FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003246688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003246688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71588
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT4330.6048499748561211No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC3490.48751187349835173No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2680.3743644186176454No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2670.37296753645862435No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT2640.36877688998156116No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC2560.35760183270939266No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2480.3464267754372241No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2470.34502989327820305No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC2330.3254735430519081No Hit
NAGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT2300.32128289657484493No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2120.29613901771246576No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1930.2695982566910656No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1820.25423255294183383No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1780.24864502430574958No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1740.24305749566966528No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1700.23746996703358103No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1680.2346762027155389No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1680.2346762027155389No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1670.23327932055651787No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1630.22769179192043357No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1630.22769179192043357No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1610.22489802760239144No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1610.22489802760239144No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1580.22070738112532828No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1560.21791361680728616No Hit
NAGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1510.21092920601218082No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1480.2067385595351176No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1470.20534167737609657No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1460.20394479521707548No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1430.19975414874001232No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1420.19835726658099123No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1400.1955635022629491No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1330.18578532714980164No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1330.18578532714980164No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1320.18438844499078058No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1310.18299156283175952No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1310.18299156283175952No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1270.17740403419567527No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1260.1760071520366542No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1260.1760071520366542No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1240.17321338771861206No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1210.16902274124154887No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1180.16483209476448568No Hit
NAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1170.1634352126054646No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1150.16064144828742247No Hit
NAGGAANNNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT1140.1592445661284014No Hit
NAGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1130.15784768396938034No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1120.15645080181035928No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1110.1550539196513382No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1090.15226015533329607No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1060.14806950885623288No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1060.14806950885623288No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1030.1438788623791697No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1030.1438788623791697No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1020.14248198022014863No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1020.14248198022014863No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1020.14248198022014863No Hit
NAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1020.14248198022014863No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1010.14108509806112757No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1000.13968821590210648No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC990.13829133374308542No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT990.13829133374308542No Hit
NNGGAAANNGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT980.13689445158406435No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC980.13689445158406435No Hit
NNGGANANAGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT970.1354975694250433No Hit
NAGGAAANAGNTATGANNCTCGTGCAGTCTGGTGTCCGCAGGAAACAGCT970.1354975694250433No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC970.1354975694250433No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC950.13270380510700117No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC940.1313069229479801No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT940.1313069229479801No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT940.1313069229479801No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA930.12991004078895904No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT920.12851315862993798No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC910.12711627647091692No Hit
NAGGAANNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC900.12571939431189585No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC900.12571939431189585No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC890.12432251215287479No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC890.12432251215287479No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA890.12432251215287479No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA880.1229256299938537No Hit
NAGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC870.12152874783483264No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACGGAGATCCAGCC870.12152874783483264No Hit
NAGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC870.12152874783483264No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT870.12152874783483264No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT870.12152874783483264No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG860.12013186567581158No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA860.12013186567581158No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT840.11733810135776945No Hit
NNGGAAANNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT840.11733810135776945No Hit
NAGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT840.11733810135776945No Hit
NAGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG830.11594121919874839No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA820.11454433703972733No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA820.11454433703972733No Hit
NAGGAANNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT820.11454433703972733No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC820.11454433703972733No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT810.11314745488070627No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT810.11314745488070627No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATTTTCATGT810.11314745488070627No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT810.11314745488070627No Hit
NAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA800.1117505727216852No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT790.11035369056266414No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC780.10895680840364308No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT780.10895680840364308No Hit
NNGGAAANNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA780.10895680840364308No Hit
NAGGAANNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA770.10755992624462202No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG770.10755992624462202No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT760.10616304408560093No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC750.10476616192657986No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT750.10476616192657986No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT750.10476616192657986No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA750.10476616192657986No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC740.1033692797675588No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC730.10197239760853774No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA720.10057551544951668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)