FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003246686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157520
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT49293.1291264601320465No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT34252.174327069578466No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC32492.0625952260030473No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT27151.723590655154901No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT26771.6994667343829355No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC25881.6429659725749113No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA25521.6201117318435754No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC24921.5820213306246826No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC23181.4715591670898933No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT21871.388395124428644No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC21541.367445403758253No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT20871.324911122397156No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG19091.2119095987811073No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG17881.1350939563230067No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG17831.1319197562214323No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG17091.0849415947181311No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC16861.070340274250889No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA16711.0608176739461657No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA16281.0335195530726256No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC14120.8963941086846114No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG13320.8456069070594211No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC12970.8233875063484003No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG12020.7630777044184865No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG11990.7611731843575419No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG11270.7154647028948704No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC9820.6234128999492128No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT9730.6176993397663788No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC9630.6113509395632301No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT9580.6081767394616556No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG9450.5999238191975622No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT9440.5992889791772473No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA9330.5923057389537837No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT9120.5789740985271711No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT8980.5700863382427628No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA8930.5669121381411883No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC8920.5662772981208736No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG8390.5326307770441849No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG8380.53199593702387No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT8110.5148552564753682No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC8030.5097765363128491No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG8030.5097765363128491No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG7850.4983494159471813No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT7810.4958100558659218No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT7780.49390553580497715No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA7440.47232097511427124No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT7370.4678770949720671No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGACACCCAAGACA7230.4589893346876587No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC7180.45581513458608436No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT6660.42280345352971055No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6300.3999492127983748No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG6250.3967750126968004No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT6240.39614017267648555No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC6200.39360081259522606No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC5860.37201625190452003No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC5800.36820721178263077No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5800.36820721178263077No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG5680.3605891315388522No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG5650.3586846114779076No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG5640.3580497714575927No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5640.3580497714575927No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC5630.3574149314372778No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG5520.3504316912138141No Hit
CAGGAAACAGCTATGACCCTGTCCCAAGGGAGCAAGCTCAATAAAGCATA5510.34979685119349924No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACACCTCCTCTGG5450.34598781107161No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCGGGTGACCACG5320.3377348908075165No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT5150.32694261046216355No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC5110.324403250380904No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA5100.3237684103605891No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGTCATTGGTG4820.30599288979177247No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTACTCAAGG4810.3053580497714576No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4750.3015490096495683No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG4700.2983748095479939No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4660.2958354494667344No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG4560.28948704926358554No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA4530.28758252920264094No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT4510.28631284916201116No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT4500.2856780091416963No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT4450.2825038090401219No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCGCTCTCCTCCC4420.28059928897917724No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT4420.28059928897917724No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC4370.2774250888776028No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCAGGACACCCCA4210.26726764855256474No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTTTTACACCA4210.26726764855256474No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTTCGTGGGGAA4120.2615540883697308No Hit
CAGGAAACAGCTATGACCCCCCTCCGCTGGTTGAATGGGCTGTCTTGGCT4060.25774504824784156No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA4030.2558405281868969No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA4020.255205688166582No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGTGATGG4010.2545708481462671No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA3990.2533011681056374No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3980.2526663280853225No Hit
CAGGAAACAGCTATGACCCTGTCCCAAGGGAGCAAGCCAGGAAACAGCTA3850.24441340782122906No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGCCAACGGTGC3840.24377856780091414No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCGCCTGTCACC3780.2399695276790249No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC3750.23806500761808022No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT3630.2304469273743017No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3550.22536820721178263No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGACCGTCGTT3540.22473336719146775No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGCCTCCCGACT3530.22409852717115286No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA3530.22409852717115286No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3480.22092432706957849No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCCCCGA3450.21901980700863383No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGCTGCTGCGAGT3420.2171152869476892No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3390.21521076688674454No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGCGGCGCTCCCTG3370.21394108684611476No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC3360.2133062468257999No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC3260.20695784662265107No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA3250.2063230066023362No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC3200.20314880650076178No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC3160.20060944641950232No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCAGTGCGGTTCC3160.20060944641950232No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGACGTGTCCGCT3130.19870492635855763No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT3130.19870492635855763No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3100.19680040629761303No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGTCCTGGCGT3050.1936262061960386No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC3020.19172168613509397No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2990.1898171660741493No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA2980.18918232605383442No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2910.18473844591163027No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACACACACTTCTAA2800.17775520568816658No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT2720.17267648552564754No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2680.17013712544438803No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2650.16823260538344337No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCTGAGAGCCTGTG2630.1669629253428136No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCTCCCTTTGG2630.1669629253428136No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGGCGCAGCCACC2610.16569324530218385No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGAGAGGC2610.16569324530218385No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTGTGTCATTGGT2580.16378872524123922No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG2530.16061452513966482No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCAGGAAACAGCA2520.15997968511934993No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2480.1574403250380904No Hit
CAGGAAACAGCTATGACCTCTGCAGCTAGCTGGGAACTGGCCGTCGTTTT2460.15617064499746064No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2420.1536312849162011No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC2410.15299644489588624No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2410.15299644489588624No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCCCAAGACACTG2390.1517267648552565No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCCTTTGGGCT2390.1517267648552565No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCGCAGTGGGGCCA2380.1510919248349416No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTGCCTCATGTCC2340.14855256475368206No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA2330.1479177247333672No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2310.14664804469273743No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTCCCTTTGGGC2290.14537836465210766No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC2260.14347384459116302No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA2260.14347384459116302No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2240.14220416455053328No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG2200.13966480446927373No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2180.138395124428644No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGGTACCCGTGTC2180.138395124428644No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT2150.13649060436769933No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCTCCCTTTGGG2130.13522092432706959No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGCTCCCCAGG2130.13522092432706959No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC2100.13331640426612493No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATCCACCTGCTCTG2100.13331640426612493No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGGTTCC1990.12633316404266126No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG1980.12569832402234637No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCAGAGGGCGGCCA1910.12125444388014221No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1860.11808024377856781No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGAGATGAATTTGA1860.11808024377856781No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT1830.11617572371762316No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG1820.11554088369730828No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA1740.11046216353478924No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGGGGCCCTCTTCC1730.10982732351447436No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCAGGAAACAGCTA1660.10538344337227018No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC1640.10411376333164042No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCCTCAAGTCCCC1640.10411376333164042No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT1630.10347892331132554No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1600.10157440325038089No Hit
CAGGAAACAGCTATGACCAACTTTTAGGTAGAGCCAAAGAACTGGCCGTC1590.10093956323006602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)