194214638No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG9030.5786643938762824No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG8050.5158636069439727No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC7970.5107370120923556No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT7860.5036879441713821No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC7610.4876673352600786No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT7520.48189991605200927No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC7460.47805496991329643No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCACCACCCCTGGC7440.4767733212003922No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA7410.47485084813103573No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG7130.4569077661503758No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG6810.4364013867439074No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG6610.42358489961486456No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA6410.4107684124858218No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA6050.38769873565354473No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG5930.380008843376119No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5820.3729597754551455No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5720.3665515318906241No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG5670.3633474101083634No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG4920.3152855833744529No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC4860.31144063723574006No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT4820.3088773398099315No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAACCCCTGCCA4710.30182827188895794No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT4680.29990579881960155No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4630.29670167703734085No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC4600.29477920396798446No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA4330.2774769463437766No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4300.27555447327442023No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGGGCTGCTCTGTC4280.27427282456151597No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT4210.26978705406635095No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG4150.2659421079276381No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG4000.256329742580856No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG3970.2544072695114996No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC3960.2537664451550474No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3950.2531256207985953No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3740.2396683093131004No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3620.2319784170356747No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3590.2300559439663183No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGATTCCAGCTAAGCG3580.22941511960986616No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3570.22877429525341397No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGGATGGAGGGG3430.21980275426308404No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGCACTGGG3230.20698626713404125No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCGCCTCCTGCTCAGCC3200.20506379406468483No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG3180.20378214535178052No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGCGCCGGGGGCAG3150.2018596722824241No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACATCTCCTGA3050.19545142871790272No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA3010.19288813129209414No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG3000.19224730693564202No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG2880.18455741465821632No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA2830.18135329287595564No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2770.17750834673724278No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT2670.1711001031727214No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2540.16276938653884357No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC2480.15892444040013073No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG2420.15507949426141787No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG2370.1518753724791572No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGGGCAGGCAGAATGT2340.14995289940980078No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG2330.14931207505334862No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG2260.14482630455818366No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGTTTCCTGGGGG2210.14162218277592295No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2090.13393229049849725No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG1900.12175662772590659No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC1850.1185525059436459No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGTAGTGC1850.1185525059436459No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGTGGGGTTAGG1790.11470755980493308No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG1650.10573601881460311No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1600.10253189703234242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)